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Conditions of optimal biotinylated recombinant human PD-1/PDCD1 protein performance should be determined experimentally by the investigator. A promotion of 20% discount (coupon code PD1protein24A20) is effective until June 30, 2024.
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Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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BP004987: Biotinylated Human PD-1/PDCD1 Protein with the C-His-Avi Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The biotinylated recombinant human PD-1/PDCD1 protein with the His-Avi tag at the C-terminus has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity: The recombinant human PD-L1 with the C-hFc Tag captured on CM5 Chip via Protein A can bind to the biotinylated human PD-1 with the His-Avi Tag with an affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized biotinylated recombinant human PD-1/PDCD1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005587: Biotinylated Human PD-1/PDCD1 Protein with the C-hFc-Avi Tag
Source: The recombinant human PD-1/PDCD1 protein, the PD1 ECD (amino acids Leu25-Gln167) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The biotinylated recombinant human PD-1/PDCD1 protein with the hFc-Avi tag at the C-terminus has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the biotinylated recombinant human PD-1/PDCD1 protein with the hFc-Avi tag to the immobilized human PD-L1 with the mFc tag at 2 ug/ml (100 ul/Well) was 0.19 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized biotinylated recombinant human PD-1/PDCD1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant biotinylated human PD-1/PDCD1 protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
PD1 and PDL1 Antibodies:
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
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