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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human HLA-DR/DP/DQ Monoclonal Antibodies for Flow Cytometry

Anti-Human HLA-DR/DP/DQ Monoclonal Antibodies for Flow Cytometry

beta1 domain MHC class II HLA DPB, CELIAC1, class II HLA beta chain, MHC class II HLA-DR, DP, DQ

Catalog No. Product Name Size List Price (US$) Quantity
PA007471.m1 Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Mouse IgG1 Kappa 50 ug 100.00
PA007471.m1 Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Mouse IgG1 Kappa 200 ug 200.00
PA007471.h1Fs Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa 50 ug 200.00
PA007471.h1Fs Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa 200 ug 400.00
PA007767.h1Fs-b Biotinylated Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa 50 ug 150.00
PA007767.h1Fs-b Biotinylated Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa 200 ug 300.00
Description

PA007471.m1: Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Mouse IgG1 Kappa

Recombinant  Mouse IgG1 Monoclonal Antibody. 
Clone: F3.3.
Isotype:  Mouse IgG1 Kappa.
Source: The anti-human HLA-DR/DP/DQ monoclonal antibody (clone: F3.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant F3.3 antibody specifically binds to human HLA-DR/DP/DQ.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007471.h1Fs: Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: F3.3.
Isotype: Human IgG1 kappa.
Source: The anti-human HLA-DR/DP/DQ monoclonal antibody (clone: F3.3) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant F3.3 antibody specifically binds to human HLA-DR/DP/DQ.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007767.h1Fs-b: Biotinylated Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa

Recombinant  human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: F3.3.
Isotype:  Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human HLA-DR/DP/DQ monoclonal antibody (clone: F3.3) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant F3.3 antibody specifically binds to human HLA-DR/DP/DQ.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The F3.3 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3)

HLA antibody affinity determination: From HLA-specific monoclonal antibodies to donor HLA specific antibodies in transplantation.
Hug, K., et al. HLA. 2023 Jul;102(1):3-15. doi: 10.1111/tan.14876. PMID: 37191252
The F3.3 antibody (anti-human HLA-DR/DP/DQ) was used to assess HLA-DR expression on donor lymphocytes via flow cytometry, with PE-conjugated F3.3 staining cells for 30 minutes on ice. Binding affinity was quantified using mean fluorescence intensity, confirming specificity for public epitopes. Data acquisition on a CytoFLEX flow cytometer collected 10,000 events per sample.
(Tags: activity of F3.3 antibody; function of F3.3 antibody)
Recombinant human monoclonal HLA antibodies of different IgG subclasses recognising the same epitope: Excellent tools to study differential effects of isotypes in antibody-dependent cellular cytotoxicity.
Hiemstra, I. H., et al. HLA. 2019 Nov;94(5):441-450. doi: 10.1111/tan.13664. PMID: 31403241
Recombinant F3.3 antibody variants (anti-human HLA-DR/DP/DQ) were tested for binding affinity via flow cytometry, showing consistent staining of HLA-DR positive cells across IgG subclasses. Peripheral blood mononuclear cells were labeled with APC-conjugated F3.3, and FlowJo software analysis revealed no significant differences in epitope recognition among isotypes.
(Tags: F3.3 antibody; F3.3 antibody for animal model)
Production and characterization of chimeric anti-HLA monoclonal antibodies targeting public epitopes as tools for standardizations of the anti-HLA antibody detection.
Haskova, Z., et al. Hum Immunol. 2013 May;74(5):622-9. doi: 10.1016/j.humimm.2013.01.004. PMID: 23352853
Chimeric monoclonal antibodies, including F3.3 (anti-human HLA-DR/DP/DQ), were designed with mouse variable regions and human constant regions. The chimeric F3.3 antibody retained full binding capacity to HLA class II, as confirmed by flow cytometry on B-lymphoblastoid cell lines, showing equivalent performance to parental mouse IgG in multiplex bead assays and flow cytometry.
(Tags: F3.3 antibody for cancer research; F3.3 antibody for flow cytometry)
Chimeric IgA antibodies against HLA class II effectively trigger ADCC and ADC.
Brüggemann, M., et al. Blood. 2007 Feb 1;109(3):1141-8. doi: 10.1182/blood-2006-06-029017. PMID: 17202363
Chimeric F3.3 antibodies (anti-human HLA-DR/DP/DQ), including human IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4 isotypes, were generated using variable regions from the F3.3 hybridoma. Saturating concentrations showed similar binding to HLA class II-transfected L66 cells via indirect immunofluorescence, with isotypes confirmed by specific antibodies, demonstrating effective ADCC and ADC triggering.
(Tags: F3.3 mAb for flow cytometry; F3.3 mAb for mouse tumor model)
For more technical references or data sheets regarding the Anti-Human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant anti-human HLA-DR/DP/DQ monoclonal antibodies for flow cytometry:

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry

Related Links
Anti-human HLA-DR/DP/DQ F3.3 Antibody for Flow Cytometry, Mouse IgG1 Kappa
Anti-Human HLA-DR/DP/DQ Monoclonal Antibody, Mouse IgG1 Kappa (Clone: F3.3)
Anti-human MHC Class Il HLA-DP/DQ/DR Monoclonal Antibody (Clone: IVA12)
Anti-Human HLA-DR Monoclonal Antibodies (Clone: L243)
Anti-Human HLA-DR Monoclonal Antibodies for Flow Cytometry (Clone: L243)
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