MOPC-21 Isotype Control Antibodies

Background of MOPC-21 Isotype Control Antibodies

The MOPC-21 antibody (IgG1, kappa) has been used for a long time as an isotype-matched control antibody to estimate the degree of non-specific binding by an antigen-specific antibody. The variable regions of the MOPC-21 antibody share the sequences with the antibodies against all members of the dengue virus complex, and the Epstein-Barr virus gp350 protein. The recombinant MOPC-21 IgG1 kappa isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions.

As an ideal recombinant mouse IgG1 isotype control,  the recombinant MOPC-21 mIgG1 kappa isotype control has low or no specific binding to any mouse sample, and completely mouse variable regions and the mouse IgG1 kappa constant regions. The mouse IgG1 kappa isotype control and mutants can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

Please contact us to ask for a quote for the engineered MOPC-21 mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the MOPC-21 mouse IgG1 isotype control and mutants are available.

PA007155.m1: In Vivo Grade Recombinant MOPC-21 Mouse IgG1 Kappa Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant MOPC-21 mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: MOPC-21.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant MOPC-21 mouse IgG1 kappa isotype control antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of MOPC-21 Isotype Control Antibodies:

Programmed cell death ligand 2 regulates TH9 differentiation and induction of chronic airway hyperreactivity.
Kerzerho, J., et al. J Allergy Clin Immunol. 2013 Apr;131(4):1048-57, 1057.e1-2. doi: 10.1016/j.jaci.2012.09.027. PMID: 23174661
Differences between groups were analyzed by using the 2-tailed, unpaired Student t test and considered significant when the P value was less than .05. ...Asthma is defined as a chronic inflammatory disease of the airways; however, the underlying physiologic and immunologic processes are not fully understood. ...The aim of this study was to determine whether TH9 cells develop in vivo in a model of chronic airway hyperreactivity (AHR) and what factors control this development. ...We have developed a novel chronic allergen exposure model using the clinically relevant antigen Aspergillus fumigatus to determine the time kinetics of TH9 development in vivo. ...TH9 cells were detectable in the lungs after chronic allergen exposure.
Tags: activity of MOPC-21 isotype control; MOPC-21 isotype control

IL-17A and IL-2-expanded regulatory T cells cooperate to inhibit Th1-mediated rejection of MHC II disparate skin grafts.
Vokaer, B., et al. PLoS One. 2013 Oct 11;8(10):e76040. doi: 10.1371/journal.pone.0076040. PMID: 24146810
The neutralizing anti-IL-17A monoclonal antibody and the Isotype control antibody were intraperitoneally injected at a dose of 400 ?g twice a week after transplantation. ...Total RNA was extracted from skin grafts using the MagnaPure LC RNA Isolation Kit III for tissue (Roche Diagnostics). ...Number of mRNA copies was evaluated by using standard curve for each gene of interest and was normalized to ?-actin as housekeeping gene. ...Cell suspensions were incubated for 10 minutes with Fc block, stained for surface markers for 20 minutes, washed with Phosphate Buffer Saline/Bovine Serum Albumin 0.1%/NaN30.01%. ...For in vitro suppression assay, WT CD4+ T cells were isolated using CD4 negative isolation kit from Miltenyi, and used as responder cells.
Tags: MOPC-21 recombinant isotype control; MOPC-21 isotype control antibody for cancer research

Regulation of antinucleoprotein IgG by systemic vaccination and its effect on influenza virus clearance.
Lamere, M. W., et al. J Virol. 2011 May;85(10):5027-35. doi: 10.1128/JVI.00150-11. PMID: 21367900
Irrelevant isotype-matched control antibodies were purchased from BioXcell. ...Influenza virus-infected mice were sacrificed, and lungs were homogenized in PBS with penicillin-streptomycin. ...Statistical analysis was performed using Prism 5.0a software. ...To determine whether the 2009 pandemic H1N1 virus is susceptible to antibody-mediated NP immunity, we first vaccinated C57BL/6 and antibody-deficient, AID.?S mice with recombinant purified influenza virus NP, based on the PR8 amino acid sequence, as described previously. ...To determine how much NP was contained in the 2008/09 Fluzone preparation used prior to the 2009 H1N1 influenza virus pandemic, we performed a capture ELISA using a combination of monoclonal and polyclonal anti-NP antibodies and recombinant purified NP as a standard.
Tags: MOPC-21 isotype control antibody for mouse tumor model; MOPC-21 isotype control antibody in vivo

For more references about MOPC-21 Isotype Control Antibodies please contact our scientific support team with message@sydlabs.com.

In Vivo Grade Recombinant Isotype Control Antibodies (clone MOPC-21):
In vivo Grade Recombinant MOPC-21 Mouse IgG1 Isotype Control Antibody Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Mouse Fab Fragment
In vivo Grade Recombinant MOPC-21 Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human IgG2 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human IgG3 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Human Fab Fragment
In vivo Grade Recombinant MOPC-21 Human IgE Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Human IgM Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Human IgA1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Rat IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant MOPC-21 Rat Fab Fragment
In vivo Grade Recombinant MOPC-21 Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Rabbit IgG Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Ferret IgG1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Ferret IgM Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Ferret IgA Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG1 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG2 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG3 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Canine IgG4 Isotype Control Antibody
In vivo Grade Recombinant MOPC-21 Feline IgG1 Isotype Control Antibody

Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody