20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
PA000277-M20003: HA-Tag (26D11) Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: ELISA, IF, IP, WB;
Reactivity: Human and others;
Clone: 26D11;
Isotype: IgG1.BP002841-HCV-205: Recombinant Hepatitis C Virus NS4 Mosaic (HCV NS4 Mosaic) Protein
Source: E. coli-derived.
Purity > 95% pure as determined by 10% PAGE.
Specificity: Immunoreactive with sera of HCV-infected individuals.
Applications: ELISA, WB.BP000615-PKA-226: Recombinant Human Hexokinase-1
Source: E. coli-derived.
Specific activity is 7-8 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C.
Purity > 90%, by RP-HPLC and SDS-PAGE.PA002298-PA1503: MCL1 Polyclonal Antibody
Rabbit Polyclonal Antibody;
Applications: WB;
Reactivity: Human;
Isotype: Rabbit IgG.BP002694-PRO-1202: Recombinant Human Myeloid Cell Leukemia Sequence 1 (MCL1) protein
Source: E. coli-derived.
Purity: > 90% by SDS-PAGE.BP002924-PRO-1092: Recombinant human Oral cancer-overexpressed protein 1 (ORAOV1) Protein
Source: E. coli-derived.
Purity: >90.0% as determined by SDS-PAGE.PA000080-MA1060: P-Glycoprotein Monoclonal Antibody
Mouse Monoclonal Antibody;
Applications: WB, IHC-P, IHC-F, ICC;
Reactivity: Human;
Clone: PG-13;
Isotype: Mouse IgG1.MB111: Proteinase K Powder
The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale manufacturing of the recombinant enzyme has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of the recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: >99.5% by reducing SDS-PAGE. Each lot of the proteinase K powder was tested to ensure the absence of Nucleases and DNA. It can be used to make molecular biology grade proteinase K solution, PCR grade proteinase K solution, NGS grade proteinase K solution, CHIP grade proteinase K solution, and so on.
Specific activity: ≥ 34 units/mg of protein.
The lowest proteinase K price in the market.MB112: Proteinase K Solution, PCR Grade
Proteinase K is commonly used for protein digestion, DNA extraction, RNA purification, direct PCR, and genotyping. The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant enzyme production has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: Each lot of the recombinant proteinase K solution (molecular biology grade, PCR grade, NGS grade, chip grade, and RNA grade) was tested to ensure the absence of DNases and RNases, and free of DNA.
The cheapest proteinase K solution in the market.
Specific activity: ≥ 780 units/ml.