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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The recombinant anti-human cd3 okt3 monoclonal antibody engineered by Syd Labs specifically targets the CD3 epsilon (CD3ε) chain of the T-cell receptor (TCR)-CD3 complex. While the OKT3 clone is historically renowned for its profound in vivo immunomodulatory mechanisms and TCR internalization properties, this specific product portfolio is rigorously optimized for high-resolution okt3 flow cytometry assays. Binding exclusively to mature T cells and medullary thymocytes, it serves as a critical analytical tool for evaluating CD3 surface expression, tracking receptor occupancy, and monitoring dynamic T-cell phenotypic shifts in ex vivo and in vitro flow cytometry applications.
To overcome the inherent limitations of traditional hybridoma-derived reagents in complex immunophenotyping panels, Syd Labs provides this recombinant flow cytometry matrix in highly specialized formats. For standard direct staining and robust isotype matching, we offer the native okt3 mouse igg2a (kappa) architecture. More importantly, to completely resolve the pervasive challenge of false-positive background noise caused by non-specific binding to Fcγ receptors on macrophages and monocytes, our engineered human igg1 fc silent okt3 flow cytometry format utilizes precise constant-domain mutations to deliver ultra-clean gating. Furthermore, for signal amplification in low-abundance target detection, our biotinylated okt3 antibody seamlessly integrates with streptavidin-fluorophore cascades, maximizing your panel design flexibility.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: OKT3 or OKT-3.
Isotype: Mouse IgG2a kappa.
Source: The anti-human CD3 monoclonal antibody (clone: OKT3) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: OKT3) specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: OKT3 or OKT-3.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-human CD3 monoclonal antibody (clone: OKT3) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant OKT3 antibody specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: OKT3 or OKT-3.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD3 monoclonal antibody (clone: OKT3) was produced in mammalian cells.
Conjugation: biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant OKT3 antibody specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
Biotin Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
FITC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
APC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
PE Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
Pacific Blue Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
PerCP Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
PE/Cy5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
PE/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
iFluor 488 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
iFluor 549 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
iFluor 594 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
iFluor 647 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
iFluor 700 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
APC/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
PerCP/Cy5.5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: OKT3)
Non-specific binding to Fcγ receptors on monocytes and macrophages often creates high background noise in CD3 staining. Our Human IgG1 Fc Silent OKT3 format is engineered to eliminate this interaction, resulting in cleaner populations and more accurate CD3 T-cell gating in complex PBMC samples.
While UCHT1 is the standard for routine surface staining, researchers often choose OKT3 flow cytometry when investigating TCR receptor occupancy, receptor internalization dynamics, or performing specific functional depletion studies. OKT3 is uniquely sensitive to the CD3ε chain conformation.
Yes. We provide recombinant okt3 antibody in various configurations, including biotinylated OKT3 for flexible signal amplification and direct-fluorophore conjugates. These are designed for seamless integration into multi-color panels for human T-cell immunophenotyping.
To ensure robust results, we recommend a titration-based workflow. By testing serial dilutions of your OKT3 antibody against a secondary fluorophore, you can identify the exact concentration where the fluorescence signal plateaus, ensuring consistent biomarker expression analysis in your flow cytometry experiments.
OKT3 is a potent T-cell stimulant. When using it for okt3 flow cytometry analysis, we recommend working at 4°C to minimize receptor internalization and metabolic activation. If your goal is T-cell expansion rather than detection, please refer to our dedicated Functional/In Vivo Grade OKT3 page.
Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry
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