Anti-Mouse Ter-119 Monoclonal Antibodies

Background of Anti-Mouse Ter-119 Antibody

Our recombinant TER-119 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse Ter-119 monoclonal antibody (hybridoma clone name or number: TER-119).

PA007526.r2b: In Vivo Grade Recombinant Anti-Mouse Ter-119 Monoclonal Antibody (Clone: TER-119), Rat IgG2b Kappa

The in vivo grade recombinant anti-mouse Ter-119 rat IgG2b monoclonal antibody was produced in mammalian cells.
Specificity: Ter-119.
Clone: TER-119.
Isotype: rat IgG2b, kappa.
Applications: Western blot, immunoprecipitation (IP), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-mouse Ter-119 antibodies (clone of TER-119) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse Ter-119 Monoclonal Antibody:

Affinity-Restricted Memory B Cells Dominate Recall Responses to Heterologous Flaviviruses.
Wong, R., et al. Immunity. 2020 Nov 17;53(5):1078-1094.e7. doi: 10.1016/j.immuni.2020.09.001. PMID: 33010224
The following antibodies were purchased from Bio X Cell: M1/70 (CD11b), 2C11 (CD3), GK1.5 (CD4), 53?6.7 (CD8), and TER119. ... Splenocytes were stained with rat anti-CD4, CD8, CD11b, CD3, Ter119, IgM, and IgD on ice for 20 min, wash, and then incubated on plates pre-coated with 1 ug/mL of mouse anti-rat IgG (3053-01, Southern Biotech) for 20 min at 4°C. ... The non-adherent cell fraction was collected, washed, and then stained with MBC and GC B cell surface markers (CD19, GL7, CD38, CD80, CCR6 and DIII tetramers). ... Antigen-specific bone marrow plasma cells were subjected to CD138 enrichment prior to DIII-tetramer staining. ... Total bone marrow cells were stained with anti-CD138 APC, cells washed and then stained with anti-PE magnetic beads (0.5 uL/107 cells, Miltenyi Biotec).
Tags: anti-mouse Ter-119 TER-119; anti-mouse Ter-119 TER-119 mAb

Coordinated Viral Control by Cytotoxic Lymphocytes Ensures Optimal Adaptive NK Cell Responses.
Diaz-Salazar, C., et al. Cell Rep. 2020 Sep 22;32(12):108186. doi: 10.1016/j.celrep.2020.108186. PMID: 32966792
InVivoMab Anti-Mouse Ter-119 (NK cell enrichment, clone TER-119) Bio X Cell Cat#BE0183; RRID:AB_10949625 ... Splenic NK cells and/or CD8+ T cells were enriched by negative selection using BioMag? Goat Anti-Rag IgG (QIAGEN), mixed with the following Antibody cocktail (?CD4 (GK1.5 clone, BioXcell), ?CD19 (1D3 clone, BioXcell), ?Ly6G (1A8 clone, BioXcell)), and then sorted to > 95% purity on a BD FACSAria II cell sorter. ... Depletion of CD8+ T cells and other lymphocyte populations was achieved by intraperitoneal injection of the following purified antibodies: ?CD4 (GK1.5 clone, BioXcell, 200 ?g/mouse), ?CD8 (2.43 clone, BioXcell, 100 ?g/mouse), or isotype control (LTF-2 clone, BioXcell, 100 ?g/mouse) one day prior to infection. ... InVivoMab Anti-Mouse CD19 (NK cell enrichment, clone 1D3) Bio X Cell Cat#BE0150; RRID:AB_10949187 ... InVivoMab Anti-Mouse Ter-119 (NK cell enrichment, clone TER-119) Bio X Cell Cat#BE0183; RRID:AB_10949625
Tags: anti-mouse Ter-119 TER-119 in animal model; anti-mouse Ter-119 TER-119 antibody in animal model

Cytomegalovirus Infection Drives Avidity Selection of Natural Killer Cells.
Adams, N. M., et al. Immunity. 2019 Jun 18;50(6):1381-1390.e5. doi: 10.1016/j.immuni.2019.04.009. PMID: 31103381
NK cells (TCR? ?CD3? ?CD19 ?F4/80 ?CD45+NK1.1+) were enriched from spleens of pooled C57BL/6 mice by negative selection over BioMag goat anti-rat IgG beads (Qiagen) coated with rat anti-mouse CD8?, CD4, CD19, and Ter-119 antibodies (Bio X Cell, clones 2.43, GK1.5, 1D3, and TER-119 respectively) before being sorted to high purity on an Aria II cytometer (BD Biosciences). ...Purified splenic NK cells with different Ly49H expression were loaded with 5 ?g/mL Fura-2AM for 30 min and transferred into colorless RPMI (no phenol red) containing 5% FCS. 8 × 104 NK cells were mixed 1:4 with Ba/F3-m157 cells and imaged in 8-well chamber slides (Thermo Fisher Scientific) using an inverted fluorescence video microscope (IX-81; Olympus) fitted with a 20 ×, 0.75 NA objective lens (Olympus). ...Ly49Hlo and Ly49Hhi NK cells were sorted from the spleens of WT mice 1.5 days post MCMV infection. ...Paired-end reads were trimmed for adaptors and removal of low-quality reads using Trimmomatic (v.0.36) (Bolger et al., 2014). ...For graphs, data are shown as mean ± SEM, and unless otherwise indicated, statistical differences were evaluated using an unpaired, two-tailed Student’s t test, assuming equal sample variance.
Tags: anti-mouse Ter-119 TER-119 in cancer research; anti-mouse Ter-119 TER-119 mAb in cancer research

ADAM17 limits the expression of CSF1R on murine hematopoietic progenitors.
Becker, A. M., et al. Exp Hematol. 2015 Jan;43(1):44-52.e1-3. doi: 10.1016/j.exphem.2014.10.001. PMID: 25308957
ALPs express low levels of CSF1R and differentiate into monocytes in a dose-dependent manner with exogenous M-CSF administration. ... CSF1R surface expression was low on ALPs, similar to that seen in LMPPs and markedly less than that observed in CMPs. ... Fate decisions during hematopoietic differentiation are regulated by complex interactions between cell-extrinsic and cell-intrinsic cues. At certain intermediates, progenitor cell differentiation in vivo is often more restricted than their epigenetic profiles or in vitro potentials might predict [2,11]. ... Indeed, ALPs, along with granulocyte macrophage progenitors, CMPs, and LMPPs, all showed elevated expression of surface CSF1R (Fig. 2; gating strategies shown in Supplementary Figure E1, online only, available at www.exphem.org). ... Unexpectedly, ADAM17-deficient ALPs yielded fewer macrophages in vitro than did wild type ALPs at the highest doses of M-CSF.
Tags: anti-mouse Ter-119 TER-119 in cancer research; anti-mouse Ter-119 TER-119 antibody in vivo

Maturation and enucleation of primitive erythroblasts during mouse embryogenesis is accompanied by changes in cell-surface antigen expression.
Fraser, S. T., et al. Blood. 2007 Jan 1;109(1):343-52. doi: 10.1182/blood-2006-03-006569. PMID: 16940424
At E9.5, nearly 90% of EryP/GFP+ cells in the peripheral blood showed low levels of Ter119 staining. ... The intensity of Ter119 fluorescence increased over the next 4 days. ... By E13.5, EryP/GFP+ cells could be roughly divided into Ter119 med and Ter119 high populations. ... From E15.5 to 17.5, essentially all EryP/GFP+ cells expressed Ter119 at high levels. ... Ter119 expression was higher in enucleated than in nucleated EryP/GFP+ cells.
Tags: anti-mouse Ter-119 TER-119 mAb in cancer research; anti-mouse Ter-119 TER-119 antibody in mouse tumor model

Erythroblasts derived in vitro from embryonic stem cells in the presence of erythropoietin do not express the TER-119 antigen.
Otani, T., et al. Exp Hematol. 2004 Jul;32(7):607-13. doi: 10.1016/j.exphem.2004.04.007. PMID: 15246156
In this serum-free culture, TER-119+ erythroblasts appeared first on day 2 and maturation proceeded until day 7. ... When EPO was added to this coculture, the number of induced floating cells increased twofold to threefold. ... Unexpectedly, the erythroid-specific antigen TER-119 expression of these cells was drastically reduced. ... Our results showed that EPO had the unusual property of inducing TER-119? erythroblasts in ES-derived primitive erythropoiesis. ... It is likely that this effect is unique to primitive erythropoiesis.
Tags: anti-mouse Ter-119 TER-119 antibody of low endotoxin; anti-mouse Ter-119 antibody TER-119

For more references about Anti-mouse Ter-119 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.