Name: Anti-Mouse CD3e Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007198
Price: 150.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007198

In vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

1 mg

150.00

PA007198

In vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

5 mg

350.00

PA007198

In vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

25 mg

900.00

Description

PA007198: In vivo Grade Recombinant Anti-mouse CD3e Mouse IgG2a Kappa Monoclonal Antibody (Clone: 145-2C11)

Recombinant mouse IgG2a Monoclonal Antibody.
Clone: 145-2C11.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: 145-2C11) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 145-2C11) specifically binds to the mouse T cell receptor CD3e.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3e protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-mouse CD3e Monoclonal Antibody:

Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells.
Kim YU et al. PLoS One. 2015 Mar 13;10(3):e0120294. doi: 10.1371/journal.pone.0120294. PMID: 25768299
After washing, slides were incubated with BlockAid Blocking Solution (Life Technologies) for 30 min, and then stained with Biotinylated Peanut Agglutinin (PNA, Vector Laboratories, Burlingame, CA), and hamster anti-mouse CD3e (clone 145?2C11, BioXcell, West Lebanon, NH). The slides were further incubated with Pacific Blue-conjugated anti-IgD (clone, 11?26c.2a, BioLegend), DyLight649-conjugated anti-Hamster IgG (clone Poly4055, BioLegend), and DyLight594-conjugated Streptavidin (Pierce Biotechnology, Rockford, IL). ...For in vitro antibody production assay, sorted 1 × 105 Na?ve B cells and diverse ratio of CXCR5+ or CCR6+ CD4 T cells or in vitro differentiated Th17 cells were co-cultured in the presence of 2 ?g/ml soluble anti-CD3e (clone 145?2C11, BioXcell) and anti-IgM (AffiniPure F(ab’)2 Fragment Goat anti-IgM, ? chain specific, Jackson ImmunoResearch) for 7 days. ...To measure the levels of antibodies to dsDNA and histone in circulation, serum was collected from the wild type or BXD2 mice at various time points and was measured by ELISA as described previously. ...Total RNA was extracted from splenocytes (1×106 cells) or in vitro co-cultured cells with TRIzol (Invitrogen) and reverse transcribed using amfiRivert reverse transcriptase (GenDepot, Baker, TX) according to the manufacturer’s protocol. ...As a first step to determine the contribution of each T helper (Th) cell response to autoimmune lupus in BXD2 mice, we comparatively analyzed the production of autoantibodies and the generation of spontaneous germinal center reactions between BXD2 mice and C57BL/6 (wild-type) control mice at the age of 6 months or older.
Tags: anti-mouse CD3e 145-2C11; anti-mouse CD3e 145-2C11 antibody

TGF-? signalling is required for CD4? T cell homeostasis but dispensable for regulatory T cell function.
Sledzi?ska A et al. PLoS Biol. 2013 Oct;11(10):e1001674. doi: 10.1371/journal.pbio.1001674. PMID: 24115907
The cells were cultured for 40 min in the presence of antiCD3/CD28 either with or without 20 ng/ml TGF?-1. ...These are representative data of two independent experiments. ...Two weeks p.a. the surface expression of TR2 was reduced only slightly on CD4+ thymocytes but not on any of the other major thymocyte compartments. ...Because mild autoimmunity may not present clinically, we performed histopathological analysis of liver, kidney, pancreas, heart, colon, and thyroid gland but could not detect cellular infiltrates in any of these organs (unpublished data). ...Abrogation of TGF-? signalling through CD4-Cre takes place during thymic development and affects all ??T cells including Treg cells.
Tags: anti-mouse CD3e 145-2C11 in vivo; anti-mouse CD3e 145-2C11 mAb in vivo

Anti-CD3 antibodies modulate anti-factor VIII immune responses in hemophilia A mice after factor VIII plasmid-mediated gene therapy.
Peng et al. Blood. 2009 Nov 12;114(20):4373-82. doi: 10.1182/blood-2009-05-217315. PMID: 19770362
Anti?mouse-CD3? monoclonal antibodies (145-2C11), anti?mouse-CD25 monoclonal antibodies (PC61), and mouse IgG1 isotype control were purchased from BioXCell. ...Therapeutic biologic compounds have been used successfully to modulate the immune system. The development of the first humanized anti-CD3 monoclonal antibody (OKT3) spurred a spate of studies with CD3-specific antibodies for the treatment of immune-mediated diseases. ...Anti-CD3 therapy was demonstrated to promote, in both transplantation21,22 and autoimmune settings,23 antigen-specific immune tolerance. ...It was suggested that the combination therapy induces large numbers of islet cell-specific regulatory T cells (Tregs), whereas anti-CD3 or proinsulin alone was insufficient to prime Tregs. ...Here we report the successful use of the anti-CD3 monoclonal antibody in modulating immune responses against FVIII after gene therapy.
Tags: anti-mouse CD3e 145-2C11 antibody in animal model; anti-mouse CD3e 145-2C11 mAb in animal model

For more references about Anti-mouse CD3e Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.