Name: Llama IgG2b Fc Protein
Brand: Syd Labs
SKU: BP4119
Price: 500.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

BP4119

In Vivo Grade Recombinant Llama IgG2b Fc Protein

1 mg

500.00

Description

Background

In vivo grade recombinant natural and engineered llama IgG2b Fc proteins (lIgG2b Fc), and recombinant llama IgG2b isotype control antibody and mutants are available. Biotinylated lIgG2b Fc, and lIgG2b Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered llama IgG2b Fc proteins, biotinylated lIgG2b Fc, and lIgG2b Fc with Avi-, His-, and Flag-tags. The recombinant llama IgG2b Fc protein and mutants can be used as the isotype control of the lIgG2b antibodies and the lIgG2b Fc fusion proteins in various in vitro and in vivo experiments.

BP4119: In Vivo Grade Recombinant Llama IgG2b Fc protein (lIgG2b Fc)

The in vivo grade recombinant llama IgG2b Fc protein (lIgG2b Fc, Glu 1 - Lys 243 of the constant region of the IgG2a heavy chain, Accession # AAX73259.1) was produced in mammalian cells and purified by protein A. The lIgG2b Fc protein includes the CH2 (28-137) and CH3 (138-243) domains of the heavy chain constant region as well as the hinge region (1-27) before the CH2 domain.
Molecular weight: Predicted 27.3 kDa. Due to glycosylation, the recombinant llama IgG2b Fc protein migrates to 35-40 kDa based on the Bis-Tris PAGE result.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.

Shipping: The in vivo grade recombinant llama IgG2b Fc protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of Llama IgG2b Fc Protein:

Generation of nanobodies from transgenic ‘LamaMice’ lacking an endogenous immunoglobulin repertoire.
Drees, C., et al. Nat Commun. 2024 Jun 3;15(1):4567. doi: 10.1038/s41467-024-48735-x. PMID: 38830892
For the RBD nanobody discovery campaign, 15 ?g RBD-llama IgG2b Fc fusion protein was administered subcutaneously in 200 ?l SAS-emulsion, and 15 ?g antigen in 150 ?l PBS containing 50 ?g poly I:C was injected intraperitoneally. Mice received five boost immunizations at 2?4-week intervals. By inserting an engineered llama immunoglobulin heavy chain (IgH) locus into IgH-deficient mice, a transgenic mouse line, ‘LamaMouse,’ was generated to produce nanobodies with exceptional solubility and stability, offering a cost-effective alternative to llama-based nanobody production.
Tags: Llama IgG2b Fc Protein for animal model; Llama IgG2b Fc Protein for cancer research

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.
Dong, J., et al. Nature. 2021 Jul;594(7863):450-456. doi: 10.1038/s41586-021-03676-z. PMID: 34079120
Monomeric or trimeric nanobody sequences were fused to the Fc region of human IgG1 with a 6×His tag at the C-terminal end, or in some cases, a llama IgG2a hinge region was used instead, and cloned into the pVRC8400 vector. Trimeric nanobodies were connected via (GGGGS)×3 flexible linkers. Proteins were filtered through a 0.22-?m PVDF membrane before assays. For larger-scale production (0.2 to 1 L), nanobodies were purified using cOmplete His-tag purification resin and dialyzed in PBS.
Tags: Llama IgG2b Fc Protein for flow cytometry; Llama IgG2b Fc Protein for mouse tumor model

Fusion of the mouse IgG1 Fc domain to the VHH fragment (ARP1) enhances protection in a mouse model of rotavirus.
Gunaydin, G., et al. Sci Rep. 2016 Jul 21;6:30171. doi: 10.1038/srep30171. PMID: 27436087
A variable fragment of a heavy chain antibody (VHH), anti-rotavirus protein 1 (ARP1), was fused to the mouse IgG1 Fc to enhance protection against rotavirus-induced diarrhea in an infant mouse model. Co-expression of anti-rotavirus VHH fragments in Lactobacillus was developed with two expression cassettes in tandem. This fusion improved the protective capacity of ARP1 by inducing Fc-mediated effector functions, highlighting the potential of antibody-based drugs as alternatives to standard antifungals with high toxicity and failure rates.
Tags: Llama IgG2b Fc Protein in vivo; Llama IgG2b Fc Protein dose

Polyclonal alpaca antibodies protect against hantavirus pulmonary syndrome in a lethal Syrian hamster model.
Brocato, R. L., et al. Sci Rep. 2021 Sep 1;11(1):17592. doi: 10.1038/s41598-021-96884-6. PMID: 34471159
Polyclonal alpaca IgG antibodies (Alp-PcIgG-ANDV), including IgG1 and IgG3, were purified using the AKTA Pure Protein Purification system with a HiTrap Protein A column. The glycoprotein precursor coding region of ANDV Chile 9717869 was cloned into the pCAGGS plasmid, and protein expression was confirmed by Western blot using monoclonal antibodies targeting ANDV glycoproteins. The study suggests that increased DNA concentrations or additional time points may not significantly affect specific antibody yields.
Tags: function of Llama IgG2b Fc Protein; in vivo study of Llama IgG2b Fc Protein

An engineered human Fc domain that behaves like a pH-toggle switch for ultra-long circulation persistence.
Lee, C. H., et al. Nat Commun. 2019 Nov 26;10(1):5266. doi: 10.1038/s41467-019-13108-2. PMID: 31772162
The pharmacokinetic properties of antibodies are influenced by pH-dependent binding of the IgG Fc domain to the human neonatal Fc receptor (hFcRn). An engineered IgG1 Fc domain with enhanced hFcRn binding was developed, acting as a pH-toggle switch to extend circulation half-life. Biophysical studies elucidated the role of CH2?CH3 interface residues, His310, and His435 in pH-dependent binding. The engineered Fc domain improved pharmacokinetics in humanized knock-in mouse strains, relevant for antibody persistence studies.
Tags: Llama IgG2b Fc Protein mouse dose range; bulk Llama IgG2b Fc Protein

For more references about llama IgG2b fc protein please contact our scientific support team with message@sydlabs.com.

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Llama IgG2b Fc Protein

Llama Fc protein (IgG2b), Llama IgG2b Fc region, Recombinant lIgG2b Fc Protein

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BP4119: In Vivo Grade Recombinant Llama IgG2b Fc protein (lIgG2b Fc)

References of Llama IgG2b Fc Protein: