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Camelid Monoclonal Antibody Production Services

Custom Llama Antibody Development, Custom Alpaca Antibody Generation, Nanobody Production Service. sdAb
Catalog No. Product Name Size List Price (US$) Quantity
BS078 ELISA Guaranteed Camel Monoclonal Antibody Development Service 1 package Request

Syd Labs team has worked on camelid (camel, llama and alpaca) antibody development projects since 2013. VHH (nanobody, single domain antibody or sdAb) has been successfully used in diagnostics especially imaging, therapeutics (therapeutical antibodies, bispecific antibodies, CAR T, and ADC), manufacturing and research reagents. 

Procedure of camel, Ilama and alpaca single chain (VHH, nanobody or sdAb) antibody development:
Phase I: Immunization and boosts of 1 camel, Ilama or alpaca, and ELISA screening of camel, Ilama or alpaca polyclonal sera.
Phase II: RNA isolation, cDNA synthesis, PCR amplification, and construction of a phage display library of VHH, a single variable domain (VH) of a heavy chain antibody.
Phase III: Screening antigen-specific VHH by phage display. 4-6 potential clones will be identified and evaluated.
Phase IV: Molecular construction and production of 4-6 ELISA-positive His-tagged VHH protein in E.coli.

You provide: Five mg of soluble protein (concentration > 0.5 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, VHH library screening, and binding confirmation.

* DNA sequences of 4-6 antigen-specific VHH;
* Purified His-tagged VHH;
* Remaining antigens;
* ELISA results.

3-4 months.

Camel, alpaca, and llama VHH antibody humanization service is available upon request.

The prices will be adjusted for the following requirements:

a. Protein: DNA subcloning +$400, bacterial expression and purification +$2,500;
b. Additional boost immunization for higher ELISA titer of the antisera +$300/injection.

We also provide other monoclonal antibody development services:

Mouse monoclonal antibody development services
Rat monoclonal antibody development services
Rabbit monoclonal antibody development services
Human monoclonal antibody development services

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