Anti-Human CD3e Monoclonal Antibodies for Flow Cytometry

PA007417.m2a: Non-conjugated Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa

Recombinant  Mouse IgG2a Monoclonal Antibody. 
Clone: 12F6.
Isotype:  Mouse IgG2a kappa.
Source: The anti-human CD3e monoclonal antibody (clone: 12F6) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 12F6 antibody specifically binds to human CD3e.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007417.h1Fs: Non-conjugated Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Human IgG1 Fc Silent Kappa

Recombinant  Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 12F6.
Isotype: Human IgG1 kappa.
Source: The anti-human CD3e monoclonal antibody (clone: 12F6) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 12F6 antibody specifically binds to human CD3e.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007753.m2a-b: Biotinylated Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa

Recombinant  Mouse IgG2a Monoclonal Antibody with site-directed  biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 12F6.
Isotype:  Mouse IgG2a kappa.
Source: The site-directed biotinylated anti-human CD3e monoclonal antibody (clone: 12F6) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 12F6 antibody specifically binds to human CD3e.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 12F6 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background:

The 12F6 antibody competes with the OKT3 antibody for binding to human T cells, and has more effective T-cell suppression and activation properties than OKT3. The 12F6 antibody is used to study T-cell functions and to manipulate T-cell activation and suppression, prevent acute allograft rejection, and treat autoimmune diabetes.

References of anti-human CD3e antibody (12F6)

Polymorphic residues in HLA-B that mediate HIV control are associated with enhanced structural rigidity
Ramarathinam, S., et al. (2024). Structure. 32(8):1088-1100.e6. doi: 10.1016/j.str.2024.04.015. PMID: 38795288
T2 cells pulsed with 100 μM peptide were stained with anti-human CD3 (clone 12F6) and analyzed by flow cytometry to confirm T cell activation. Peptide binding to HLA-B was quantified using a fluorescence-based assay to assess structural rigidity. The study identified specific HLA-B residues that enhance peptide-MHC stability in HIV controllers. Flow cytometry data showed increased CD3 expression in T cells from HIV controllers compared to progressors. Structural analysis revealed that polymorphic residues contribute to a rigid HLA-B conformation, impacting T cell responses.
Tags: activity of 12F6 antibody; function of 12F6 antibody

Simultaneous assessment of cytotoxic T lymphocyte responses in individuals with chronic hepatitis B virus infection
Alatrakchi, N., et al. (2022). J Viral Hepat. 29(5):347-357. doi: 10.1111/jvh.13654. PMID: 35133674
PBMCs were stained with anti-CD3 (clone 12F6) antibody and anti-CD8 (clone RPA-T8) for flow cytometric analysis of CTL responses. HBV-specific CTL responses were measured using peptide-MHC tetramers in chronic HBV patients. The frequency of CD8+ T cells producing IFN-γ was significantly higher in patients with controlled HBV infection. Flow cytometry revealed distinct T cell subsets in responders versus non-responders, highlighting the role of cytotoxic T lymphocytes in HBV immune control.
Tags: 12F6 antibody for animal model; 12F6 antibody for cancer research

Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving
Varadarajan, N., et al. (2012). Proc Natl Acad Sci U S A. 109(10):3885-90. doi: 10.1073/pnas.1111200109. PMID: 22307670
After microengraving, cells were stained with fluorescently labeled antibodies against CD3 (clone 12F6, anti-human CD3e antibody) and CD8 to identify HIV-specific T cells. Microengraving allowed rapid identification and recovery of antigen-specific T cells. HIV-specific CD8+ T cells were sorted based on cytokine secretion profiles. The technique enabled high-throughput analysis of T cell functionality, with flow cytometry confirming the specificity of recovered T cells to HIV epitopes.
Tags: 12F6 antibody for flow cytometry; 12F6 antibody for mouse tumor model

Phase I trial of a humanized, Fc receptor nonbinding anti-CD3 antibody, visilizumab (Nuvion) in treatment-resistant severe ulcerative colitis
Sandborn, W. J., et al. (2010). MAbs. 2(4):444-55. doi: 10.4161/mabs.2.4.12305. PMID: 20564327
Flow cytometry analysis used anti-CD3 (clone 12F6) antibody to monitor T cell depletion in peripheral blood samples from ulcerative colitis patients. Visilizumab treatment resulted in significant reduction of circulating T cells within 24 hours. The study evaluated the safety and efficacy of visilizumab in severe ulcerative colitis, noting cytokine release syndrome in a subset of patients. T cell recovery was monitored over 28 days using flow cytometry.
Tags: 12F6 mAb for mouse tumor model; 12F6 monoclonal antibody

Profiles of cytokines produced by CD4-positive T lymphocytes in response to anti-CD3 antibody
Hisada, T., et al. (1998). J Gastroenterol. 33(4):505-11. doi: 10.1007/s005350050122. PMID: 9741358
The anti-CD3 monoclonal antibody (12F6) was used to stimulate CD4+ T cells, followed by cytokine detection via flow cytometry. IL-2 and IFN-γ production were significantly elevated in CD4+ T cells post-stimulation. The study analyzed cytokine profiles in healthy versus inflammatory bowel disease patients, revealing distinct Th1 cytokine patterns in response to anti-CD3 stimulation through intracellular cytokine staining.
Tags: 12F6 mAb; 12F6 antibody mouse dose range

For more references about anti-human CD3e antibody (12F6), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)