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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The rat anti-mouse CD8a monoclonal antibody clone YTS 105.18 specifically reacts with the mouse CD8a protein?a T-cell surface glycoprotein encoded by the CD8A gene. While some suppliers and literature misidentify this clone as a rat IgG2a monoclonal antibody, heavy chain sequence validation strictly confirms that its true isotype is rat IgG2b kappa. Beyond its physiological role as a diagnostic and prognostic marker in therapeutic research, clone YTS 105.18 is widely recognized as a premium, non-depleting anti-mouse CD8a reagent, distinguishing it functionally from other clearing clones like 2.43 or YTS 169.4.
Our in vivo grade recombinant anti-mouse CD8a monoclonal antibody (clone YTS 105.18) is engineered using a mammalian expression system to ensure pristine sequence fidelity and exceptional batch-to-batch consistency. Because the YTS 105.18 antibody blocks CD8+ T-cell activity without triggering in vivo cell death, it serves as the ideal tool for CD8+ T cell blockade, xenograft transplantation tolerance induction, and autoimmune studies (such as modulating insulin-dependent diabetes mellitus in NOD mice models). Formulated via our specialized low endotoxin platform, this recombinant formulation delivers the reliability required for artifact-free functional assays and publication-ready datasets.
The in vivo grade recombinant anti-mouse CD8a rat IgG2b monoclonal antibody was produced in mammalian cells.
Clone: YTS 105.18.
Isotype: Rat IgG2b kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YTS 105.18) specifically binds to the mouse CD8a.
Applications: Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD8a antibodies (clone of YTS 105.18) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
No, clone YTS 105.18 is a well-documented non-depleting anti-mouse CD8a antibody. Unlike clone 2.43 or YTS 169.4, which actively clear CD8+ T cells in vivo, YTS 105.18 blocks CD8+ T-cell receptor engagement and functional activity without inducing cell death. It is the premier choice for CD8+ T cell blockade and immunomodulation assays where cell viability must be preserved.
Syd Labs' recombinant YTS 105.18 antibody is strictly validated as a rat IgG2b kappa isotype. Although some historical literature and alternative suppliers misclassify the YTS 105.18 clone as a rat IgG2a, our heavy-chain vector sequence definition definitively confirms its rat IgG2b lineage. For control experiments, we recommend pairing it with a purified rat IgG2b isotype control.
Syd Labs’ in vivo grade recombinant anti-mouse CD8a clone YTS 105.18 is highly optimized for functional assays that require T-cell inhibition without depletion. Key applications include inducing xenograft transplantation tolerance, evaluating autoimmune modulation (such as diabetes prevention protocols in NOD mice), and performing high-precision Flow Cytometry (FC) or Immunohistochemistry (IHC) on paraffin sections.
Yes, our recombinant YTS 105.18 is manufactured through an advanced mammalian cell expression system and processed via a rigorous low endotoxin platform (<1 EU/mg). The formulation is completely azide-free and carrier-free in 1x PBS, preventing non-specific cellular toxicity and ensuring artifact-free data in delicate animal modeling experiments.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant rat IgG2b isotype control antibody
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)
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