De novo antibody sequencing to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.
Sample requirement: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/mL).
Fast turnaround time: 2-3 months per antibody, but may require more time depending on the complexity of analysis.BS052: HRP Conjugation Service
1. Highly efficient process to conjugate HRP to clients' proteins and antibodies
2. Amine-reactive HRP achieves the highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. HRP enzyme activity is ~150 units/mg
5. Fast turnaround time: 3-5 daysBS077: Fluorophore Conjugation Service
1. Highly efficient process to conjugate all commercially available fluorophore to clients' proteins and antibodies
2. The highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. Fast turnaround time: 3-5 daysBS076: Biotin Conjugation Service
1. Highly efficient process to conjugate biotin to clients' peptides, proteins or antibodies
2. The highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. Fast turnaround time: 3-5 daysBS054: Magnetic Bead Conjugation Service
Highly efficient process to conjugate magnetic beads to clients' peptides, proteins, and antibodies: the ratio is 20 ug protein or antibody per mg magnetic microbeads in the conjugation reaction; about 6 ug protein or antibody would be conjugated onto the beads at a concentration of 6 ug antibody per mg magnetic beads.
Fast turnaround time: 3-5 days.- Reliable results. Through development of genome-wide qPCR primers, we have successfully tested over 30,000 genes in qPCR assays. Relative and absolute analysis of gene expression by qPCR is available for all known genes in any organism.
- Save money. No need to buy real-time PCR instruments or reagents. Our reverse transcription and qPCR reagents are used to save clients' cost.- Reliable results. Through development of genome-wide qPCR primers, we have successfully tested over 30,000 genes in qPCR assays. Relative and absolute analysis of gene expression by qPCR is available for all known genes in any organism.
- Save money. No need to buy real-time PCR instruments or reagents. Our reverse transcription and qPCR reagents are used to save clients' cost.Custom Ilama and alpaca monoclonal antibody development (VHH, sdAb or nanobody) by Ilama or alpaca immunization and phage display screening.
Production of camel, Ilama or alpaca VHH in E.coli or VHH-Fc in HEK 293 cells.
Fast turnaround: 4-6 months once the antigen is available.Our complete custom Adeno-associated Virus (AAV) production services include: 1) AAV plasmid vector construction; 2) AAV packaging; 3) AAV purification by iodixanol or column chromatography; 4) AAV titering by qPCR.
We have always optimized our AAV production systems, including AAV plasmid vector design and construction and low endotoxin plasmid preparation, host cells for AAV packaging, cell culture medium and AAV packaging process, small and large scale AAV purification process.
Fast turnaround: 2-3 weeks.The most comprehensive murine hybridoma generation for therapeutic antibody services:
Genetic background: white, black, immunodeficiency, knock-out, and humanized mice; rat; hamster;
Antigens: proteins, peptides, DNA, cells expressing certain proteins.
All are done in US.