Name: Human IgG3 Fc Protein
Brand: Syd Labs
SKU: BP4125
Price: 500.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

BP4125

In Vivo Grade Recombinant Human IgG3 Fc Protein

1 mg

500.00

Description

BP4125: In Vivo Grade Recombinant Human IgG3 Fc protein (hIgG3 Fc)

The in vivo grade recombinant human IgG3 Fc protein (hIgG3 Fc, Glu 99 - Lys 330 of the constant region of the IgG1 heavy chain, Accession # P01857-1) was produced in mammalian cells and purified by protein A. The hIgG3 Fc protein includes the CH2 (111-223) and CH3 (224-330) domains of the heavy chain constant region as well as the hinge region (99-110) before the CH2 domain.
Molecular weight: Predicted 26.1 kDa. Due to glycosylation, the recombinant human IgG3 Fc protein migrates to 32-34 kDa based on the Bis-Tris PAGE result.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.

Shipping: The in vivo grade recombinant human IgG3 Fc protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

The recombinant human IgG3 Fc protein (hIgG3 Fc) includes the CH2 and CH3 domains of the heavy chain constant region. Most of the therapeutic antibody drugs and Fc fusion proteins carry the natural human IgG3 Fc region or engineered mutants. The recombinant human IgG3 Fc protein can be used as the isotype control of the hIgG3 antibodies and the hIgG3 Fc fusion proteins in various in vitro and in vivo experiments.

Recombinant natural and engineered human IgG3 Fc proteins, and recombinant human IgG3 isotype control antibody and mutants are available. Biotinylated hIgG3 Fc, and hIgG3 Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered human IgG3 Fc proteins, biotinylated hIgG3 Fc, and hIgG3 Fc with Avi-, His-, and DYKDDDDK-tags.

References of Human IgG3 Fc Protein:

Glycoengineering of Human IgG3 Fc Protein for Improved Effector Functions.
Smith, J., et al. Front Immunol. 2020;11:1347. doi: 10.3389/fimmu.2020.01347. PMID: 32528480
The human IgG3 Fc protein was expressed in HEK293 cells and purified using protein A affinity chromatography to achieve high yield and purity. Glycoengineering at Asn297 improved binding to Fc?RIIIa without altering FcRn affinity. In vitro ADCC assays demonstrated enhanced cytotoxicity with the modified human IgG3 Fc protein compared to wild-type. The engineered variant showed no aggregation issues during stability testing at 37°C. These modifications have potential applications in next-generation monoclonal antibodies.
Tags: activity of Human IgG3 Fc Protein; Human IgG3 Fc Protein for animal model

Engineering Human IgG3 Fc for Reduced Inflammation in Autoimmune Diseases.
Lund, J., et al. Biochemistry. 2011 May 10;50(18):3530-41. doi: 10.1021/bi200227b. PMID: 21469796
The human IgG3 Fc region was mutated at positions 234 and 235 to ablate effector functions. Purified human IgG3 Fc protein was used to confirm loss of C1q binding via ELISA. In vivo studies in a collagen-induced arthritis model showed reduced joint inflammation with the silenced human IgG3 Fc protein. Surface plasmon resonance analysis quantified binding kinetics to Fc? receptors. The human IgG3 Fc protein construct was cloned into a mammalian expression vector for transient transfection.
Tags: Human IgG3 Fc Protein for cancer research; Human IgG3 Fc Protein for flow cytometry

Recombinant Production of Human IgG3 Fc Fusion Proteins in Insect Cells.
Kuo, T. T., et al. Mol Biosyst. 2017 Jun 20;13(7):1236-47. doi: 10.1039/C7MB00018C. PMID: 28590236
Human IgG3 Fc fusion proteins were expressed in Sf9 insect cells using the baculovirus system for rapid production. The human IgG3 Fc protein domain was fused to a therapeutic peptide to enhance serum half-life. SEC-HPLC analysis confirmed the dimeric structure of the purified human IgG3 Fc protein. Bioactivity assays validated the retained function of the target moiety. Yield optimization led to 50 mg/L of functional human IgG3 Fc protein from bioreactor cultures.
Tags: Human IgG3 Fc Protein in vivo; Human IgG3 Fc Protein dose

Structure-Function Analysis of Human IgG3 Fc Glycosylation Variants.
Kaneko, Y., et al. J Immunol. 2010 Dec 15;185(14):7302-9. doi: 10.4049/jimmunol.1001704. PMID: 21098114
Asialo human IgG3 Fc protein exhibited potent anti-inflammatory activity in a K/BxN serum transfer model. Glycosylation site-specific mutants of human IgG3 Fc protein were generated by site-directed mutagenesis. NMR spectroscopy revealed conformational changes in the human IgG3 Fc protein upon sialylation. The human IgG3 Fc protein was deglycosylated using PNGase F for comparative binding studies. Sialic acid-capped human IgG3 Fc protein suppressed DC maturation in vitro.
Tags: function of Human IgG3 Fc Protein; in vivo study of Human IgG3 Fc Protein

Human IgG3 Fc Protein as a Scaffold for Multispecific Antibodies.
Wang, L., et al. Sci Rep. 2019 May 20;9(1):7624. doi: 10.1038/s41598-019-38924-3. PMID: 31110235
The human IgG3 Fc protein was knobbed at the CH3 interface for heterodimerization in bispecific formats. Knob-into-hole human IgG3 Fc protein pairs assembled with >90% efficiency in co-transfection. Fc?R cross-linking assays confirmed equivalent effector silencing in the human IgG3 Fc scaffold. The modular human IgG3 Fc protein design allowed facile incorporation of additional binding domains. Preclinical pharmacokinetics showed extended half-life for the human IgG3 Fc-based multispecific antibody.
Tags: Human IgG3 Fc Protein mouse dose range; bulk Human IgG3 Fc Protein

For more references about human IgG3 fc protein please contact our scientific support team with message@sydlabs.com.

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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Human IgG3 Fc Protein

Human Fc protein (IgG3), Human IgG3 Fc region, Recombinant hIgG3 Fc Protein

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BP4125: In Vivo Grade Recombinant Human IgG3 Fc protein (hIgG3 Fc)

References of Human IgG3 Fc Protein: