Chimeric Human IgG1 Isotype Control Antibody

A chimeric human IgG1 isotype control consists of murine variable regions and human IgG1 constant regions. The chimeric human IgG1 kappa isotype control and mutants such as Fc-silenced can be used in ELISA, Western blot (WB), the cell-based assay or animal model as the antibody negative control.

A number of chimeric hIgG1 isotype control mutants are available. Most of the therapeutic antibody drugs carry the natural human IgG1 constant regions. There are some engineered mutants in the IgG1 Fc fragment. Changes to the IgG Fc sequence including glycosylation sites (such as N297A or N297G) have been used to modulate Fc functions. Even a single mutation may have both enhancement and abrogation effects on Fc binding though multiple mutation combinations are commonly used. We generated chimeric hIgG1 isotype control mutants, the same mutations as those in the therapeutic antibodies and candidates because such human IgG1 isotype control mutants are needed when clients test their engineered antibody candidates. For example, P329G substitution combined with L234A L235A (hIgG1 LALAPG, Fc silenced) was shown to decrease effector functions by disrupting the Fc/Fcγ receptor interface. The silent Fc effctor functions human IgG1 isotype controls are useful for in vivo use.

The isotype controls are normally used to differentiate any non-specific background signal from the specific antibody signal, including the immunogenicity if used in animals. Most in vivo functional grade human IgG antibodies are used in mice for in vivo studies. Besides the antibody Complementarity-Determining Regions (CDRs), two human or humanized variable regions including the one in the human or humanized IgG isotype control do not necessarily induce the same immune response in mice because in some cases, even one amino acid difference can make difference. Mouse variable regions can be used to show the potential immunogenicity induced by the human variable regions in the human or humanized IgG antibodies to test. Thus, in some cases, it is better to use the mouse variable region in the human IgG isotype controls. We also produce and sell recombinant human IgG isotype controls from the popuplar mouse hybridoma clones, MOPC21 and C1.18.4, as well as recombinant human IgG1 kappa isotype controls against KLH, TNP, DNP,  GST, HEL, His-tag, DYKDDDDK, c-myc, HA, GFP, and unknown specificity.

Please contact us to ask for a quote for the Fc silenced chimeric human IgG1 isotype control mutants, chimeric hIgG1 isotype control and silent Fc mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated chimeric hIgG1 isotype control and Fc silent mutants. A variety of conjugates (such as a fluorophore) with the chimeric human IgG1 isotype control and Fc effector-silent mutants are available.

PA007187: In Vivo Grade Recombinant Chimeric Human IgG1 kappa Isotype Control Antibody (Chimeric hIgG1 Isotype Control)

The in vivo grade recombinant chimeric human IgG1 kappa isotype control antibody (chimeric hIgG1 isotype control) was produced in CHO cells.
Immunogen: N/A.
Clone: 4F17m.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007188: In Vivo Grade Recombinant Chimeric Human IgG1-D265A kappa Isotype Control Antibody (Chimeric hIgG1 D265A Isotype Control)

The in vivo grade recombinant chimeric human IgG1-D265A kappa isotype control mutant (chimeric hIgG1 D265A isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17m.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007189: In Vivo Grade Recombinant Chimeric Human IgG1-N297A kappa Isotype Control Antibody (Chimeric hIgG1 N297A Isotype Control)

The in vivo grade recombinant chimeric human IgG1-N297A kappa isotype control mutant (chimeric hIgG1 N297A isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17m.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 N297A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007190: In Vivo Grade Recombinant Chimeric Human IgG1-L234A L235A P329G (hIgG1 LALAPG) kappa Isotype Control Antibody (Chimeric hIgG1 LALAPG Isotype Control)

The in vivo grade recombinant chimeric human IgG1-L234A L235A P329G (hIgG1 LALAPG, Fc silenced) kappa isotype control mutant (chimeric hIgG1 LALAPG isotype control) was produced in CHO cells.
Immunogen: N/A.
Clone: 4F17m.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 LALAPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007191: In Vivo Grade Recombinant Chimeric Human IgG1-D265A N297A kappa Isotype Control Antibody (Chimeric hIgG1 DANA Isotype Control)

The in vivo grade recombinant chimeric human IgG1-D265A N297A (hIgG1 DANA) kappa isotype control mutant (chimeric hIgG1 DANA isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17m.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 DANA kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007192: In Vivo Grade Recombinant Chimeric Human IgG1-S267E L328F (hIgG1 SELF) kappa Isotype Control Antibody (Chimeric hIgG1 SELF Isotype Control)

The in vivo grade recombinant chimeric human IgG1-S267E L328F (hIgG1 SELF, Fc activated) kappa isotype control mutant (chimeric hIgG1 SELF isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 4F17m.
Isotype: human IgG1, kappa.
Applications: an isotype-matched negative control for human IgG1 SELF kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

References of Chimeric Human IgG1 Isotype Control Antibody:

Monoclonal Antibody Targeting Sialyl-di-Lewisa-Containing Internalizing and Noninternalizing Glycoproteins with Cancer Immunotherapy Development Potential.
Tivadar, S. T., et al. Mol Cancer Ther. 2020 Mar;19(3):790-801. doi: 10.1158/1535-7163.MCT-19-0221. PMID: 31871270
FG129, as well as its chimeric human IgG1 variant, CH129, binds with nanomolar functional affinity to a range of colorectal, pancreatic, and gastric cancer cell lines. ...Specifically during cancer progression, altered glycosylation and sialylation of proteins and lipids have profound effects, ranging from immune evasion to increased cellular proliferation and the capacity to metastasize. ...Here we describe the generation of FG129, a murine (m) IgG1 with kappa light chain, which predominantly targets sialyl-di-Lewisa–containing glycoproteins. ...Plasma membrane lipid extract from 5 × 107 LS180 cells, incorporated into liposomes, was used to immunize BALB/c mice at two-weekly intervals over a 2-month period, with alpha-galactosylceramide and anti-CD40 mAb included as adjuvants. ...Payload delivery by FG129 was initially evaluated by measuring the cytotoxicity of immune-complexed FG129 with a saporin-conjugated anti-mouse or anti-human.
Tags: activity of Chimeric Human IgG1 Isotype Control; activity of Chimeric Human IgG1 Isotype Control mAb

Identification of Tn antigen O-GalNAc-expressing glycoproteins in human carcinomas using novel anti-Tn recombinant antibodies.
Matsumoto, Y., et al. Glycobiology. 2020 Apr;30(5):282-300. doi: 10.1093/glycob/cwz095. PMID: 31742337
More importantly, ReBaGs6 exhibited a relatively strong binding to glycoproteins with the Tn antigen (Kd = 5.43 × 10−8 M), while Remab6 as a chimeric human IgG1 showed a slightly lower binding affinity (Kd = 8.29 × 10−7 M). ...Here we generated two recombinant antibodies to the Tn antigen, Remab6 as a chimeric human IgG1 antibody and ReBaGs6 as a murine IgM antibody and characterized their specificities using multiple biochemical and biological approaches. ...Both Remab6 and ReBaGs6 recognize clustered Tn structures, but most importantly do not recognize glycoforms of human IgA1 that contain potential cross-reactive Tn antigen structures. ...In immunohistochemistry (IHC), Remab6 stains many human cancers in tissue array format but rarely stains normal tissues and then mostly intracellularly. ...The Tn antigen is a neoantigen abnormally expressed in many human carcinomas and expression correlates with metastasis and poor survival.
Tags: Chimeric Human IgG1 Isotype Control mAb; human IgG1 recombinant isotype control

Plant-based production of two chimeric monoclonal IgG antibodies directed against immunodominant epitopes of Vibrio cholerae lipopolysaccharide.
Levinson, K. J., et al. J Immunol Methods. 2015 Jul;422:111-7. doi: 10.1016/j.jim.2015.04.001. PMID: 25865265
We have produced and characterized two chimeric human IgG1 monoclonal antibodies that bind different immunodominant epitopes on Vibrio cholerae lipopolysaccharide (LPS). MAb 2D6 IgG1 recognizes Ogawa O-polysaccharide antigen, while mAb ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba LPS. ...Vibrio cholerae is the causative agent of cholera, an acute diarrheal disease that remains endemic in many parts of the world. ...In addition to seasonal outbreaks in areas where V. cholerae is endemic, natural disasters such as the 2010 earthquake in Haiti highlight the continued potential for V. cholerae to cause mass casualties in regions that were previously cholera-free. ...Serum LPS-specific IgG titers and vibriocidal activity are the two primary measures of immunity to V. cholerae. ...While these comparisons allow for relative antibody titer differences to be analyzed within a sample population, it limits comparisons across different clinical studies or vaccine trials. ...A universal human IgG antibody standard directed against one or more immunodominant epitopes on V. cholerae LPS would be of enormous benefit to the cholera research community.
Tags: Chimeric Human IgG1 Isotype Control antibody for cancer research; Chimeric Human IgG1 Isotype Control antibody for flow cytometry

For more references about Chimeric Human IgG1 Isotype Control Antibody please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following IgG Protein :
Human IgG1 Fc Protein
Human IgG1 Fc Protein
Human IgG2 Fc Protein
Human IgG3 Fc Protein
Human IgG4 Fc Protein
Llama IgG2b Fc Protein
Mouse IgG1 Fc Protein
Mouse IgG2a Fc Protein
Mouse IgG2b Fc Protein
Mouse IgG2c Fc Protein
Mouse IgG2C Protein
Mouse IgG3 Fc Protein
Rabbit IgG Protein
Rat IgG2a Fc Protein
Rat IgG2b Fc Protein

Syd Labs provides the following IgG Antibody :
Chimeric Human IgG1 Isotype Control Antibody
Chimeric Human IgG2 Isotype Control Antibody
Chimeric Human IgG4-S228P Isotype Control Antibody
Hamster IgG1 Isotype Control Antibody
Hamster IgG2 Isotype Control Antibody
Human IgG1 Isotype Control Antibody
Human IgG2 Isotype Control Antibody
Human IgG3 Isotype Control Antibody
Human IgG4-S228P Isotype Control Antibody
IgG Antibody
Mouse IgG1 Isotype Control Antibody
Mouse IgG2a Isotype Control Antibody
Mouse IgG2b Isotype Control Antibody
Mouse IgG2c Isotype Control Antibody
Mouse IgG3 Isotype Control Antibody
Rat IgG1 Isotype Control Antibody
Rat IgG2a Isotype Control Antibody
Rat IgG2b Isotype Control Antibody