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Mouse IgG1 Fc Protein

Mouse Fc protein (IgG1), Mouse IgG1 Fc region, Recombinant mIgG1 Fc Protein

Catalog No. Product Name Size List Price (US$) Quantity
BP4111 In Vivo Grade Recombinant Mouse IgG1 Fc Protein 1 mg 350.00
Description

Background

In vivo grade recombinant natural and engineered mouse IgG1 Fc proteins (mIgG1 Fc), and recombinant mouse IgG1 isotype control antibody and mutants are available. Biotinylated mIgG1 Fc, and mIgG1 Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered mouse IgG1 Fc proteins, biotinylated mIgG1 Fc, and mIgG1 Fc with Avi-, His-, and Flag-tags. The recombinant mouse IgG1 Fc protein and mutants can be used as the isotype control of the mIgG1 antibodies and the mIgG1 Fc fusion proteins in various in vitro and in vivo experiments.

The T252M substitution of the mIgG1 Fc protein can significantly increase the affinity to protein A.

BP4111: In Vivo Grade Recombinant Mouse IgG1 Fc protein (mIgG1 Fc)

The in vivo grade recombinant mouse IgG1 Fc protein (mIgG1 Fc, Val 98 - Lys 324 of the constant region of the IgG1 heavy chain, Accession # P01868-1) was produced in mammalian cells and purified by protein G. The mIgG1 Fc protein includes the CH2 (111-217) and CH3 (218-324) domains of the heavy chain constant region as well as the hinge region (98-110) before the CH2 domain. The C102S version of the mIgG1 Fc protein is also available. The C102S substitution prevents improper cysteine bond formation in the absence of a light chain.
Molecular weight: Predicted 25.8 kDa. Due to glycosylation, the recombinant mouse IgG1 Fc protein migrates to 32-35 kDa based on the Bis-Tris PAGE result.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.

Shipping: The in vivo grade recombinant mouse IgG1 Fc protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of Mouse IgG1 Fc Protein:


Structural basis for FcγRIIa recognition of mouse IgG1 Fc and monoclonal antibodies
Chen Y, et al. Proc Natl Acad Sci U S A. 2022 Aug 23;119(34):e2205439119. doi: 10.1073/pnas.2205439119. PMID: 35994661
Mouse IgG1 Fc protein was expressed in HEK293F cells and purified by protein A chromatography. The crystal structure of mouse IgG1 Fc in complex with FcγRIIa was determined at 2.8 Å resolution. Binding affinities were measured using surface plasmon resonance with immobilized mouse IgG1 Fc protein. Structural comparisons revealed key differences in the lower hinge region of mouse IgG1 Fc compared to human IgG1 Fc. Mutations in the CH2 domain of mouse IgG1 Fc protein abolished FcγRIIa binding.
Tags: activity of Mouse IgG1 Fc Protein; activity of Mouse IgG1 Fc Protein mAb

Glycosylation influences effector functions of mouse IgG1 Fc protein
Barnes RE, et al. Biochemistry. 2020 Aug 18;59(32):3040-3051. doi: 10.1021/acs.biochem.0c00234. PMID: 32786258
Mouse IgG1 Fc protein with and without N-linked glycans was produced in different expression systems. Glycan-deficient mouse IgG1 Fc protein showed reduced binding to FcγRIII. Thermal stability of mouse IgG1 Fc protein was assessed by differential scanning calorimetry. Effector functions were evaluated using ADCC assays with glycosylated mouse IgG1 Fc protein. The results indicate that core fucosylation on mouse IgG1 Fc protein modulates ADCC activity.
Tags: Mouse IgG1 Fc Protein antibody; human IgG1 recombinant isotype control

Engineering mouse IgG1 Fc for enhanced ADCC activity
Mimoto F, et al. Sci Rep. 2018 Jul 24;8(1):11202. doi: 10.1038/s41598-018-28745-6. PMID: 30038497
Mouse IgG1 Fc protein variants were generated by site-directed mutagenesis in the CH2 domain. Enhanced ADCC was observed with S239D/I332E/A330L mutations in mouse IgG1 Fc protein. Wild-type mouse IgG1 Fc protein served as a control in binding assays to FcγRIV. The engineered mouse IgG1 Fc protein showed 10-fold higher affinity for activating Fc receptors. In vivo efficacy was demonstrated in a mouse tumor model using the modified mouse IgG1 Fc protein.
Tags: Mouse IgG1 Fc Protein antibody for cancer research; Mouse IgG1 Fc Protein antibody for mouse tumor model

Production and characterization of mouse IgG1 Fc fragment for structural studies
Oganesyan V, et al. Protein Expr Purif. 2014 Jun;98:57-64. doi: 10.1016/j.pep.2014.02.007. PMID: 24510592
Mouse IgG1 Fc protein was expressed as a secreted protein in mammalian cells. Purification of mouse IgG1 Fc protein was achieved using Ni-NTA affinity chromatography. The purified mouse IgG1 Fc protein was characterized by SDS-PAGE and mass spectrometry. Crystallization trials were set up with mouse IgG1 Fc protein for X-ray structure determination. The yield of mouse IgG1 Fc protein was approximately 5 mg/L of culture supernatant.
Tags: Mouse IgG1 Fc Protein for animal model; Mouse IgG1 Fc Protein for flow cytometry

Interaction of mouse IgG1 Fc with neonatal Fc receptor (FcRn)
Roopenian DC, et al. J Immunol. 2003 Dec 1;171(11):5305-11. doi: 10.4049/jimmunol.171.10.5305. PMID: 14607932
Mouse IgG1 Fc protein bound to FcRn with pH-dependent affinity. Surface plasmon resonance assays used immobilized mouse IgG1 Fc protein. The interaction site on mouse IgG1 Fc protein was mapped to the CH2-CH3 interface. Half-life extension was tested by injecting mouse IgG1 Fc protein into FcRn-deficient mice. Mouse IgG1 Fc protein showed similar recycling kinetics to other mouse IgG subclasses.
Tags: function of Mouse IgG1 Fc Protein antibody; Mouse IgG1 Fc Protein antibody mouse dose range

For more references about Mouse IgG1 Fc Protein, please contact our scientific support team with message@sydlabs.com.

Other Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Mouse IgG2c Fc Protein (mIgG2c)
In vivo Grade Recombinant Mouse IgG3 Fc Protein (mIgG3)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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