Name: Mouse IgG2b Fc Protein
Brand: Syd Labs
SKU: BP4121
Price: 500.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

BP4121

In Vivo Grade Recombinant Mouse IgG2b Fc Protein

1 mg

500.00

Description

Background

In vivo grade recombinant natural and engineered mouse IgG2b Fc proteins (mIgG2b Fc), and recombinant mouse IgG2b isotype control antibody and mutants are available. Biotinylated mIgG2b Fc, and mIgG2b Fc with Avi-, His-, and DYKDDDDK-tags are also available. Please contact us to ask for a quote for the engineered mouse IgG2b Fc proteins, biotinylated mIgG2b Fc, and mIgG2b Fc with Avi-, His-, and Flag-tags. The recombinant mouse IgG2b Fc protein and mutants can be used as the isotype control of the mIgG2b antibodies and the mIgG2b Fc fusion proteins in various in vitro and in vivo experiments.

BP4121: In Vivo Grade Recombinant Mouse IgG2b Fc protein (mIgG2b Fc)

The in vivo grade recombinant mouse IgG2b Fc protein (mIgG2b Fc, Glu 97 - Lys 335 of the constant region of the IgG2b heavy chain, Accession # P01867-2) was produced in mammalian cells and purified by protein A. The mIgG2b Fc protein includes the CH2 (126-225) and CH3 (234-330) domains of the heavy chain constant region as well as the hinge region (98-125) before the CH2 domain.
Molecular weight: Predicted 26.7 kDa. Due to glycosylation, the recombinant mouse IgG2b Fc protein migrates to 33-35 kDa based on the Bis-Tris PAGE result.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.

Shipping: The in vivo grade recombinant mouse IgG2b Fc protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of Mouse IgG2b Fc Protein:

Specificity of mouse and human Fcgamma receptors and their polymorphic variants for IgG subclasses of different species.
Wang, S., et al. Eur J Immunol. 2022 Jun;52(6):873-884. doi: 10.1002/eji.202149766. PMID: 35224703
Mouse IgG subclasses interact with mouse Fc? receptors (mFc?Rs), with IgG1 binding to mFc?RIIB and mFc?RIII, IgG2a and IgG2c binding to all mFc?Rs, IgG2b binding to mFc?RIIB, mFc?RIII, and mFc?RIV, and IgG3 binding to mFc?RI. Unexpectedly, mIgG3 complexes showed detectable binding to mFc?RIII in immune complex form and weak binding to mFc?RIV as Fab2C. Mouse IgG1 and IgG2b complexes bound predominantly to hFc?RIIAR131 and hFc?RIIAH131, weakly to hFc?RI and hFc?RIIB, while IgG2a and IgG2c bound to all hFc?Rs except hFc?RIIIB variants, and IgG3 bound to hFc?RI and weakly to hFc?RIIIAV158.
Tags: activity of Mouse IgG2b Fc Protein; Mouse IgG2b Fc Protein

Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding.
Overdijk, M. B., et al. Mol Immunol. 2020 Nov;127:88-96. doi: 10.1016/j.molimm.2020.08.009. PMID: 32889346
Mouse IgG subclasses cross-react with human Fc gamma receptors (hFc?Rs), showing substantial functional conservation despite interspecies differences. Binding of human IgG to mouse Fc?Rs has been used to study effector functions in mice, but mouse IgG binding to human Fc?Rs can cause misinterpretation in applications like immunostaining. This study documents that deglycosylation eliminates binding only for mouse IgG2b, highlighting its unique interaction profile with hFc?Rs.
Tags: Mouse IgG2b Fc Protein for animal model; Mouse IgG2b Fc Protein for cancer research

Relative Quantitation of Subclass-Specific Murine IgG Fc N-Glycoforms by Multiple Reaction Monitoring.
Li, Y., et al. ACS Omega. 2020 Jan 28;5(3):1415-1425. doi: 10.1021/acsomega.9b04412. PMID: 32001040
N-Linked glycosylation of the Fc domain of mouse IgG is a significant modulator of antibody functions and is subclass-specific. This study used multiple reaction monitoring to quantify subclass-specific peptides and Fc N-glycopeptides for IgG1*, IgG2b/2b*, IgG2a/2c, and IgG3 in mixed mouse serum, prepared by adding mouse IgG standard to C57BL/6 mouse serum. All peptides except IgG2aA were detected, aiding the study of how Fc glycosylation affects IgG subclass effector functions in various physiological or pathological states.
Tags: Mouse IgG2b Fc Protein for flow cytometry; Mouse IgG2b Fc Protein for mouse tumor model

Fc?RIV: A Novel FcR with Distinct IgG Subclass Specificity.
Tao, M. H., et al. Immunity. 2005 Aug;23(2):139-48. doi: 10.1016/j.immuni.2005.06.010. PMID: 16107918
Mouse IgG2a and IgG2b exhibit the greatest protective and pathogenic properties among mouse IgG subclasses, partly due to their binding to Fc?RIV, a novel ? chain-dependent activating Fc receptor. Fc?RIV, expressed on myeloid lineage cells, binds IgG2a and IgG2b with intermediate affinity but shows no binding to IgG1 or IgG3. Blocking Fc?RIV binding to pathogenic anti-platelet antibodies protected mice from antibody-induced thrombocytopenia.
Tags: Mouse IgG2b Fc Protein in vivo; Mouse IgG2b Fc Protein dose

Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose.
Ey, P. L., et al. Mol Immunol. 1978 Jun;15(6):563-7. doi: 10.1016/0161-5890(78)90070-6. PMID: 98765
A simple and rapid method for isolating pure mouse IgG1, IgG2a, and IgG2b immunoglobulins in nearly 100% yield is described. Mouse serum was fractionated on protein A-Sepharose 4B, with IgM, IgA, and IgE recovered in the effluent at pH 8.0. IgG subclasses were retained at pH 8.0 and sequentially eluted at pH 6.0?7.0 (IgG1), pH 4.5?5.0 (IgG2a), and pH 3.5?4.0 (IgG2b) using buffers of decreasing pH, provided the column's binding capacity was not exceeded.
Tags: function of Mouse IgG2b Fc Protein; in vivo study of Mouse IgG2b Fc Protein

For more references about mouse IgG2b fc protein please contact our scientific support team with message@sydlabs.com.

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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Mouse IgG2b Fc Protein

Mouse Fc protein (IgG2b), Mouse IgG2b Fc region, Recombinant mIgG2b Fc Protein

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BP4121: In Vivo Grade Recombinant Mouse IgG2b Fc protein (mIgG2b Fc)

References of Mouse IgG2b Fc Protein: