Mouse IgG1 Isotype Control Antibody

Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions. 

Please contact us to ask for a quote for the Fc silenced mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and DYKDDDDK-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG1 isotype control and Fc-slient mutants are available.

PA007126: In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in CHO cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007135: In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody (mIgG1 D265A Isotype Control)

The in vivo grade recombinant mouse IgG1-D265A isotype control antibody (mIgG1 D265A isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant mouse IgG1 isotype control and mutants are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

References of Mouse IgG1 Isotype Control Antibody:

Self-Renewal and Toll-like Receptor Signaling Sustain Exhausted Plasmacytoid Dendritic Cells during Chronic Viral Infection.
Macal, M., et al. Immunity. 2018 Apr;48(4):730-744.e5. doi: 10.1016/j.immuni.2018.03.020. PMID: 29669251
WT mice were treated with 500μg/mouse intraperitoneally (i.p.) with neutralizing antibody (nAb) to IFNAR or Anti-Mouse IgG1 Isotype Control on days −1 and 0 post-LCMV Cl13-infection. ...Although characterization of T cell exhaustion has unlocked powerful immunotherapies, the mechanisms sustaining adaptations of short-lived innate cells to chronic inflammatory settings remain unknown. ...During murine chronic viral infection, we found that concerted events in bone marrow and spleen mediated by type I interferon (IFN-I) and Toll-like receptor 7 (TLR7) maintained a pool of functionally exhausted plasmacytoid dendritic cells (pDCs). ...In the bone marrow, IFN-I compromised the number and the developmental capacity of pDC progenitors, which generated dysfunctional pDCs. ...On the other hand, pDC functional loss was mediated by TLR7, leading to compromised IFN-I production and resistance to secondary infection.
Tags: activity of Mouse IgG1 Isotype Control; Mouse IgG1 Isotype Control

Control of murine cytomegalovirus infection by γδ T cells.
Sell, S., et al. PLoS Pathog. 2015 Feb 6;11(2):e1004481. doi: 10.1371/journal.ppat.1004481. PMID: 25658831
In addition neutralizing antibodies against IFNγ (clone XMG1.2), IL-17 (clone 17F3) or their isotype controls (rat IgG1 and mouse IgG1 respectively) were administered. ...All in vivo antibodies were purchased from BioXCell. ...For analysis of whole blood lymphocytes 2 drops of blood were taken from the tail vein in heparinized tubes. ...Infections with cytomegalovirus (CMV) can cause severe disease in immunosuppressed patients and infected newborns. ...Expansion of γδ T cells has been observed during human CMV (HCMV) infection in the fetus and in transplant patients with HCMV reactivation but the protective function of γδ T cells under these conditions remains unclear.
Tags: human IgG1 recombinant isotype control; Mouse IgG1 Isotype Control antibody for cancer research

Nasopharyngeal lymphatic plexus is a hub for cerebrospinal fluid drainage.
Yoon, J.-H., et al. Nature. 2024 Jan;625(7996):768-777. doi: 10.1038/s41586-023-06899-4. PMID: 38200313
Aged Prox1-GFP mice received i.p. injection of 200 µg of anti-interferon type I signalling blocking antibody or 200 µg mouse IgG isotype control every 72 h for 6 weeks. The sample was then cut along the sagittal plane in half with a blade and images were captured without fixation using a fluorescence stereo zoom microscope with a Plan-Neofluar Z ×1.0 objective lens with a HE-GFP or Cy3 filter. ...For immunofluorescence staining analyses, after right atrium puncture, ice-cold PBS was perfused into the left ventricle to remove blood. ...For tissue clearing, heads perfused with 4% PFA were immersed further in 4% PFA overnight at 4 °C for post-fixation, washed with PBS and incubated in CUBIC-L solution (TCI, T3740) with daily change for 7 days at 37 °C. ...After tissue clearing and PBS washing, the samples were subjected to decalcification, immunofluorescence staining and imaging. ...The samples were incubated in 5% normal donkey serum for 1 h at room temperature.
Tags: Mouse IgG1 Isotype Control antibody for mouse tumor model; Mouse IgG1 Isotype Control for animal model

β2 integrins impose a mechanical checkpoint on macrophage phagocytosis.
Settle, A. H., et al. Nat Commun. 2024 Sep 18;15(1):8182. doi: 10.1038/s41467-024-52453-9. PMID: 39294148
Phagocytosis is an intensely physical process that depends on the mechanical properties of both the phagocytic cell and its chosen target. ...Here, we employed differentially deformable hydrogel microparticles to examine the role of cargo rigidity in the regulation of phagocytosis by macrophages. ...Using phosphoproteomics, we identified β2 integrins as critical mediators of this mechanically regulated phagocytic switch. ...Macrophages maintain homeostasis in multicellular organisms by phagocytosing microbes, cell corpses, and biomolecular debris. ...Receptor engagement induces dramatic reorganization of filamentous (F)-actin at the interface, which shapes the overlying plasma membrane into a phagocytic cup that engulfs the cargo, ultimately leading to its internalization in an acidifying phagosome.
Tags: Mouse IgG1 Isotype Control for cancer research; Mouse IgG1 Isotype Control for mouse tumor model

Enhancing anti-EGFRvIII CAR T cell therapy against glioblastoma with a paracrine SIRPγ-derived CD47 blocker.
Martins, T. A., et al. Nat Commun. 2024 Nov 9;15(1):9718. doi: 10.1038/s41467-024-54129-w. PMID: 39521782
Cells were then treated for 30 min at 4 °C with 50 µL per well of 10 µg/mL of InVivoMAb anti-human CD47 or InVivoMAb mouse IgG1 isotype control or conditioned-media from 24 h-rested, antigen-naïve aEGFRvIII or aEGFRvIII-SGRP CAR T cells seeded at a density of 1 × 106 cells per mL of unsupplemented RPMI. ...The following treatments and controls were administered i.t. in a volume of 4 µL on days 7 and 14: vehicle (PBS), antibody isotype (InVivoMAb mouse IgG1 isotype control, clone MOPC-21; 5 µg), aCD47 (InVivoMAb anti-human CD47, clone B6.H12; 5 µg), aCD19 CAR (5 × 105 cells), aCD19-SGRP CAR (5 × 105 cells), aEGFRvIII CAR (5 × 105 cells), aEGFRvIII CAR + aCD47 (5 × 105 cells and 5 µg, respectively) or aEGFRvIII-SGRP CAR (5 × 105 cells). ...Mice were monitored for clinical signs until day 90, upon which all remaining survivors were either euthanized or assigned to a tumor rechallenge experiment. ...GBM engraftment and growth were monitored by BLi. Mice implanted i.c. with EGFRvIII-mosaic tumors were subjected to dual BLi with specific substrates to monitor the growth of the EGFRvIII+ and EGFRvIII- tumor fractions. ...For spectral FC analyses, mice were euthanized by CO2 suffocation, and brain regions were immediately harvested into ice-cold HBSS.
Tags: function of Mouse IgG1 Isotype Control antibody; Mouse IgG1 Isotype Control antibody mouse dose range

For more references about Mouse IgG1 Isotype Control Antibody please contact our scientific support team with message@sydlabs.com.

Questions and Answers:

Question: MOPC21 or MOPC-21 (hybridoma clone number, isotype: mouse IgG1, kappa) is widely used as a mouse IgG1 isotype control produced directly by hybridoma or in the recombinant formats. Is your mouse IgG1 isotype control also Mouse IgG1, kappa monoclonal (MOPC-21) - isotype control?
Answer: Similar to mouse IgG1 isotype control (MOPC-21), no matter purified from hybridoma or produce by the recombinant method, our recombinant mouse IgG1 isotype controls (catalog # PA007126 and PA007135) has unknown binding specificity. But our mouse IgG1 isotype controls are not from the MOPC-21 hybridoma or its antibody sequences though we also produce and sell the recombinant mouse (MOPC-21) mAb IgG1 isotype control as well as Fc mutants. Please contact us and ask for the clone MOPC-21 derived recombinant mouse IgG1-k isotype controls if you are interested.

Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage of the in vitro method. First, we do not use mouse ascites for antibody production. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies with the chemically defined medium. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation as the primary antibody in the application.

Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate, if there is any, non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls against KLH, TNP, DNP,  GST, HEL, His-tag, DYKDDDDK, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit