Name: Anti-Mouse CD3e Monoclonal Antibodies
Brand: Syd Labs
SKU: PA007198
Price: 150.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

PA007198

In vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

1 mg

150.00

PA007198

In vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

5 mg

350.00

PA007198

In vivo Grade Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

25 mg

900.00

Description

PA007198: In vivo Grade Recombinant Anti-mouse CD3e Mouse IgG2a Kappa Monoclonal Antibody (Clone: 145-2C11)

Recombinant mouse IgG2a Monoclonal Antibody.
Clone: 145-2C11.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: 145-2C11) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 145-2C11) specifically binds to the mouse T cell receptor CD3e.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3e protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse CD3e antibody (145-2C11)

Potent antitumor activity of a designed interleukin-21 mimic
Sockolosky JT, et al. (2024). bioRxiv [Preprint]. 2024 Dec 7:2024.12.06.626481. doi: 10.1101/2024.12.06.626481. PMID: 39677740
Purified murine CD4+ and CD8+ T cells were pre-activated with plate-bound anti-mouse CD3 (2 ?g/mL; clone 145-2C11) and soluble anti-mouse CD28 (1 ?g/mL; clone 37.51) and cultured in complete RPMI media for 48 hours at 37°C...Purified murine CD8+ T cells were pre-activated with plate-bound anti-mouse CD3 (2 ?g/mL; clone 145-2C11) and soluble anti-mouse CD28 (1 ?g/mL; clone 37.51) and cultured in complete RPMI media for 48 hours at 37°C...One day prior to activation, tissue culture plates were coated with 1 ?g/mL anti-CD3? (BioLegend, Ultra-LEAF, Clone 145-2C11) and 0.5 ?g/mL anti-CD28 (CyTek in vivo ready, Clone 37.51) in PBS and incubated overnight at 4°C...In vitro, tumor-targeting CAR-T cells generated in the presence of 21h10 exhibited enhanced cell expansion and cytotoxicity, with increased expression of CD25, granzyme B, and Bcl2...21h10 treatment induced immune memory in MC38 treated mice, as rechallenged animals were protected.
Tags: anti-mouse CD3e 145-2C11; anti-mouse CD3e 145-2C11 in vivo

Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Oca?a-Guzmán R, et al. (2022). Cells. 2022 May 13;11(10):1635. doi: 10.3390/cells11101635. PMID: 35626672
For this evaluation, cells were collected and stained for 20 min at 4 °C with mAb to CD3, TNF, CD11b, and F4/80...To obtain 1.2 × 10^7 RAW CD3+ cells, we usually sorted 4 × 10^7 total RAW cells...After labeling, cells were resuspended in 5 mL of PBS plus 1% FBS to be acquired and sorted in the FACS-Aria-II cytometer...Indicated wells were coated with the anti-CD3 antibody or isotype control at 1 ?g/mL in sterile PBS and incubated at 5% CO2 at 37 °C overnight a day before the experiment...IP was performed using Dynabeads Protein G Kit and monoclonal antibodies for mouse CD3.
Tags: anti-mouse CD3e 145-2C11 in animal model; function of anti-mouse CD3e 145-2C11

In vivo induction of interleukin 10 by anti-CD3 monoclonal antibody therapy
Zheng XX, et al. (1993). J Clin Invest. 1993 Feb;91(2):547-53. doi: 10.1172/JCI116234. PMID: 8426125
We investigated the in vivo effects of cyclosporin A (CsA) on the production of interleukin (IL) 10, a cytokine with major immunosuppressive properties...Anti-CD3 monoclonal antibody was administered intraperitoneally to mice...The antibody used was clone 145-2C11...In vivo treatment induced IL-10 production...CsA modulated the cytokine response in treated animals.
Tags: anti-mouse CD3e 145-2C11; 145-2C11 antibody

Effects of in vivo administration of anti-CD3 monoclonal antibody on T cell function in mice. II. In vivo and in vitro immunosuppression
Hirsch R, et al. (1989). J Immunol. 1989 Feb 1;142(3):737-43. PMID: 2521507
The present study demonstrates that T cell activation does occur after in vivo administration of anti-CD3 mAb to mice, evidenced by increased IL-2R expression...The anti-CD3 mAb used was 145-2C11...In vivo administration led to immunosuppression...In vitro assays showed reduced T cell function...The study involved multiple doses in mice models.
Tags: anti-mouse CD3e 145-2C11 mAb; 145-2C11 mAb

For more references about anti-mouse CD3e antibody (145-2C11), please contact our scientific support team with message@sydlabs.com.