Anti-Mouse CD11c Monoclonal Antibodies

Background of anti-mouse CD11c antibody

The hamster anti-mouse CD11c monoclonal antibody N418 (hamster IgG2 kappa) reacts with the mouse CD11c protein, the most widely used defining marker for dendritic cells (DCs).  The CD11c protein helps cells stick together and is involved in many processes, including phagocytosis, cell migration, and the activation of T cells. CD11c helps cells stick together by binding to molecules like ICAM-1, fibrinogen, and iC3b, helps cells engulf and digest other cells, helps Langerhans cells activate T cells, regulates neutrophil maturation and function, and helps dendritic cells capture and activate other cells. In addition to dendritic cells and tissue macrophages, CD11c is expressed in different B-cell lymphomas, including chronic lymphocytic leukemia. CD11c-expressing cells are also found in the mouse nervous system.

Our recombinant N418 antibodies have a part (variable regions) or complete amino acid sequences of the hamster anti-mouse CD11c monoclonal antibody (hybridoma clone name or number: N418).

PA007390.m1: In vivo Grade Recombinant Anti-mouse CD11c Monoclonal Antibody, Mouse IgG1 Kappa (Clone: N418)

The in vivo grade recombinant anti-mouse CD11c mouse IgG1 monoclonal antibody was produced in mammalian cells. 
Clone: N418.
Isotype: Mouse IgG1 Kappa.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: N418) specifically binds to mouse CD11c.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11c protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007390.hs2: In vivo Grade Recombinant Anti-mouse CD11c Monoclonal Antibody, Hamster IgG2 Kappa (Clone: N418)

The in vivo grade recombinant anti-mouse CD11c hamster IgG2 monoclonal antibody was produced in mammalian cells. 
Clone: N418.
Isotype: Hamster IgG2 kappa.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: N418) specifically binds to mouse CD11c.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD11c protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse CD11c antibodies (clone of N418) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse CD11c Monoclonal Antibody:

CD11c-specific bio-nanocapsule enhances vaccine immunogenicity by targeting immune cells.
Matsuo, H. I., et al. J Nanobiotechnology. 2018 Aug 4;16(1):59. doi: 10.1186/s12951-018-0386-6. PMID: 30077180
We have identified that anti-CD11c IgGs (clone N418)-displaying ZZ-BNC (α-DC-ZZ-BNC) could accumulate into splenic DCs in mice through intravenous (IV) injection. ...ZZ-BNC was conjugated with anti-CD11c IgGs and model Ags OVA. ...As shown in Fig. 3a, α-DC-ZZ-BNC complex could target approximately 12% of CD11c+ cells (i.e., DCs) in ILNs more efficiently than ZZ-BNC, IgG-ZZ-BNC, BNC, and 40-nm beads after SC injection. ...On the other hand, α-DC-ZZ-BNC complex injected IM could target approximately 9% of CD11c+ cells in PLN, which was higher than ZZ-BNC, IgG-ZZ-BNC, BNCs, and 40-nm fluorescent beads (Fig. 3c). ...Either anti-CD11c IgG or Armenian hamster IgG isotype control was displayed onto ZZ-BNC-OVA as described previously (Additional file 1).
Tags: anti-mouse CD11c N418 in vivo; anti-mouse CD11c N418 antibody in vivo

Targeting viral antigens to CD11c on dendritic cells induces retrovirus-specific T cell responses.
Ejaz, A., et al. PLoS One. 2012;7(9):e45102. doi: 10.1371/journal.pone.0045102. PMID: 23028784
Furthermore, recent evidence has shown that C3-fragments on the surface of retroviruses like HIV and Friend virus (FV) enhance infection of DCs most likely through CD11c and CD11b binding, which subsequently leads to an improved virus-specific CD8+ T cell activation by DCs. ...CD11c is the α-chain of CR4 (CD11c/CD18). ...In mice, CD11c is preferentially expressed on myeloid DCs, including both CD8+ and CD8 - subpopulations and is often used as DC-specific marker. ...In experimental tumor models, targeting antigens to CD11c by specific Abs conjugated to Ags, single-chain Ab fragments (scFv) recombinantly fused to Ags or liposomes incorporating Ags have been demonstrated to successfully induce specific immune responses. ...Thus far targeting Ags to CD11c has merely been tested in tumor models.
Tags: anti-mouse CD11c N418 antibody in animal model; anti-mouse CD11c N418 mAb in animal model

Engineered hepatitis B virus surface antigen L protein particles for in vivo active targeting of splenic dendritic cells.
Matsuo, H. I., et al. Int J Nanomedicine. 2012;7:3341-50. doi: 10.2147/IJN.S32813. PMID: 22848163
ZZ-BNCs displaying anti-CD11c monoclonal antibodies (α-CD11c-ZZ-BNCs) were found to accumulate with approximately 62% of splenic DCs, and reside within some of the them. ...After the fusion with liposomes containing antigens, the α-CD11c-ZZ-BNCs could elicit the respective antibodies more efficiently than other nontargeting control vaccines, suggesting that this DC-specific nanocarrier is promising for future vaccines. ...Recently, DC-targeting nanocarriers have been developed for effective vaccines using the DC-specific delivery of Ag-encoding genes or exogenous antigens. ...Armenian hamster monoclonal α-CD11c IgG (clone N418), rat α-major histocompatibility complex (MHC) class II IgG2b (clone NIMR-4), and Armenian hamster IgG isotype control were purchased from eBioscience (San Diego, CA). ...However, these vectors present the potential problems commonly observed in viral vectors, including unexpected immunological responses in patients, accidental disturbance of the patients’ chromosomes, and unacceptability for Ags other than DNA.
Tags: anti-mouse CD11c N418 antibody in vivo; anti-mouse CD11c N418 in mouse tumor model

Targeting dendritic cells with antigen-containing liposomes: a highly effective procedure for induction of antitumor immunity and for tumor immunotherapy.
van Broekhoven, C. L., et al. Cancer Res. 2004 Jun 15;64(12):4357-65. doi: 10.1158/0008-5472.CAN-04-0138. PMID: 15205352
A novel metal-chelating lipid, 3(nitrilotriacetic acid)-ditetradecylamine, was incorporated into B16-OVA-derived PMV, allowing recombinant hexahistidine-tagged forms of single chain antibody fragments to the DC surface molecules CD11c and DEC-205, to be conveniently “engrafted” onto the vesicle surface by metal-chelating linkage. ...Antigen is internalized via a number of surface receptors, including the complement receptor CD11c/CD18 (8, 9, 10) and the endocytic receptor DEC-205 (11, 12). ...The receptors CD11c/CD18 and DEC-205 are believed to play a crucial role in the process of antigen capture and presentation and are expressed almost exclusively on DCs. ...Recombinant ScFv Abs N418 (anti-CD11c) and NLDC145 (anti-DEC-205; Refs. 25, 26) were developed using the V H and V L regions amplified from cDNA from the respective hybridomas and shown to bind to murine DCs. ...A hexahistidine (6H) tag was incorporated at the COOH terminal of the Abs, CD11c-ScFv and DEC-205-ScFv, respectively, and these were expressed in the baculovirus expression system and purified as described previously (27).
Tags: function of anti-mouse CD11c N418 mAb; bioactivity of anti-mouse CD11c N418 mAb

For more references about Anti-mouse CD11c Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.