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Human IgG4-S228P Isotype Control Antibody

hIgG4 Isotype Control, Human IgG4 Isotype Control, hIgG4 S228P, hIgG4 SPLEPG Mutant

Catalog No. Product Name Size List Price (US$) Quantity
PA007128 In Vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody 1 mg 195.00
PA007128 In Vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody 5 mg 695.00
PA007128 In Vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody 25 mg 2295.00
PA007131 In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody 1 mg 295.00
PA007131 In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody 5 mg 995.00
PA007131 In Vivo Grade Recombinant Human IgG4-S228P L235E P329G (SPLEPG) Isotype Control Antibody 25 mg 2950.00
Description

The ideal recombinant human IgG4 isotype control antibody (hIgG4 S228P kappa isotype control) has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG4 S228P kappa constant regions. The human IgG4 kappa isotype control and mutants can be used in the cell-based assay or animal model as the antibody negative control when evaluating fully human, humanized, or chimeric antibodies.

The hIgG4 isotype control mutants were generated because the corresponding mutations had been introduced to some human IgG4 therapeutic antibody candidates. For example, S228P, an engineered mutation in the core hinge region, is used to prevent Fab-arm exchange in vivo of human IgG4 molecules; P329G substitution combined with S228P and L235E was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface. When clients test their engineered antibody candidates, the silent Fc effctor function human IgG4 isotype controls are useful for in vivo use.

Welcome to contact us and ask for a quote for the engineered human IgG4 isotype control mutants, hIgG4 isotype control and Fc silenced mutants with Avi-, His-, and Flag-tags, and biotinylated hIgG4 isotype control and silent Fc mutants. A variety of conjugates (such as dyes and fluorophores) with the human IgG4 isotype control and silent Fc effctor function mutants are available.

PA007128: In Vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody (hIgG4 Isotype Control)

The recombinant human IgG4-S228P kappa isotype control antibody (hIgG4 S228P isotype control) was produced in CHO cells.
Immunogen: Trinitrophenol (TNP).
Clone: 1R12.
Isotype: human IgG4, kappa.
Applications: an isotype-matched negative control for human IgG4 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution (1 mg/ml) of PBS, pH 7.0.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007131: In Vivo Grade Recombinant Human IgG4-S228P L235E P329G Isotype Control Antibody (hIgG4 SPLEPG Isotype Control Mutant)

The recombinant human IgG4-S228P L235E P329G isotype control antibody (hIgG4 SPLEPG isotype control mutant, Fc silenced) was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17.
Isotype: human IgG4, kappa.
Applications: an isotype-matched negative control for human IgG4 SPLEPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of PBS, pH 7.0.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant hIgG4 kappa isotype control and mutants are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Isotype control antibodies are used as negative controls of primary antibodies to check presence of non-specific binding to FC receptors on the cell surface, other cellular proteins, carbohydrates, and lipids. Thus, the isotype control antibody normally matches the host species and isotype, including light chains (such as human IgG4 S228P kappa antibody), concentration, and conjugation format of the experimental primary antibody. The ideal human isotype control antibody has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG constant regions.

Isotype control antibodies are frequently used in IHC and Flow cytometry experiments, and in vivo animal study.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Kappa Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Kappa Isotype Control Antibody
In vivo Grade Recombinant Mouse IgG1 Kappa Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Kappa Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human Fc Protein (IgG1)
In vivo Grade Recombinant Human Fc Protein (IgG2)
In vivo Grade Recombinant Human Fc Protein (IgG4)
In vivo Grade Recombinant Mouse Fc Protein (IgG1)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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