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Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
De novo protein sequencing of monoclonal antibodies (mAbs) is required when neither the original cell line nor mRNA/cDNA is available, or when characterization of post-translational modifications is needed to verify antibody integrity and effectiveness.
Sample requirements:
Prerequisites- services required to determine feasibility of de novo antibody sequencing:
A. Intact Mass Analysis by LC-MS;
B. RP-HPLC Analysis;
Sample size: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/ml);
Formulation: freeze-dried or dissolved in 1X PBS or equivalent buffers;
Purity: > 95%;
Filtration: filtered with 0.22 um filters.
Information required from clients:
A. Source of antibody (e.g., mouse ascites or commercial mAb);
B. Isotype (if known);
C. Formulation (buffer type, pH, molar concentration, any additives, and protein concentration);
D. Any information on solubility and/or stability of the test article.
Deliverable:
A. Primary amino acid sequences of variable regions (VH and VL) of antibody heavy and light chains;
B. Purified 3 mg scale transient production of recombinant monoclonal antibodies;
C. Study report.
We also provide the following antibody sequencing services:
Cloning and Sequencing Heavy- and Light-chain Variable Regions (VH and VL) of Hybridoma or Clonal B Lines
Single B Cell Antibody Cloning and Sequencing Service
Sequence Liability Identification Service
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