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Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Our protein A ELISA kits for detection of leached Protein A are used in process development, process monitoring, and in final product testing to quantify natural and recombinant Protein A contamination in samples eluted off of protein A chromatography columns.
We offer two distinct Protein A detection assays, each kit with its own unique standard:
* one for the detection of natural and recombinant Protein A
* another for the detection of the Cytiva MabSelect SureTM ligand.
EK106-9000-1: Protein A ELISA kit for detection of natural and recombinant Protein A
* Sensitivity of 0.1 ug/ml (0.8 ppm)
* Accuracy/Recovery: 80-120%
* Precision: Inter-assay 10%, Intra-assay 15%
* Specificity for all variants of natural and recombinant Protein A
* 2 hour assay completion time
The Protein A ELISA kit is appropriate for processes that run columns that contain any Protein A resin.
A variety of pre-packed protein A affinity columns are available with NMab protein A resin, NMab Pro protein A resin, and UniMab 50 HC Protein A resin in a range of flexible column diameters and bed heights. The alkaline-stabilized recombinant protein A can be detected by our Protein A ELISA kit for detection of natural and recombinant Protein A.
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