My Cart [ 0 ]
Home > Antibodies > Anti-Human Antibodies (In Vivo) > Anti-Human CD3 Monoclonal Antibody (Clone: UCHT1)

Anti-Human CD3 Monoclonal Antibody (Clone: UCHT1)

Cluster of Differentiation 3, CD3ε (Epsilon), CD3D, CD3E, CD3G, T-Cell Surface Glycoprotein CD3

Catalog No. Product Name Size List Price (US$) Quantity
PA007197 In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Mouse IgG1 Kappa (Clone: UCHT1) 1 mg 150.00
PA007197 In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Mouse IgG1 Kappa (Clone: UCHT1) 5 mg 350.00
PA007197 In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Mouse IgG1 Kappa (Clone: UCHT1) 25 mg 900.00
PA007197.h1 In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Human IgG1 Kappa (Clone: UCHT1) 1 mg 150.00
PA007197.h1 In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Human IgG1 Kappa (Clone: UCHT1) 5 mg 350.00
PA007197.h1 In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Human IgG1 Kappa (Clone: UCHT1) 25 mg 900.00
Description

Background of Recombinant Anti-Human CD3 Antibody (Clone: UCHT1)

The mouse anti-human CD3 monoclonal antibody (clone No. UCHT1, mouse IgG1 kappa) reacts with the human CD3ε (epsilon) chain protein (T3 or cluster of differentiation 3) encoded by the human CD3E gene, a critical signal-transduction component of the T-cell receptor (TCR) complex. CD3 is expressed uniformly on all mature human T lymphocytes and plays an indispensable role in adaptive immune responses. It has been shown that in advanced immunooncology profiling and structural biology models, specific cellular signaling pathways can be evaluated and blocked via anti-human CD3 antibody treatment and receptor-ligand competitive profiling. The UCHT1 functional antibody effectively binds to the conformational epitope of the human CD3 complex, serving as an industry-standard molecular tool during specialized preclinical investigations.

Our recombinant anti-human cd3 ucht1 antibody is engineered with 100% precise amino acid sequences of the variable regions derived from the original hybridoma source. Expressed in advanced mammalian cells, this in vivo grade ucht1 antibody delivers superior lot-to-lot consistency with verified high binding affinity and excellent structural stability. This anti-human cd3 clone ucht1 mouse igg1 reagent is supplied in a specialized carrier-free matrix as a low endotoxin anti-human cd3 ucht1 formulation (<1 EU/mg, azide-free, no stabilizers) to eliminate non-specific cell activation or background cytotoxicity. This makes it the definitive premium standard for high-throughput anti-cd3 ucht1 neutralization assays, biological pathway tracking, pharmacokinetic (PK) evaluations, and advanced bioanalytical anti-drug antibody (ADA) screening cascades.

PA007197: In vivo Grade Recombinant Anti-human CD3 Mouse IgG1 Kappa Monoclonal Antibody (Clone: UCHT1)

Recombinant mouse IgG1 Monoclonal Antibody.
Clone: UCHT1.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: UCHT1) specifically binds to the human T cell receptor CD3.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD3 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Syd Labs Recombinant Anti-Human CD3 Monoclonal Antibody (Clone: UCHT1)

Wei J, et al. Mol Ther Oncolytics 2022. doi: 10.1016/j.omto.2022.09.006
“...This study evaluates a DNA-launched bispecific T cell engager (BiTE) targeting IL-13Rα2 in a humanized NSG mouse model engrafted with human PBMCs. Flow cytometry analysis utilized anti-human CD45 (HI30), anti-human CD3 (UCHT1), anti-human CD4 (OKT4) …… The IL-13Rα2 BiTE significantly reduced tumor growth compared to controls.”
(Tags: anti-human CD3e UCHT1; anti-human CD3e UCHT1 in vivo)
Argüello RJ, et al. Cell Metab 2020. doi: 10.1016/j.cmet.2020.11.007
“This study introduces SCENITH, a flow cytometry-based method to profile energy metabolism at single-cell resolution. Immune cell phenotyping was performed using antibodies including anti-CD45 (HI30), anti-CD3 (UCHT1), anti-CD4 (RPA-T4) …… SCENITH measures protein synthesis rates as a proxy for cellular energy metabolism, validated in human immune cells.”
(Tags: anti-human CD3e UCHT1 antibody; bioactivity of anti-human CD3e UCHT1)
Kellner S, et al. EMBO Mol Med 2020. doi: 10.15252/emmm.201911902
“This study investigates an IgG-based bispecific antibody in NSG mice engrafted with human PBMCs and tumor cells. T cells were stained with anti-CD3 (UCHT1) and anti-CD8 (SK1) antibodies for flow cytometry analysis …… peripheral blood analyzed for T cell activation and cytokine release post-treatment.”
(Tags: anti-human CD3e UCHT1 of low endotoxin; anti-human CD3e UCHT1 antibody of low endotoxin)
Wang C, et al. Cancer Immunol Res 2014. doi: 10.1158/2326-6066.CIR-14-0040
“This study characterizes the anti-PD-1 antibody nivolumab in vitro and evaluates its toxicology in cynomolgus monkeys. Flow cytometry was performed using anti-CD3 (UCHT1), anti-CD4 (L200), and anti-CD8 (RPA-T8) antibodies …… Nivolumab binding to PD-1 was confirmed on activated T cells.”
(Tags: anti-human CD3e antibody (UCHT1); anti-human CD3e UCHT1)
Staroverov RMT, et al. Mol Cancer Ther 2009. doi: 10.1158/1535-7163.MCT-08-1160
“This study evaluates light-activatable UCHT1-folate conjugates in nude mice bearing ovarian cancer xenografts. Flow cytometry confirmed T cell binding using anti-CD3 (UCHT1) antibody …… The conjugate significantly reduced tumor growth compared to non-irradiated controls.”
(Tags: clone UCHT1 of anti-human CD3e mAb; UCHT1 anti-human CD3e antibody)
For more technical references or customized data sheets regarding the In Vivo Grade Recombinant Anti-Human CD3 Monoclonal Antibody (Clone: UCHT1) formats, please contact our scientific support team at message@sydlabs.com.

Frequently Asked Questions (FAQ) about In Vivo Grade Anti-Human CD3 Antibody (Clone: UCHT1)

Q: What are the main applications of Syd Labs' functional grade UCHT1 antibody?

Our functional grade ucht1 antibody is explicitly tailored for sensitive preclinical and analytical workflows. It is thoroughly validated for anti-cd3 ucht1 neutralization assays, cellular pathway blockades, and high-throughput bioanalytical configurations. Because of its defined recombinant nature and superior target specificity, it serves as an excellent benchmark for therapeutic antibody development, including pharmacokinetic (PK) modeling and anti-drug antibody (ADA) assay optimization.

Q: Is this recombinant anti-human CD3 UCHT1 antibody supplied with preservatives?

No, this formulation is entirely carrier-free and preservative-free. To protect fragile primary cells and animal models from chemical toxicity, our low endotoxin anti-human cd3 ucht1 matrix contains absolutely no sodium azide and no BSA or stabilizing protein additives. It is supplied as a 0.2 μM sterile-filtered liquid in pure 1x PBS, maintaining an endotoxin threshold of less than 1 EU/mg via LAL testing.

Q: What is the specific host species and isotype for clone UCHT1?

This product is engineered as an anti-human cd3 clone ucht1 mouse igg1 (kappa) monoclonal antibody. Every batch features a verified structural purity >95% by SDS-PAGE. Knowing the exact Mouse IgG1 isotype allows investigators to perfectly align their isotype control strategies and calculate non-specific Fc-receptor binding factors during comparative immune pathway tracking.

Q: Can I use this in vivo grade UCHT1 page for routine multi-color flow cytometry?

While this antibody possesses exceptional binding affinity to the human CD3 epsilon subunit, this specific page (p15855.htm) delivers a high-value, preservative-free format intended for functional biology and ucht1 clone pharmacokinetic ada assays. If you only require routine leukocyte screening or multi-color immunophenotyping, we strongly suggest using our detection-tier UCHT1 format on page p15806.htm, which is pre-stabilized with preservatives for continuous laboratory pipetting.

Q: What are the optimal storage guidelines to preserve the neutralization activity of this antibody?

To avoid structural degradation and protect its binding capabilities, store short-term (up to 4 weeks) at 2–8°C. For long-term programmatic inventory up to 12 months, we highly recommend dividing the stock into single-use working aliquots and storing at -20°C to -70°C in a manual defrost freezer. Strictly avoid repeating freeze-thaw cycles, which induce protein aggregation and ruin functional neutralization efficacy.

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry

Related Links

See our Privacy Policy