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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The mouse anti-human CD3 monoclonal antibody (clone No. UCHT1, available in classic mouse IgG1 kappa and engineered formats) reacts specifically with the human CD3ε (epsilon) chain encoded by the human CD3E gene, a crucial transmembrane subunit of the T-cell receptor (TCR)-CD3 complex. Expressed uniformly on all mature lineage T cells and medullary thymocytes, the CD3 epsilon chain serves as an indispensable anchor for adaptive cellular immunity mapping. In multi-color immunophenotyping configurations, clone UCHT1 is recognized as an industry-standard lineage marker to accurately segregate T-helper (CD4+) and cytotoxic T (CD8+) populations, making it a foundational tool for anti-human cd3e monoclonal antibody flow cytometry gating cascades.
Our recombinant anti-human cd3 ucht1 flow cytometry portfolio is expressed via advanced mammalian platforms to guarantee high structural purity and exceptional lot-to-lot consistency. To resolve the common laboratory challenge of non-specific binding on Fc-receptor-rich cells (such as monocytes or macrophages), our catalog features an innovative fc silent anti-human cd3 antibody matrix (Human IgG1 Fc Silent Kappa platform). Available in diverse formulations including non-conjugated pure versions and high-affinity biotinylated recombinant anti-human cd3 ucht1 formats, these reagents enable maximum staining resolution and seamless integration into multiplex single-cell immunophenotyping panels without generating background artifacts.
Recombinant mouse IgG1 Monoclonal Antibody.
Clone: UCHT1.
Isotype: Mouse IgG1 kappa.
Source: The recombinant anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: UCHT1) specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: UCHT1.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The recombinant anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant UCHT1 antibody specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: UCHT1.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Conjugation: biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant UCHT1 antibody specifically binds to the human T cell receptor CD3.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
A classic bottleneck in immune cell gating is the non-specific binding of antibody Fc regions to cellular Fcγ receptors (FcγRs) on monocytes, macrophages, and NK cells, which triggers false-positive fluorescence signals. Our innovative fc silent anti-human cd3 antibody (such as catalog series PA007222.h1Fs) contains engineered mutations within the human IgG1 constant domain that eliminate binding affinity to FcγRs. This design allows investigators to skip expensive, time-consuming Fc-block pre-treatment steps while executing ultra-clean recombinant anti-human cd3 ucht1 flow cytometry gating cascades.
Our biotinylated recombinant anti-human cd3 ucht1 configuration (PA007233.h1Fs-b) is specifically optimized for high-sensitivity multi-color flow cytometry and indirect signal amplification. By leveraging a secondary Streptavidin-fluorophore conjugate step, it provides an outstanding signal-to-noise ratio for detecting low-abundance T-cell sub-compartments. Furthermore, this format provides excellent multi-color panel design flexibility, allowing you to easily shift your CD3 fluorophore channels independent of standard direct-conjugate restrictions without introducing Fc-binding artifacts.
Syd Labs offers structured catalog diversity to match distinct assay controls perfectly. Our standard recombinant platform delivers the classic anti-human cd3 clone ucht1 mouse igg1 (kappa) format (PA007222.m1), which targets the anti-human cd3e monoclonal antibody flow cytometry epsilon chain subunit. If your experimental pipeline requires an all-human constant framework, we provide the engineered Human IgG1 Fc Silent Kappa platform. This defined recombinant nature guarantees 100% sequence uniformity across lots, ensuring that your isotype control baselines remain flawlessly consistent from one screening sprint to the next.
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Biotin Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
FITC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
APC Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Pacific Blue Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PerCP Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE/Cy5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PE/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 488 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 549 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 594 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 647 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
iFluor 700 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
APC/Cy7 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
PerCP/Cy5.5 Recombinant Anti-human CD3e Monoclonal Antibody (Clone: UCHT1)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry
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