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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: KT3.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: KT3) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant rat monoclonal antibody (clone: KT3) specifically binds to the mouse CD3e.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: KT3.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-mouse CD3e monoclonal antibody (clone: KT3) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant rat monoclonal antibody (clone: KT3) specifically binds to the mouse CD3e.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant Rat IgG2a Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: KT3.
Isotype: Rat IgG2a Kappa.
Source: The site-directed biotinylated anti-mouse CD3e monoclonal antibody (clone: KT3) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant rat monoclonal antibody (clone: KT3) specifically binds to the mouse CD3e.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Anti-CD3 therapy permits regulatory T cells to surmount T cell receptor-specified peripheral niche constraints
Nishio J, et al. J Exp Med. 2010 Aug 30;207(9):1879-89. doi: 10.1084/jem.20100205. PMID: 20679403
For anti-CD3 treatment, mice were injected i.v. with 3 ug/g BW of anti-CD3 mAb (clone;KT3; protein G purified) for 5 consecutive days. For T reg depletion in BR.Foxp3 DTR mice, 50 ug/g BW of DT was administered at days 1, 2, 4, 6, and 8). For blocking studies, 75 ug of anti-IL-2 (clone; JES6-1A12; BioLegend), anti-IL-7Ra (clone; A7.R34.2.2; protein G purified), and anti-IL-15 (clone; Al0.3; eBioscience) were injected ip for 6 consecutive days, starting on the same day as anti-CD3. 0.5mg of anti-TGFB (clone; 2G7; protein G purified) was administered every other day for 2 wk. For in vivo BrdU incorporation, 0.01 mg/g BW of BrdU was injected i.p., 6 h before euthanasia.
Tags: function of anti-mouse CD3e KT3; bioactivity of anti-mouse CD3e KT3
Site-directed multivalent conjugation of antibodies to ubiquitinated payloads
el Hebieshy AF, et al. Nat Biomed Eng. 2025 Jul;9(7):1101-1116. doi: 10.1038/s41551-024-01342-z. PMID: 40204992
For the initial conjugation reaction, the KT3 hybridoma-derived anti-mouse CD3 Fab-Ub(K48R)don, the chemically synthesized Ubacc-ΔGG carrying an N-terminal rhodamine fluorophore (Rho-Ubacc-ΔGG), in combination with recombinant E1 and the lysine-48 (K48)-specific ubiquitin E2–E3 fusion protein gp78RING-Ube2g2 (ref. 26) were chosen (Fig. 1b). The following hybridoma cell lines were modified for the stable expression of ubi-tagged antibodies or antibody fragments: KT3 (kindly provided by Dr Ramon Arens (LUMC, the Netherlands)), NLDC-145 (ATCC HB-290) and TA99 (ATCC HB-8704). KT3 and EL4 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 7.5% FCS.
Tags: anti-mouse CD3e antibody KT3; anti-CD3e (clone KT3)
Anti-CD3 treatment up-regulates programmed cell death protein-1 expression on activated effector T cells and severely impairs their inflammatory capacity
Wallberg M, et al. Immunology. 2017 Jun;151(2):248-260. doi: 10.1111/imm.12729. PMID: 28211040
The non-Fc receptor-binding anti-mouse CD3 antibody (agly-anti-CD3) was generated through genetic engineering in the Waldmann Laboratory, University of Oxford. It consists of the antigen-binding variable domain of the anti-mouse CD3 KT3 clone fused to mutated (non-FcR binding) human IgG1 heavy and κ light chains. Chimeric KT3-1.1 aglycosyl IgG1 antibody mRNA was prepared from the cells of the hybridoma KT3-1.1 and cDNA was prepared by anchor-tailed PCR.
Tags: clone KT3 of anti-mouse CD3e; KT3 monoclonal antibody
For more references about anti-mouse CD3e antibody (KT3), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry
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