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Recombinant rabbit IgG isotype control antibody for use as an isotype-matched negative control in immunoassays employing rabbit IgG primary antibodies.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The recombinant rabbit IgG isotype control antibody was produced in CHO cells.
Specificity / Sensitivity: This recombinant rabbit IgG isotype control antibody is intended for use as a negative control alongside rabbit IgG antibodies directed against the target of interest. Background reactivity should be evaluated in the specific sample and assay system.
Isotype: Rabbit IgG.
Applications: an isotype-matched negative control for rabbit IgG primary antibodies. Potential applications include immunoprecipitation (IP), co-immunoprecipitation (Co-IP), chromatin immunoprecipitation (ChIP), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), flow cytometry, ELISA, and Western blotting (WB). Suitability for each application should be established experimentally.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Recommended Usage: Use at the same final mass concentration as the test antibody rather than at the same dilution factor. For immunoprecipitation-based assays, use a matched antibody amount per reaction. Optimize the working concentration for each assay, as excessive control antibody may increase background.
Shipping: The recombinant rabbit IgG isotype control antibodies are shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
Syd Labs recombinant rabbit IgG isotype control antibody is designed to help assess nonspecific background in experiments using rabbit IgG primary antibodies. It provides an immunoglobulin-matched control for evaluating background associated with Fc receptor interactions, nonspecific adsorption, and other antibody-dependent interactions under the assay conditions.
Rabbit immunoglobulin G (IgG) is an approximately 150 kDa antibody composed of two identical heavy chains and two identical light chains linked by disulfide bonds. The antigen-binding regions recognize specific epitopes, whereas the Fc region interacts with Fc receptors and other immunoglobulin-binding proteins. Rabbit IgG antibodies are widely used in protein detection, cellular imaging, and immunoprecipitation.
Rabbit IgG isotype control antibodies help distinguish target-associated signals from nonspecific antibody binding. Syd Labs recombinant rabbit IgG isotype control antibody provides a recombinant-format negative control for experiments using rabbit IgG primary antibodies. The control should be used at the same final antibody concentration and under the same experimental conditions as the test antibody. For conjugated primary antibodies, the control should also carry a matching label with a comparable degree of labeling.
Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody
In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)
Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit
Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit
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