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Mouse IgG2c Isotype Control Antibody

mIgG2c Isotype Control, Mouse IgG2c LALAPG Isotype Control Mutant

Catalog No. Product Name Size List Price (US$) Quantity
PA005171 In Vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody 1 mg 160.00
PA005171 In Vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody 5 mg 300.00
PA005171 In Vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody 25 mg 800.00
PA005171.05 In Vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody, Endotoxin 0.05 EU/mg 5 mg 450.00
PA005171.05 In Vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody, Endotoxin 0.05 EU/mg 25 mg 1200.00
PA007141 In Vivo Grade Recombinant Mouse IgG2c-L234A L235A P329G (LALAPG) Isotype Control Antibody 1 mg 150.00
PA007141 In Vivo Grade Recombinant Mouse IgG2c-L234A L235A P329G (LALAPG) Isotype Control Antibody 5 mg 300.00
PA007141 In Vivo Grade Recombinant Mouse IgG2c-L234A L235A P329G (LALAPG) Isotype Control Antibody 25 mg 800.00
PA007141.05 In Vivo Grade Recombinant Mouse IgG2c-L234A L235A P329G (LALAPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg 5 mg 450.00
PA007141.05 In Vivo Grade Recombinant Mouse IgG2c-L234A L235A P329G (LALAPG) Isotype Control Antibody, Endotoxin 0.05 EU/mg 25 mg 1200.00
Description

The mouse IgG2c antibody is produced in certain inbred strains such as C57BL/6, C57BL/10, SJL, and NOD, which does not express the mouse IgG2a antibody, and vice versa. If one uses any of the mouse strains for animal research, it is better to use the IgG2c antibodies rather than the IgG2a antibodies. There are more and more IgG antibody formats with Fc engineered. The format with the Fc silenced, Fc silent, or Fcs with silenced effector function is the most popular. Thus, the wild type mouse IgG2c isotype control and the Fc silenced mouse IgG2c isotype control mutants should be used in such an application.

PA005171: In Vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody (mIgG2c Isotype Control)

The in vivo grade recombinant mouse IgG2c isotype control antibody (mIgG2c isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG2c, kappa.
Applications: an isotype-matched negative control for mouse IgG2c kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007141: In Vivo Grade Recombinant Mouse IgG2c-L234A L235A P329G Isotype Control Antibody (mIgG2c LALAPG Isotype Control)

The in vivo grade recombinant mouse IgG2c-L234A L235A P329G (mIgG2c LALAPG, Fc silenced) isotype control antibody (mIgG2c LALAPG isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG2c, kappa.
Applications: an isotype-matched negative control for mouse IgG2c LALAPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

Citations of Syd Labs Mouse IgG2c Isotype Control Antibodies:

Lymphatic pumping technique in mice alters blood parameters and metastatic melanoma in an age-dependent manner
Kopchick JJ,et al.Exp Biol Med (Maywood). 2026 Mar 30;251:10850. doi: 10.3389/ebm.2026.10850. PMID: 41982211; PMCID: PMC13071501.
“The experimental design of the four in vivo experiments reported in this study is shown in …… In addition to the sham and LPT treatments, the mice were treated with anti-mouse programmed cell death protein 1 (PD1) antibody (#PA007163.m2cLA, Syd Labs) or an isotype control antibody (#PA007141, Syd Labs). Four treatments of 200 μg of antibody diluted in 100 μL of PBS (2 mg/mL) were administered intraperitoneally between days 9 and 21 of the experiment. The anti-PD1 dosing regimen of 200 ug per injection was selected based on established murine immunotherapy protocols rather than allometric scaling from human doses, consistent with prior studies demonstrating biological efficacy at this dose [21, 22]. Mice were divided into four treatment groups: group 1 received sham treatment and isotype control antibodies; group 2 received LPT and isotype control antibodies; group 3 received sham treatment and anti-PD1 antibodies; and group 4 received LPT treatment and anti-PD1 antibodies. The cancer cells were injected on experimental day 0, treatments started on day 8, ended on day 22, and the mice were euthanized with carbon dioxide inhalation and on day 25.”
Tags: activity of Mouse IgG2c-L234A L235A P329G (LALAPG) isotype control; Mouse IgG2c-L234A L235A P329G (LALAPG) isotype control

For more references about mouse IgG2c isotype control antibodies please contact our scientific support team with message@sydlabs.com.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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