The most comprehensive rabbit monoclonal antibody production services: custom production of rabbit monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; rabbit monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Syd Labs team has successfully developed a large number of high specificity and high affinity rabbit monoclonal antibodies against a wide range of difficult targets such as small molecules, antibody idiotype, and antigens non-immunogenic in murine, camel or Ilama.
Fast turnaround: 18 weeks once the antigen is available.Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.
A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.
Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.
- Any gene;
- Any host cell, including primary cell;
- Significantly improved efficiency of changing protein expression level compared to non-viral DNA transfection methods.
Fast turnaround:
Clonal stable cell line generation: 12-14 weeks.
Cell pool generation: 8-10 weeks.Syd Labs has successfully developed human and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as drug development and diagnostics.
Fast turnaround: 8-10 weeks once the polyclonal sera, PBMC, and antigen is available.Experienced scientists working on antigen and antibody production, antibody engineering, lentivirus, and cell line development services for decades. >90% success rate of stable cell line generation.
Fast turnaround:
Antigen (peptide or protein) production: 3-6 weeks.
Mouse monoclonal antibody production: 20-24 weeks.
Screening phage or yeast libraries for fully human antibodies: 8-12 weeks.
Sequencing hybrioma antibody: 1-3 weeks.
Chimeric antibody production: 3-4 weeks.
Antibody humanization: 8-10 weeks.
Construction of CAR cassette, lentivirus packaging and production: 4-5 weeks.
T-cell activation, transduction, and CAR T-cell expansion: 4-5 weeks.
Confirmation of CAR expression, IFN-gamma ELISA, and cytotoxicity assay of transduced and expanded T-cells: 2 weeks.Our retrovirus production services include: 1) construction of a retrovirus plasmid vector for expression or silencing of a gene; 2) retrovirus packaging; 3) retrovirus purification; 4) retrovirus titration.
We have always optimized our retrovirus production systems, including retrovirus plasmid construction and low endotoxin plasmid preparation, host cells for retrovirus packaging, cell culture medium and retrovirus packaging process, small and large scale retrovirus purification process.
Fast turnaround: 2-3 weeks.Our complete custom lentivirus production services include: 1) lentivirus vector construction for expression or silencing of a gene; 2) lentivirus packaging; 3) lentivirus purification; 4) lentivirus titering.
We have always optimized our lentivirus production systems, including lentivirus plasmid construction and low endotoxin plasmid preparation, host cells for lentivirus packaging, cell culture medium and lentivirus packaging process, small and large scale lentivirus purification process.
Fast turnaround: 2-3 weeks.We provide large selection of pre-made adenovirus and complete custom adenovirus production services from small crude scale to large purified scale, including: 1) construction of an adenovirus vector for expression or silencing of a gene; 2) generation of adenovirus; 3) amplification and purification of adenovirus.
Our adenovirus production team has constructed, packaged, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An optimized technology is used to simplify and accelerate adenoviral vector construction for researchers studying gene expression or gene silencing in mammalian systems.
Fast turnaround: 2-3 weeks.Syd Labs determines the titer of lentiviruses and retroviruses (provided by customer or produced by Syd Labs) using HT1080 cells.
Fast return: 2 weeks.
Syd Labs determines the titer of lentiviruses and retroviruses (provided by customer or produced by Syd Labs) using HT1080 cells.
Fast return: 2 weeks.