Name: Anti-Mouse CD3e Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007521.r2a
Price: 150.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007521.r2a

In Vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: KT3), Rat IgG2a Kappa

1 mg

150.00

PA007521.r2a

In Vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: KT3), Rat IgG2a Kappa

5 mg

350.00

PA007521.r2a

In Vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: KT3), Rat IgG2a Kappa

25 mg

900.00

Description

PA007521.r2a: In Vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: KT3), Rat IgG2a Kappa

Recombinant Rat IgG2a Monoclonal Antibody.
Clone: KT3.
Isotype: Rat IgG2a kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: KT3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: KT3) specifically binds to the mouse T cell receptor CD3e.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3e protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse CD3e Monoclonal Antibody:

Lung-resident CD4⁺ T cells are sufficient for IL-4Rα-dependent recall immunity to Nippostrongylus brasiliensis infection.
Thawer, S. G., et al. Mucosal Immunol. 2014 Mar;7(2):239-48. doi: 10.1038/mi.2013.40. PMID: 23778354
Rat anti-mouse CD3 (KT3) was purchased from Abcam. ...Data were expressed as mean±s.d. and analyzed using one-tailed Mann–Whitney nonparametric T test with a 95% confidence interval. ...The work presented was funded by National Research Foundation, Medical Research Council (South Africa), International Centre for Genetic Engineering and Biotechnology, Worldwide Universities Network, and Claude Leon Foundation. ...Whole lung was removed from individual mice, finely cut, and digested in Iscove's modified Eagle medium (Invitrogen, Carlsbad, CA) containing 50 U ml−1 collagenase type I (Invitrogen) and 13 μg ml−1 DNase (Roche, Carlsbad, CA) at 37 °C for 90 min. ...Lung worm burdens were quantified as previously described8 by finely cutting the lung, placing on sterile gauze, and suspending them in a 50-ml centrifuge tube containing phosphate-buffered saline at 37 °C for at least 3 h.
Tags: anti-mouse CD3e KT3 antibody; anti-mouse CD3e KT3 antibody in vivo

Lymphoid tissue and plasmacytoid dendritic cells and macrophages do not share a common macrophage-dendritic cell-restricted progenitor.
Sathe, P., et al. Immunity. 2014 Jul 17;41(1):104-15. doi: 10.1016/j.immuni.2014.05.020. PMID: 25035955
The mAb hybridoma clones used were CD2 (RM2-1), CD3 (KT3), CD8 (YTS169.4 or 53-6.7), CD19 (1D3) CD45R (RA36B2), CD11b (M1/70), TER119 (TER119), Ly6G (1A8), CD45.1 (A20.1), CD45.2 (S450-15.2), CD11c (N418), F4/80 (F4/80), CD34 (RAM34), sca-1 (E13 161-7), c-kit (ACK2), flt3 (A2F10.1), CD16/32 (2.4G2), and M-CSFR (AFS98). ...Mice were 6–12 weeks of age. Unless otherwise indicated, experiments were performed using C57BL/6J Wehi (CD45.2) mice. ...Mice were bred under specific-pathogen-free conditions at the Walter and Eliza Hall Institute; breeding and procedures were approved by the Animal Ethics Committee, WEHI. For analysis of precursors in FL-deficient mice, BM was extracted from C57BL/6-flt3Ltm1Imx (FL−/−) mice from the University of Western Australia Animal Care Facility under the guidelines of the UWA Animal Ethics Office. ...Erythrocytes, dead cells, and very dense cells, were eliminated from BM cell suspensions by centrifugation in Nycodenz (Nycomed Pharma) medium (1.086 g/cm3, 4°C, mouse osmolarity). ...Progenitors were isolated from CX3CR1-GFP or CD45.1 BM and 5 × 104–2 × 105 cells transferred intravenously into lethally irradiated CD45.2 recipients.
Tags: anti-mouse CD3e KT3 in animal model; anti-mouse CD3e KT3 mAb in animal model

For more references about Anti-mouse CD3e Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.