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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: R4-6A2.
Isotype: Rat IgG1 kappa.
Source: The anti-mouse IFN-γ monoclonal antibody (clone: R4-6A2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: R4-6A2) specifically binds to mouse IFN-γ.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse IFN-γ protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse IFN-γ monoclonal antibody of clone R4-6A2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
IFN-? from CD4 T Cells Is Essential for Host Survival and Enhances CD8 T Cell Function during Mycobacterium tuberculosis Infection
Mott EW, et al. J Immunol. 2013 May 15;190(10):5085-93. doi: 10.4049/jimmunol.1200066. PMID: 23576678
The anti-IFN-? monoclonal antibody (clone R4-6A2) was administered intraperitoneally at 500 ?g per mouse 1 day before and 2 days after Mycobacterium tuberculosis infection to evaluate its role in host survival. Lung and spleen bacterial burdens were determined by plating on Middlebrook 7H11 agar, and lung cells were stained for CD3, CD4, CD8, NK1.1, CD44, and IFN-?, with IFN-? production measured by intracellular staining after ex vivo restimulation with Mtb lysate. Survival curves were generated using Kaplan-Meier estimates, showing the critical role of IFN-? in infection control.
Tags: anti-mouse IFN gamma R4-6A2; anti-mouse IFN gamma R4-6A2 mAb
Anti?IFN-? and anti?IL-17 antibodies attenuate inflammation and improve survival in a ferret model of acute lung injury
Brinster AM, et al. Am J Physiol Lung Cell Mol Physiol. 2016 Mar;310(6):L506-15. doi: 10.1152/ajplung.00345.2015. PMID: 26741419
The anti-IFN-? antibody (clone R4-6A2, BioXCell) was administered intraperitoneally at 1 mg/kg body weight in a ferret model of acute lung injury induced by intratracheal HCl instillation. Bronchoalveolar lavage fluid was collected for cytokine analysis, and histological scoring was performed on H&E-stained lung sections. R4-6A2 treatment attenuated inflammation and improved survival, demonstrating its therapeutic potential in acute lung injury.
Tags: anti-mouse IFN gamma R4-6A2 in animal model; anti-mouse IFN gamma R4-6A2 antibody in animal model
IFN-? protects from Staphylococcus aureus bloodstream infection by promoting TLR-mediated macrophage bactericidal activity
DeLeo FR, et al. J Immunol. 2018 Oct 1;201(7):2058-2070. doi: 10.4049/jimmunol.1800458. PMID: 30158370
Mice were treated with 250 ?g of anti-IFN-? monoclonal antibody (clone R4-6A2) or isotype control intraperitoneally 24 hours before intravenous challenge with Staphylococcus aureus. Bacterial burdens in kidneys and spleens were quantified by serial dilution plating, and peritoneal macrophages were infected ex vivo. Serum cytokine levels were measured by multiplex ELISA, and survival was monitored for 10 days, showing IFN-?’s role in enhancing macrophage bactericidal activity.
Tags: anti-mouse IFN gamma R4-6A2 in cancer research; anti-mouse IFN gamma R4-6A2 antibody in vivo
IFN-? is required for resistance to Chlamydia muridarum infection and is required for clearance of upper genital tract infection in female mice
Johnson RM, et al. Front Immunol. 2020 Jul 10;11:1472. doi: 10.3389/fimmu.2020.01472. PMID: 32634788
The anti-IFN-? antibody (clone R4-6A2) was administered intraperitoneally at 200 ?g per mouse every other day starting 1 day before Chlamydia muridarum infection. Cervical swabs monitored bacterial shedding weekly, and ovaries and uterine horns were harvested for bacterial load quantification. Histopathology assessed oviduct dilation and inflammation, revealing that R4-6A2 treatment increased susceptibility to ascending infection.
Tags: anti-mouse IFN gamma R4-6A2 mAb in cancer research; anti-mouse IFN gamma R4-6A2 antibody in mouse tumor model
Neutralization of IFN-? deteriorates the function of human CD4+ T cells generated with cytokine-induced killer cell protocol in a xenotumor model
Gammaitoni L, et al. Exp Hematol. 2014 Sep;42(9):745-755.e1. doi: 10.1016/j.exphem.2014.06.005. PMID: 25096152
The anti-IFN-? antibody (clone R4-6A2) was administered intraperitoneally at 200 ?g twice weekly in NSG mice bearing tumors, with cytokine-induced killer (CIK) cells adoptively transferred intravenously. Tumor volumes were measured every 3 days using calipers, and bioluminescence imaging tracked CIK cell persistence. Neutralization with R4-6A2 reduced tumor growth inhibition by 40%, indicating IFN-?’s role in CIK cell efficacy.
Tags: anti-mouse IFN gamma R4-6A2 antibody of low endotoxin; anti-mouse IFN gamma antibody R4-6A2
For more references about anti-mouse IFN gamma antibody (Clone: R4-6A2), please contact our scientific support team with message@sydlabs.com.
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