Name: Human IgG2 Isotype Control Antibody
Brand: Syd Labs
SKU: PA007127
Price: 195.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

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Size

List Price (US$)

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PA007127

In Vivo Grade Recombinant Human IgG2 Isotype Control Antibody

1 mg

195.00

Description

The recombinant human IgG2 isotype control (hIgG2 kappa isotype control antibody) is produced in mammalian cells (HEK293 or CHO cells) like our other recombinant human IgG isotype control and mutant antibodies. The ideal recombinant human IgG2 isotype control has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG2 kappa constant regions. The human IgG2 kappa isotype control can be used in the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the hIgG2 isotype control with Avi-, His-, and DYKDDDDK-tags. A variety of conjugates (such as biotin, dyes, and fluorophores) with the human IgG2 isotype control are available.

PA007127: In vivo Grade Recombinant Human IgG2 Isotype Control Antibody (hIgG2 Isotype Control)

The in vivo grade recombinant human IgG2 isotype control antibody was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17.
Isotype: human IgG2, kappa.
Applications: an isotype-matched negative control for human IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The human IgG2 isotype control is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Naturally synthesized and secreted by plasma B cells, the immunoglobulin G (IgG) antibody is the most abundant antibody isotype found in blood and extracellular fluid. There are four IgG subclasses (IgG1, IgG2, IgG3, and IgG4) in humans, of which IgG1 is the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

The arms of the Y, also called the Fab (fragment, antigen-binding) region containing an identical antigen binding site, is composed of one variable (located at amino terminal end, VH and VL) and one constant domain (CH1 and CL) from each heavy and light chain of the antibody. VH and VL, also called the FV region, is the most important region for binding to antigens. Three variable loops of β-strands, also called the complementarity determining regions (CDRs or idiotypes), on VH and VL respecetively are responsible for binding to the antigen.

The base of the Y, also called the Fc (Fragment, crystallizable) region and composed of two heavy chains with two or three constant domains depending on the class of the antibody, binds to a specific class of Fc receptors and other immune molecules, such as complement proteins, to induce an appropriate immune response for a given antigen. The Fc region bears two highly conserved N-glycosylation sites, one on each heavy chain. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and α-2,6-linked sialic acid residues.

References of Human IgG2 Isotype Control Antibodies:

Impact of structural modifications of IgG antibodies on effector functions.
Baudino, L., et al. Front Immunol. 2023 Dec 11;14:1304365. doi: 10.3389/fimmu.2023.1304365. PMID: Not available
Immunoglobulin G (IgG) antibodies, the most abundant isotype in healthy human serum, are divided into four subclasses: IgG1 (60–70% in plasma), IgG2 (20–30%), IgG3 (5–8%), and IgG4 (1–3%). IgG antibodies are critical for neutralizing pathogens and other foreign substances in the adaptive immune system. Structural differences, such as disulfide bond linkages between heavy and light chains, distinguish the subclasses. Currently, only IgG1, IgG2, and IgG4 are considered for therapeutic purposes, but IgG3, the most potent in mediating Fc effector functions, and IgG polymorphisms could enhance antibody-mediated effector functions through differential binding to FcγRs and complement proteins. An example is the afucosylated humanized IgG1 antibody benralizumab (MEDI-563), which targets IL-5 Receptor α-chain for severe eosinophilic asthma.
Tags: activity of Human IgG2 isotype control; Human IgG2 isotype control

The solution structure of the human IgG2 subclass is distinct from those for human IgG1 and IgG4 providing an explanation for their discrete functions.
Lee, C. H., et al. J Biol Chem. 2019 Jul 19;294(29):11039-11052. doi: 10.1074/jbc.RA119.008305. PMID: 31110234
Human IgG2 antibody exhibits distinct therapeutic properties compared to IgG1, IgG3, and IgG4 subclasses. As the second most abundant IgG subclass, IgG2 binds to human FcγRII/FcγRIII but not to FcγRI or complement C1q. The solution structure of human myeloma IgG2 was determined using atomistic X-ray and neutron-scattering modeling. Analytical ultracentrifugation showed that IgG2 is monomeric with a sedimentation coefficient (s20, w0) of 7.2 S, with dimer formation ≤5% and independent of buffer conditions.
Tags: Human IgG2 isotype control antibody for animal model; Human IgG2 isotype control antibody for cancer research

Measuring of IgG2c isotype instead of IgG2a in immunized C57BL/6 mice with Plasmodium vivax TRAP as a subunit vaccine candidate in order to correct interpretation of Th1 versus Th2 immune response.
Hosseini-Safdar, M., et al. Exp Parasitol. 2020 Feb;209:107833. doi: 10.1016/j.exppara.2019.107833. PMID: 31838004
In C57BL/6 mice, evaluating IgG2c antibody alongside IFN-γ is critical for accurate interpretation of Th1 immune responses. Measuring murine isotype antibodies is essential in subunit vaccine development due to varied responses to recombinant proteins across inbred mouse strains with diverse genetic backgrounds. IFN-γ enhances antibody production, correlating with increased parasite-specific IgG2a levels. Since mouse IgG heavy chain constant regions are encoded by tightly linked genes inherited as Igh haplotypes, this study measured and compared IgG2a and IgG2c isotypes, their avidities, titration, and cytokines induced by recombinant PvTRAP antigen in C57BL/6 mice to clarify Th1/Th2 immune responses.
Tags: Human IgG2 isotype control antibody for mouse tumor model; Human IgG2 isotype control for flow cytometry

Whole-body immunoPET reveals active SIV dynamics in viremic and antiretroviral therapy–treated macaques.
Gangadhara, S., et al. Nat Methods. 2015 Aug;12(8):785-90. doi: 10.1038/nmeth.3320. PMID: 26053993
ImmunoPET/CT imaging using a labeled SIV-specific antibody identified sites of viral infection in SIV-infected macaques without requiring tissue biopsies, even when blood viral loads were below detection limits. A modified isotype control IgG antibody in a control macaque showed significantly lower background signal compared to infected animals, particularly in the liver, heart, and kidneys. Single-plane images and SUVmax quantification of modified mouse IgG in chronically infected and non-infected animals showed similar uptake, confirming the specificity of the SIV-specific antibody.
Tags: Human IgG2 isotype control in vivo; Human IgG2 isotype control dose

Neutralization Takes Precedence Over IgG or IgA Isotype-related Functions in Mucosal HIV-1 Antibody-mediated Protection.
Pegu, A., et al. EBioMedicine. 2017 Jan;15:114-123. doi: 10.1016/j.ebiom.2016.11.019. PMID: 27939044
In macaques, CH31 IgG1 and IgA2 isoforms infused before high-dose SHIV challenge were completely to partially protective, respectively, while non-neutralizing antibodies (CH54 IgG1 and CH38 mIgA2) were non-protective. IgG1 isotype broadly neutralizing antibodies (bnAbs) were more protective than IgA2 isotypes in mucosal models, attributed to greater neutralization activity. The study used a human ex vivo vaginal HIV-1 infection model and a rhesus macaque in vivo intrarectal SHIV challenge model to assess the protective capacity of functionally modified monoclonal bnAbs and non-neutralizing antibodies through isotype switching.
Tags: purified Human IgG2 isotype control; bulk Human IgG2 isotype control

For more references about human IgG2 isotype control antibodies please contact our scientific support team with message@sydlabs.com.

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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Human IgG2 Isotype Control Antibody

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References of Human IgG2 Isotype Control Antibodies: