Name: Anti-Human CD3 Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007196
Price: 250.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007196

In vivo Grade Recombinant Anti-human CD3E Monoclonal Antibody (Clone: SP34-2), Mouse IgG1 Lambda

200 ug

250.00

PA007196

In vivo Grade Recombinant Anti-human CD3E Monoclonal Antibody (Clone: SP34-2), Mouse IgG1 Lambda

1 mg

500.00

Description

PA007196: In vivo Grade Recombinant Anti-human CD3E Mouse IgG1 Lambda Monoclonal Antibody (Clone: SP34-2)

Recombinant mouse IgG1 Monoclonal Antibody.
Clone: SP34-2.
Isotype: Mouse IgG1 lambda.
Source: The anti-human CD3 monoclonal antibody (clone: SP34-2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: SP34-2) specifically binds to the human T cell receptor CD3.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD3 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The anti-human CD3 antibody (clone: SP34-2), a derivative of SP34, is known to cross-react with a major subset of peripheral blood lymphocytes in both human and non-human primates, including baboon, rhesus, cynomolgus, and pigtail macaque monkeys. The SP34-2 antibody specifically recognizes a major subset of CD3-positive cells from non-human primates, similar to its human counterpart, but does not react with monocytes or granulocytes.

The SP34-2 antibody is used in flow cytometry to identify and analyze CD3-positive cells in human and various non-human primate species. It can also be used to stimulate cell proliferation in various primate peripheral blood mononuclear cells (PBMC).

References of anti-human CD3 antibody (SP34-2):

Substitutions in Nef That Uncouple Tetherin and SERINC5 Antagonism in Simian Immunodeficiency Virus SIVagm
Rahmberg D, et al. J Virol. 2022 Jun 8;96(11):e0017622. doi: 10.1128/jvi.00176-22. Epub 2022 May 19. PMID: 35612512
This study investigates the role of Nef in Simian Immunodeficiency Virus (SIVagm) in African green monkeys (AGMs), which exhibit low immune activation despite high viremia. Flow cytometry was used to analyze cells stained with LIVE/DEAD Fixable Aqua, anti-human CD3 (SP34-2), CD4 (L200), CD8 (SK1), and CD20 (L27) antibodies. Virus stocks were generated by transfecting 293T cells with plasmid DNA encoding full-length SIVagm proviruses. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using Ficoll gradient centrifugation, and viral RNA was extracted from plasma using the QIAamp Viral RNA minikit.
Tags: anti-human CD3 SP34-2; anti-human CD3 (SP34-2) in vivo antibody

Autologous humanized mouse models of iPSC-derived tumors enable selection of immunotherapy candidates
Scarpa M, et al. Cell Rep Methods. 2022 Jan 24;2(1):100154. doi: 10.1016/j.crmeth.2021.100154. PMID: 35043006
This study explores autologous humanized mouse models generated from human induced pluripotent stem cells (iPSCs) to evaluate immunotherapy candidates. Single-cell suspensions were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain Kit and anti-human antibodies: CD45 (HI30), CD3 (SP34-2), CD4 (OKT4), CD8 (SK), and PD-1 (EH12.2H7). Human iPSCs were derived from peripheral blood mononuclear cells (PBMCs) using Sendai virus-based reprogramming. Tumor growth was monitored via caliper measurements and bioluminescence imaging, with immune cell infiltration analyzed by flow cytometry in tumor, spleen, and bone marrow samples.
Tags: anti-human CD3 SP34-2 antibody; anti-human CD3 antibody (clone SP34-2)

Development and preliminary clinical activity of MGD019, a bispecific PD-1 x CTLA-4 dual-affinity retargeting (DART?) molecule
Horowitz JM, et al. Cell Rep Med. 2020 Dec 22;1(9):100153. doi: 10.1016/j.xcrm.2020.100153. PMID: 33361387
This study describes the development and preliminary clinical activity of MGD019, a bispecific molecule targeting PD-1 and CTLA-4. Flow cytometry was conducted using anti-CD3 (SP34-2), anti-CD4 (L200), and anti-CD8 (RPA-T8) antibodies. Cynomolgus monkeys received MGD019 at doses of 10 to 100 mg/kg weekly for 4 weeks. Peripheral blood samples were collected for pharmacokinetic analysis, and immune cell populations were quantified in spleen and lymph nodes at necropsy. Binding to PD-1 and CTLA-4 was confirmed using surface plasmon resonance.
Tags: anti-human CD3 SP34-2 in animal model; anti-human CD3 antibody (clone SP34-2)

Modified Vaccinia Virus Ankara Encoding Influenza Virus Hemagglutinin Induces Heterosubtypic Immunity in Mice
Florek AM, et al. Vaccine. 2014 Nov 12;32(48):6394-400. doi: 10.1016/j.vaccine.2014.08.036. Epub 2014 Sep 2. PMID: 25183737
This study evaluates a Modified Vaccinia Virus Ankara (MVA) vaccine encoding influenza virus hemagglutinin in mice. Splenocytes were stained with anti-CD3 (SP34-2), anti-CD4 (L200), and anti-CD8 (SK1) antibodies for flow cytometric analysis. Mice were vaccinated intramuscularly with 10^7 PFU of MVA-HA or control MVA, followed by an influenza virus challenge with 10^5 TCID50 of H1N1 three weeks later. Lung viral titers were measured by plaque assay, and IFN-? production by T cells was assessed via ELISPOT assay.
Tags: anti-human CD3 SP34-2 in cancer research; anti-human CD3 antibody SP34-2

CD8+ cellular immunity mediates rAd5 vaccine protection against Ebola virus infection of nonhuman primates
Sullivan NJ, et al. Nat Med. 2011 Sep 4;17(9):1128-31. doi: 10.1038/nm.2447. PMID: 21880723
This study examines the role of CD8+ cellular immunity in rAd5 vaccine protection against Ebola virus in cynomolgus macaques. Lymphocytes were stained with anti-CD3 (SP34-2), anti-CD8 (SK1), and anti-IFN-? (B27) antibodies for intracellular cytokine staining. Macaques were vaccinated with rAd5 expressing Ebola virus glycoprotein and challenged with 1000 PFU of Ebola virus 4 weeks later. Peripheral blood was collected weekly for immune response monitoring via flow cytometry, with survival tracked daily and necropsy performed on nonsurvivors.
Tags: anti-human CD3 SP34-2 in human tumor model; clone SP34-2 of human CD3 antibody

For more references about anti-human CD3 antibody (SP34-2), please contact our scientific support team with message@sydlabs.com.