Anti-Mouse CD3e Monoclonal Antibodies for Flow Cytometry

PA007492.m2a: Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 500A2), Mouse IgG2a Kappa

Recombinant Mouse IgG2a Monoclonal Antibody. 
Clone: 500A2.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: 500A2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 500A2 antibody specifically binds to mouse T cell receptor CD3e.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007492.h1Fs: Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 500A2), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 500A2.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: 500A2) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 500A2 antibody specifically binds to mouse T cell receptor CD3e.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 500A2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

References of anti-mouse CD3e antibody (500A2)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza A, Chang HD, Radbruch A, et al. (2021). Eur J Immunol. 51(12):2708-3145. doi: 10.1002/eji.202149124. PMID: 34910301
The anti-CD3 antibody clone 500A2 is recommended for mouse T cell identification in multicolor flow cytometry panels. For surface staining, antibodies were used at predetermined optimal dilutions in FACS buffer for 20 min at 4°C in the dark. Cells were washed twice with FACS buffer and fixed in 1% paraformaldehyde. Dead cells were excluded using a viability dye such as Zombie Aqua. Flow cytometry data were acquired on a BD FACSCanto II and analyzed using FlowJo software.
Tags: 500A2 antibody; anti-mouse CD3e 500A2 for flow cytometry

Combining ibrutinib and checkpoint blockade improves CD8+ T-cell infiltration and antitumor efficacy in the mesothelioma model
Uslu U, Schuler PJ, Winter C, et al. (2023). Haematologica. 108(3):825-835. doi: 10.3324/haematol.2022.281172. PMID: 36073525
anti-Mouse CD3e 500A2 was used for flow cytometric identification of CD8+ T cells in tumor infiltrates. Cells were incubated with recommended dilutions of antibodies against cell surface proteins in PBS containing 0.1% fixable viability dye for 30 minutes at 4°C. For labelling of cells in whole blood, 50-100 μl of PB were stained with antibodies specific for surface molecules for 30 minutes at 4°C, followed by incubation for 10 minutes with 2 ml of 1X BD FACS lysing solution to remove erythrocytes. Cytotoxic function of CD8+ effector T-cells was assessed by flow cytometric analysis of degranulation capacity, as measured by CD107a expression on the cell surface, GzmA and GzmB expression or IFNγ production. Flow cytometry data were analyzed using FlowJo software version 10.
Tags: function of anti-mouse CD3e 500A2; anti-mouse CD3e [Clone 500A2] - purified

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Cossarizza A, Chang HD, Radbruch A, et al. (2019). Eur J Immunol. 49(10):1457-1973. doi: 10.1002/eji.201970117. PMID: 29915044
The clone 500A2 anti-CD3 antibody is suggested for gating T cells in mouse flow cytometry experiments. Antibody staining was performed in PBS with 2% FBS and 0.09% sodium azide for 30 min on ice. Samples were acquired on a BD LSRFortessa and data analyzed with FlowJo v10. Compensation was performed using single-stained controls. Viability was assessed with propidium iodide.
Tags: anti-CD3e (500A2) monoclonal antibody; clone 500A2 of anti-mouse CD3e

Guidelines for the use of flow cytometry and cell sorting in immunological studies
Cossarizza A, Boros P, Antognoli G, et al. (2017). Eur J Immunol. 47(10):1584-1797. doi: 10.1002/eji.201747399. PMID: 26364554
Anti-CD3 (clone 500A2) antibody was utilized in the flow cytometry panel for T lymphocyte subset analysis. Cells were stained with fluorochrome-conjugated antibodies for 20 minutes at room temperature. After washing, samples were run on a FACSCalibur flow cytometer. Data analysis included compensation matrices derived from single-color controls. The protocol ensures minimal spectral overlap in multicolor setups.
Tags: 500A2 antibody mouse dose range; 500A2 mAb for flow cytometry

Deep metabolic profiling of immune cells by spectral flow cytometry reveals dynamic changes in mitochondrial function during activation
Gallego-Marin A, et al. (2025). iScience. 28(7):110123. doi: 10.1016/j.isci.2025.110123. PMID: 38945678
500A2 (AF700-conjugated) was employed for T cell phenotyping in spectral flow cytometry to track activation states. Isolated splenocytes were stained with a panel of antibodies including anti-CD3 (500A2) for 30 min at 4°C. Mitochondrial membrane potential was assessed using TMRE dye concurrently. Data were collected on a Cytek Aurora spectral cytometer and unmixing performed with SpectroFlo software. Activation-induced metabolic shifts were quantified in CD3+ populations over time.
Tags: 500A2 monoclonal antibody; anti-mouse CD3e 500A2

For more references about anti-mouse CD3e antibody (500A2), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry