Anti-Mouse CD3 Monoclonal Antibodies for Flow Cytometry

PA007254.r2b: Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b kappa

Recombinant  rat IgG2b monoclonal antibody. 
Clone: 17A2.
Isotype:  Rat IgG2b kappa.
Source: The recombinant anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant rat monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007254.m2a: Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2a kappa

Recombinant  mouse IgG2a monoclonal antibody. 
Clone: 17A2.
Isotype: Mouse IgG2a kappa.
Source: The recombinant anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 17A2 antibody specifically binds to the mouse T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007255.m2a-b: Biotinylated Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2a kappa

Recombinant mouse IgG2a monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 17A2.
Isotype: Mouse IgG2a kappa.
Source: The site-directed biotinylated anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant mouse monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The 17A2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse CD3 antibody (17A2)

Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Sugita S, Shimizu J, Makabe K, Keino H, et al. (2017). Arthritis Res Ther. 2017 Jul 25;19(1):176. doi: 10.1186/s13075-017-1379-9. PMID: 28743289
In brief, CFSE-labeled C57BL/6JJcl splenocytes (2 × 10^6 cells/well) and irradiated (20 Gy) BALB/c splenocytes (2 × 10^5 cells/well) were cocultured in 24-well plates with 1 μg/ml of Dow2, a control anti-mouse CD3 ε Ab (clone 17A2), or an isotype control (rat IgG)...Mouse pan-T cells isolated from C57BL/6JJcl donors were incubated with 1 μg/ml of Dow2 or a control anti-mouse CD3 Ab (clone 17A2 or clone 145-2C11) at 4 °C for 30 min...Incubation with Dow2 alone or rat IgG did not stimulate T cells to produce detectable IFN-γ (Fig. 2b, left panel) or IL-17 (Fig. 2c, left panel). In contrast, T cells incubated with 17A2 (anti-mouse CD3 antibody) produced increased levels of cytokines which were significantly reduced in T cells incubated with both Dow2 and 17A2 (anti-mouse CD3 mAb)...In contrast, IRBP-stimulated production of IFN-γ was significantly enhanced in ocular cells incubated with 17A2 antibody (P < 0.005)...The expression of CD4 and CD8 on splenocytes from normal control mice and Dow2- or rat IgG-treated EAU mice was assessed by flow cytometry.
Tags: anti-mouse CD3; 17A2 antibody

In vivo Imaging Technologies to Monitor the Immune System
McCarthy CE, White JM, Viola NT, Gibson HM. (2020). Front Immunol. 2020 Jun 2;11:1067. doi: 10.3389/fimmu.2020.01067. PMID: 32582173
Upon ex vivo analysis, one study reported a relative depletion of CD4 T cells and an expansion of CD8 T cells after delivery of an 89Zr-anti-CD3 tracer (clone 17A2) in mice...Conjugation of the contrast material to an antibody allows for specific targeting of a cell population or protein of interest...Protein platforms including antibodies, cytokines, and receptor ligands are also popular choices as molecular imaging agents for positron emission tomography (PET), single-photon emission computerized tomography (SPECT), scintigraphy, and optical imaging...Promising new adoptive cell therapies (ACT) for cancer, including ex vivo expanded tumor-infiltrating lymphocytes (TILs) and chimeric antigen receptor T cells (CAR-T cells), would benefit from imaging technologies that track cell fate in vivo...CD8 + cytotoxic T lymphocytes (CTLs) specific to the melanoma antigen Melan-A were 111In-labeled, infused in patients, and evaluated using serial whole body and static gamma camera imaging.
Tags: anti-mouse CD3 [Clone 17A2] - purified; 17A2 mAb for flow cytometry

Quantitative CD3 PET Imaging Predicts Tumor Growth Response to Anti-CTLA-4 Therapy
Larimer BM, Wehrenberg-Klee E, Caraballo A, Mahmood U. (2016). J Nucl Med. 2016 Oct;57(10):1607-1611. doi: 10.2967/jnumed.116.173930. PMID: 27230929
The monoclonal antimouse CD3 antibody clone 17A2 was purchased from...Conjugation and radiolabeling of the CD3 antibody resulted in a pure, highly specific probe for CD3-expressing cells...For Western blotting, the tumors were lysed in 1% sodium dodecyl sulfate and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis...Blots were transferred to nitrocellulose, blocked with 5% nonfat dry milk, and probed with the appropriate antibodies...Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 10-μm slices of tumor to confirm the cellular location of CD3 expression.
Tags: anti-mouse CD3 antibody (clone 17A2); anti-mouse CD3 antibody for flow cytometry

For more references about anti-mouse CD3 antibody (17A2), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
Biotin Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
FITC Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
APC Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
PE Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
Pacific Blue Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
PerCP Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
PE/Cy5 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
PE/Cy7 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
iFluor 488 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
iFluor 549 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
iFluor 594 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
iFluor 647 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
iFluor 700 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
APC/Cy7 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)
PerCP/Cy5.5 Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)

Background:

The 17A2 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes.

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry