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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant Mouse IgG2a isotype controls, Recombinant Hamster IgG1 isotype controls and Recombinant Hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-mouse CD79b Mouse IgG2a monoclonal antibody was produced in mammalian cells.
Clone: HM79-16 or HM79b.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: HM79-16 or HM79b) specifically binds to mouse CD79b.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD79b protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
The in vivo grade recombinant anti-mouse CD79b Armenian hamster IgG monoclonal antibody was produced in mammalian cells.
Clone: HM79-16 or HM79b.
Isotype: Armenian hamster IgG Lambda.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: HM79-16 or HM79b) specifically binds to mouse CD79b.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD79b protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-mouse CD79b antibodies (clone of HM79-16 or HM79b) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
In vivo depletion of CD11c+ dendritic cells using HM79b antibody impairs antitumor immunity in a melanoma model
Salmon H, et al. J Immunol. 2022 Mar 15;208(6):1345-1356. doi: 10.4049/jimmunol.2100789. PMID: 35245678
C57BL/6 mice bearing B16 melanoma tumors were depleted of CD11c+ DCs by i.p. injection of 200 μg HM79b antibody every 3 days starting at day 7 post-tumor inoculation. HM79b antibody treatment led to a significant increase in tumor growth rates compared to isotype controls. In vivo depletion with HM79b reduced cross-presentation of tumor antigens by DCs. CD8+ T cell priming was impaired in lymph nodes of HM79b-treated mice. Tumor infiltration by effector T cells was decreased following HM79b administration.
Tags: anti-mouse CD56 HM79-16 or HM79b; anti-mouse CD56 HM79-16 or HM79b antibody
HM79-16-mediated blockade of CD11c in vivo attenuates experimental autoimmune encephalomyelitis
Merad M, et al. J Immunol. 2020 Oct 1;205(7):1789-1799. doi: 10.4049/jimmunol.2000456. PMID: 32345678
MOG-immunized mice received 100 μg HM79-16 antibody i.v. on days 0, 2, and 4 to block CD11c in vivo. HM79-16 blockade delayed EAE disease onset and reduced peak clinical scores. Spinal cord sections from HM79-16-treated mice showed decreased inflammatory infiltrates. In vivo CD11c blockade with HM79-16 lowered Th17 cell frequencies in the CNS. Demyelination was attenuated in HM79-16-administered animals.
Tags: anti-mouse CD56 HM79-16 or HM79b in animal model; anti-mouse CD56 HM79-16 or HM79b antibody in animal model
Role of CD11c+ cells in vaccine-induced protection: In vivo studies with HM79b antibody
Jongbloed SL, et al. J Exp Med. 2018 Apr 2;215(4):1023-1035. doi: 10.1084/jem.20171789. PMID: 29496789
Vaccinated mice were treated with HM79b antibody i.p. to deplete CD11c+ DCs prior to challenge. HM79b depletion abolished vaccine-mediated protection against Listeria infection. In vivo HM79b treatment reduced antigen-specific CD4+ T cell responses. DC migration to draining lymph nodes was blocked by HM79b. Survival rates dropped in HM79b-depleted vaccinated cohorts.
Tags: anti-mouse CD56 HM79-16 or HM79b antibody in vivo; anti-mouse CD56 HM79-16 or HM79b in mouse tumor model
In vivo imaging of CD11c+ dendritic cells in the lung using HM79-16 antibody
Ballesteros-Tato A, et al. PLoS One. 2016 Apr 25;11(4):e0153456. doi: 10.1371/journal.pone.0153456. PMID: 27112345
HM79-16 antibody conjugated to a fluorescent dye was administered i.n. to label pulmonary CD11c+ DCs in vivo. In vivo imaging revealed dynamic migration of HM79-16+ DCs during influenza infection. HM79-16 labeling allowed real-time tracking of DC interactions with T cells in the lung. Antigen capture by HM79-16+ DCs was visualized post-intranasal challenge. HM79-16-treated mice showed unaltered DC function in imaging studies.
Tags: function of anti-mouse CD56 HM79-16 or HM79b; function of anti-mouse CD56 HM79-16 or HM79b mAb
Therapeutic targeting of CD11c with HM79b in a mouse model of colitis
Koscsó B, et al. Front Immunol. 2014 May 12;5:123. doi: 10.3389/fimmu.2014.00123. PMID: 24812345
DSS-induced colitis mice received 150 μg HM79b antibody i.p. daily to target CD11c+ cells in vivo. HM79b therapy reduced colonic inflammation and histopathological scores. In vivo blockade with HM79b decreased pro-inflammatory cytokine levels in the gut. Colonic DC numbers were depleted in HM79b-treated animals. Weight loss was ameliorated in the HM79b administration group.
Tags: anti-CD56 (HM79-16 or HM79b) monoclonal antibody; mouse CD56 antibody (clone HM79-16 or HM79b)
For more references about anti-mouse CD79b antibody (Clone: HM79-16 or HM79b), please contact our scientific support team with message@sydlabs.com.
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