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Conditions of optimal biotinylated recombinant human PD-L1/B7-H1 protein performance should be determined experimentally by the investigator. A promotion of 20% discount (coupon code PDL1protein24A20) is effective until June 30, 2024.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005535: Biotinylated Human PD-L1/B7-H1 Protein with the C-His Tag
Source: The recombinant human PD-L1/B7-H1 protein, the PD-L1 ECD (amino acids Phe19-Arg238) fused with a His-tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The biotinylated recombinant human PD-L1/B7-H1 protein with the C-His tag has a predicted MW of 28.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity: The EC50 of the biotinylated human PD-L1/B7-H1 protein with the His tag to the immobilized anti-human PD-L1 antibody with the hFc tag at 0.5 ug/ml (100 ul/Well) was 9.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized biotinylated recombinant human PD-L1/B7-H1 protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The biotinylated recombinant human PD-L1/B7-H1 protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
PD1 and PDL1 Antibodies:
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
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