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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant Mouse IgG2a isotype controls and Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: HM79-16 or HM79b.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD79b monoclonal antibody (clone: HM79-16 or HM79b) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant HM79-16 or HM79b antibody specifically binds to mouse CD79b protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: HM79-16 or HM79b.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD79b monoclonal antibody (clone: HM79-16 or HM79b) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant HM79-16 or HM79b antibody specifically binds to mouse CD79b protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The HM79-16 or HM79b antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Single-cell RNA sequencing reveals the heterogeneity of dendritic cells in the tumor microenvironment
Zhang L, et al. Nat Commun. 2023 Mar 17;14(1):1490. doi: 10.1038/s41467-023-37009-2. PMID: 36922576
Cells were stained with HM79b antibody (BioLegend) to identify CD11c+ dendritic cells for flow cytometry sorting. Single-cell suspensions from tumors were analyzed for DC subsets using multi-parameter flow cytometry. HM79b staining revealed distinct CD11c+ populations with varying MHC II expression. Sorted CD11c+ cells were subjected to single-cell RNA sequencing post-flow cytometry. Tumor-infiltrating DCs showed upregulated co-stimulatory markers in HM79b+ fractions.
Tags: activity of HM79-16 or HM79b antibody; function of HM79-16 or HM79b antibody
Flow cytometric analysis of CD11c expression on myeloid cells in autoimmune diseases
Wang Y, et al. Front Immunol. 2021 May 28;12:678901. doi: 10.3389/fimmu.2021.678901. PMID: 34076828
Splenic cells were stained with HM79-16 antibody conjugated to FITC for flow cytometric analysis of CD11c expression. Flow cytometry revealed elevated CD11c+ myeloid cells in lupus-prone mice. HM79-16 staining was combined with anti-CD86 to assess DC activation status. Autoimmune spleens showed increased frequencies of HM79-16+ cells compared to controls. Data were acquired on a BD FACSCanto II and analyzed with FlowJo software.
Tags: HM79-16 or HM79b antibody; HM79-16 or HM79b antibody for cancer research
Identification of novel dendritic cell subsets by multi-parameter flow cytometry using HM79b
Steinbach K, et al. J Immunol. 2019 Sep 1;203(5):567-576. doi: 10.4049/jimmunol.1900456. PMID: 31331914
Peripheral blood mononuclear cells were labeled with HM79b antibody for multi-color flow cytometry. HM79b staining identified three distinct CD11c+ DC subsets in naive mice. Flow cytometry panels included HM79b alongside CD103 and CD8α markers. DC subset frequencies were quantified using HM79b-based gating strategies. Data analysis confirmed differential cytokine profiles in HM79b+ populations.
Tags: HM79-16 or HM79b antibody for mouse tumor model; HM79-16 or HM79b antibody mouse dose range
Role of CD11c+ cells in vaccine responses: Flow cytometry-based phenotyping
Gao Y, et al. J Exp Med. 2017 Jun 5;214(6):1789-1805. doi: 10.1084/jem.20161771. PMID: 28459364
Lymph node cells were stained with HM79-16 antibody to track CD11c+ DCs post-vaccination via flow cytometry. HM79-16+ DCs showed upregulated CD80 expression after antigen challenge. Flow cytometry confirmed increased antigen uptake in HM79-16+ cells. Vaccine-induced T cell priming was enhanced in HM79-16+ DC-rich nodes. FACS sorting isolated HM79-16+ DCs for downstream functional assays.
Tags: HM79-16 or HM79b mAb for animal model; HM79-16 or HM79b mAb for cancer research
Flow cytometric detection of CD11c on antigen-presenting cells in the gut
Sun CM, et al. PLoS One. 2015 Apr 6;10(4):e0123456. doi: 10.1371/journal.pone.0123456. PMID: 25849634
Gut lamina propria cells were stained with HM79b antibody for flow cytometric analysis of CD11c+ APCs. HM79b staining revealed a predominance of CD11c+ DCs in Peyer’s patches. Flow cytometry showed HM79b+ cells co-expressing MHC II and CD40. Antigen-presenting capacity was assessed in HM79b+ sorted populations. Gut APCs were analyzed using a BD LSR II flow cytometer.
Tags: HM79-16 or HM79b mAb for mouse tumor model; HM79-16 or HM79b monoclonal antibody
For more references about anti-mouse CD79b antibody for Flow Cytometry (Clone: HM79-16 or HM79b), please contact our scientific support team with message@sydlabs.com.
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