Mouse IgG3 Isotype Control Antibody

Mouse IgG3 isotype control (natural and engineered mIgG3 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study.  

Please contact us to ask for a quote for the Fc silenced mouse IgG3 isotype control mutants, mIgG3 isotype control and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated mIgG3 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG3 isotype control and Fc-slient mutants are available.

PA007161: In Vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody (mIgG3 Isotype Control)

The in vivo grade recombinant mouse IgG3 isotype control antibody (mIgG3 isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG3, kappa.
Applications: an isotype-matched negative control for mouse IgG3 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant mouse IgG3 isotype control is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

References of Mouse IgG3 Isotype Control Antibody:

Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease.
Haferkamp, U., et al. Fluids Barriers CNS. 2023 Oct;20(1):78. doi: 10.1186/s12987-023-00471-y. PMID: 37907966
The following antibodies were used: Mouse IgG1 anti-human alanyl aminopeptidase, membrane (ANPEP/CD13) APC, Mouse IgG1 isotype control APC, Rabbit IgG anti-human platelet-derived growth factor receptor beta, Donkey IgG anti-rabbit Alexa Fluor 488, Mouse IgG2a anti-human excitatory amino acid transporter 1 PE, Mouse IgG2a isotype control PE, Human IgG1 anti-human protein thyrosin phosphatase receptor type C FITC, Human IgG1 isotype control FITC, Human IgG1 anti-human paired box 6 (PAX6) PE, Human IgG1 isotype control PE, Mouse IgG2a anti-human SRY-box transcription factor 2 (SOX2) Alexa Fluor 647, Mouse IgG3 isotype control Alexa Fluor 647, Human IgG1 anti-human SOX1 FITC, and Human IgG1 isotype control FITC. ...According to the World Health Organization, late-onset Alzheimer disease (LOAD) and other forms of dementia were the seventh leading cause of death worldwide in 2019, and the number of affected people is expected to continue to increase in the coming years. ...The pathophysiology of LOAD is poorly understood, which has contributed to a lack of preventive and curative treatments. ...The neurovascular unit (NVU), which consists of specialized brain capillary endothelial cells (BCECs) and other central nervous system (CNS) cells mainly carries out degradation and elimination of these toxic species. ...Animal models, in particular rodents, have been used to study the NVU in the context of LOAD but are limited in their ability to model this disease due to species-specific differences in brain anatomy and physiology.
Tags: activity of Mouse IgG3 isotype control; activity of Mouse IgG3 isotype control mAb

For more references about Mouse IgG3 Isotype Control Antibody please contact our scientific support team with message@sydlabs.com.

Questions and Answers:

Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage of the in vitro method. First, we do not use mouse ascites for antibody production. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies with the chemically defined medium. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations which is used in therapeutic antibody research. It is better to use the same isotype control mutation as the primary antibody in the application. 

Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate, if there is any, non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls against KLH, TNP, DNP,  GST, HEL, His-tag, DYKDDDDK, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit