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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-human CD3 Monoclonal Antibodies for Flow Cytometry

Anti-human CD3 Monoclonal Antibodies for Flow Cytometry

cluster of differentiation 3, CD3D, CD3E, CD3G

Catalog No. Product Name Size List Price (US$) Quantity
PA007222.m1 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1), Mouse IgG1 Kappa 50 ug 45.00
PA007222.m1 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1), Mouse IgG1 Kappa 200 ug 90.00
PA007222.h1Fs Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1), Human IgG1 Fc Silent Kappa 50 ug 45.00
PA007222.h1Fs Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1), Human IgG1 Fc Silent Kappa 200 ug 90.00
Description

PA007222: Non-conjugated Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)

Recombinant mouse IgG1 Monoclonal Antibody.
Clone: UCHT1.
Isotype: Mouse IgG1 kappa.
Source: The anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: UCHT1) specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
 

PA007223: Biotin Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)

Recombinant mouse IgG1 Monoclonal Antibody with site-directed biotin conjugation through the Avi tag.
Clone: UCHT1.
Isotype: Mouse IgG1 kappa.
Source: The biotin anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Conjugation: biotin-conjugated.
Specificity/Sensitivity: The biotin recombinant mouse monoclonal antibody (clone: UCHT1) specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-human CD3e antibody (UCHT1):


Monovalent Anti-CD3 Antibodies Effectively Eliminate the TCR-Positive Fraction of TCR-Deleted Allogeneic CAR-T Cells to Prevent GVHD
Kim JH, et al. Immune Netw. 2024 Dec;24(6):e43. doi: 10.4110/in.2024.24.e43. PMID: 39801735
This study investigates the use of monovalent anti-CD3 antibodies, OKT3 and UCHT1, to eliminate TCR-positive allogeneic CAR-T cells to prevent graft-versus-host disease (GVHD). Full-length OKT3-IgG and UCHT1-IgG were purchased from Bio X Cell and BioLegend, respectively. Fab fragments were generated by cloning VH-CH1 and VL-CL domains into pCEP4 plasmids and transfecting FreeStyle? 293-F cells. Single-chain variable fragments (scFvs) for OKT3, UCHT1, and other clones were cloned into a pCEP4 vector with a human kappa chain constant region. TCR deletion was assessed by flow cytometry using anti-TCR??-PE/Cy5 (IP26) and anti-CD3?-PE (UCHT1) antibodies. Unlike full-length UCHT1-IgG, UCHT1-scFv induced significant apoptosis in expanded T cells.
Tags: activity of UCHT1 antibody; function of UCHT1 antibody

Comprehensive analysis of human monocyte subsets using full-spectrum flow cytometry and hierarchical marker clustering
Li C, et al. Front Immunol. 2024 May 20;15:1405249. doi: 10.3389/fimmu.2024.1405249. PMID: 38742110
This study employs full-spectrum flow cytometry to analyze human monocyte subsets, utilizing a panel of monoclonal antibodies including PerCP-conjugated CD45, BV510-conjugated CD3, and others detailed in the study. T cells were isolated using a CD3/CD14 plot, with CD3+ T cells further divided into ??/?? T cells via a CD3/TCR-?? plot. NKT cells were distinguished from ?? T cells using a CD56/CD3 plot. CD19+ B cells were defined within the CD3+ T cell-excluded PBMC compartment. The study provides a comprehensive analysis of immune cell populations using hierarchical marker clustering.
Tags: UCHT1 antibody; UCHT1 antibody for cancer research

A new flow cytometry assay identifies recipient IgG subtype antibodies binding donor cells: increasing donor availability for highly sensitised patients
Rao PN, et al. Clin Transl Immunol. 2022 Sep 19;11(9):e1415. doi: 10.1002/cti2.1415. PMID: 36092480
This study describes a flow cytometry assay to detect recipient IgG subtype antibodies binding to donor cells, improving donor availability for highly sensitized patients. The assay uses a lyophilized reagent containing PE/Cy7-conjugated anti-human CD3 antibody (clone UCHT1) for T cells and APC/Fire? 750-conjugated anti-human CD19 antibody (clone HIB19) for B cells. Cells were stained with a six-color lyophilized antibody reagent and analyzed on a BD FACSLyric? system using BD FACSuite? RUO v1.4 software. A separate compensation control for T cells was prepared with PE/Cy7-conjugated anti-CD3 antibody.
Tags: UCHT1 antibody for flow cytometry; UCHT1 antibody for mouse tumor model

A flow cytometry-based proliferation assay for clinical evaluation of T-cell memory against SARS-CoV-2
Lind Enoksson S, et al. J Immunol Methods. 2021 Dec;499:113159. doi: 10.1016/j.jim.2021.113159. PMID: 34597619
This study presents a flow cytometry-based proliferation assay (FASCIA) to evaluate T-cell memory against SARS-CoV-2. CD3+ T-lymphocytes were analyzed using an Aquios CL flow cytometer with a monoclonal antibody mixture including anti-CD3-PC5 (clone UCHT1). Samples were stained with CD3-FITC/CD4-PE Simultest, and blasts were identified as high FSC cells, divided into CD4 (CD3+CD4+) and CD8 (CD3+CD4-) populations using KaluzaC software. SARS-CoV-2 nucleocapsid and spike proteins were tested, with pokeweed mitogen as a positive control for T cell blast formation.
Tags: UCHT1 antibody mouse dose range; UCHT1 mAb for animal model

B7-H7 (HHLA2) inhibits T-cell activation and proliferation in the presence of TCR and CD28 signaling
Rieder SA, et al. Cell Mol Immunol. 2021 Jun;18(6):1503-1511. doi: 10.1038/s41423-020-0361-7. PMID: 32005952
This study examines the inhibitory effects of B7-H7 (HHLA2) on T-cell activation and proliferation in the presence of TCR and CD28 signaling. The anti-CD3 antibody clone UCHT1 was used alongside OKT3 to evaluate T-cell activation. B7-H7, combined with UCHT1, induced expression of CD25, CD69, and PD-1 on T cells, with less pronounced coinhibitory effects compared to OKT3. Plate coating included anti-CD3 UCHT1, anti-CD3 OKT3, IgG control, B7.1 Fc chimera, and B7-H7 Fc chimera, demonstrating differential impacts on T-cell activation.
Tags: UCHT1 mAb for flow cytometry; UCHT1 monoclonal antibody

For more references about anti-human CD3e antibody (UCHT1), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Biotin Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
FITC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
APC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Pacific Blue Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PerCP Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE/Cy5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 488 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 549 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 594 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 647 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 700 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
APC/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PerCP/Cy5.5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)

Background

The UCHT1 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, UCHT1, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the UCHT1 antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the UCHT1 antibody, T cells disappear from the circulation within minutes to hours. During treatment with the UCHT1 antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the UCHT1 antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the UCHT1 antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the UCHT1 antibody in vivo. The UCHT1 antibody is ineffective clinically if the CD3 target molecule is not modulated.

The UCHT1 antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry

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