Anti-mouse CD16 / CD32 Monoclonal Antibodies

PA007389.r2b: In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Rat IgG2b Kappa (Clone: 2.4G2)

Recombinant rat IgG2b Monoclonal Antibody. 
Clone: 2.4G2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD16/CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 2.4G2) specifically binds to mouse CD16 and CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD16 and CD32 protein. Mouse Fc receptor blocking solution, mouse Fc receptor blocking reagent, and mouse Fc receptor blocking antibody.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007389.h1: In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Human IgG1 Kappa (Clone: 2.4G2)

Recombinant human IgG1 monoclonal antibody.
Clone: 2.4G2.
Isotype: Human IgG1 kappa.
Source: The anti-mouse CD16/CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 2.4G2) specifically binds to mouse CD16 and CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD16 and CD32 proteins. Mouse Fc receptor blocking solution, mouse Fc receptor blocking reagent, and mouse Fc receptor blocking antibody.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007389.m2aLA: In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Mouse IgG2a LALAPG Kappa (Clone: 2.4G2)

Recombinant mouse IgG2a monoclonal antibody with the Fc silent mutation.
Clone: 2.4G2.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse CD16/CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 2.4G2) specifically binds to mouse CD16 and CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD16 and CD32 proteins. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007389.mf: In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Mouse Fab fragment (Clone: 2.4G2)

Recombinant mouse Fab monoclonal antibody.
Clone: 2.4G2.
Isotype: Mouse Fab.
Source: The anti-mouse CD16/CD32 Fab monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse Fab monoclonal antibody (clone: 2.4G2) specifically binds to mouse CD16 and CD32.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD16 and CD32 proteins. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The recombinant 2.4G2 antibody binds to mouse CD16 (FcγIII) and CD32 (FcγII) receptor proteins. The major component of some commercial Fc block reagents is the 2.4G2 antibody. The rat, mouse, and human IgG Fc binds to mouse CD64 (FcγI). Thus, the recombinant 2.4G2 antibodies can be used to block non-specific binding of IgGs to mouse Fc receptors in flow cytometry, immunohistochemistry, immunoprecipitation, and blocking assays. Customers may choose different IgG isotypes for their own applications.  

The most popular mouse Fc blocking reagents include:
Anti-Mouse CD16/CD32 (Clone 2.4G2): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: for general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Purified rat IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions. 

Best choice of mouse Fc blocking reagents based on your application are:
For flow cytometry: anti-mouse CD16/CD32 (Clone 2.4G2).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of Anti-mouse CD16 / CD32 Monoclonal Antibodies:

Metabolic deficiencies underlie reduced plasmacytoid dendritic cell IFN-I production following viral infection.
Greene, T. T., et al. Nat Commun. 2025 Feb 7;16(1):1460. doi: 10.1038/s41467-025-56603-5. PMID: 39920132
Cells were pre-incubated with CD16/CD32 Fc block (BD Biosciences, Catalog #: 553141) for 10 min prior to surface staining. ...Type I Interferons (IFN-I) are central to host protection against viral infections, with plasmacytoid dendritic cells (pDC) being the most significant source, yet pDCs lose their IFN-I production capacity following an initial burst of IFN-I, resulting in susceptibility to secondary infections. ...The underlying mechanisms of these dynamics are not well understood. ...Mechanistically, we identify lactate dehydrogenase B (LDHB) as a positive regulator of pDC IFN-I production in mice and humans; meanwhile, LDHB deficiency is associated with suppressed IFN-I production, pDC metabolic capacity, and viral control following infection. ...Our work thus identifies a mechanism for balancing immunity and pathology during viral infections, while also providing insight into the highly preserved infection-driven pDC inhibition.
Tags: anti-mouse CD16/CD32 2.4G2; anti-mouse CD16/CD32 2.4G2 in vivo

An LNP-mRNA vaccine modulates innate cell trafficking and promotes polyfunctional Th1 CD4+ T cell responses to enhance BCG-induced protective immunity against Mycobacterium tuberculosis.
Lukeman, H., et al. EBioMedicine. 2025 Mar;113:105599. doi: 10.1016/j.ebiom.2025.105599. PMID: 39955975
If undergoing tetramer staining, unstimulated single cell suspensions were initially incubated with tetramer diluted 1:150 in cRPMI containing Fc-block for 45 min at 37 °C, 5% CO2. ...We have developed a lipid nanoparticle (LNP)-mRNA vaccine, termed mRNACV2, encoding for the M. tuberculosis CysVac2 fusion protein, which we have previously formulated as an adjuvanted subunit vaccine. ...This LNP-mRNA vaccine was administered intramuscularly to female C57BL/6 mice as a standalone vaccine or as booster to BCG to assess immunogenicity and efficacy of the construct. ...These findings of a protective LNP-mRNA vaccine for TB highlight the potential of the LNP-mRNA platform for TB control and support further research to facilitate translation to humans. ...Despite the existence of curative antibiotics and a licenced vaccine, Mycobacterium tuberculosis remains a major burden on global health.
Tags: anti-mouse CD16/CD32 2.4G2 in animal model; anti-mouse CD16/CD32 2.4G2 antibody in animal model

An mpox quadrivalent mRNA vaccine elicits sustained and protective immunity in mice against lethal vaccinia virus challenge.
Li, E., et al. Emerg Microbes Infect. 2025 Dec;14(1):2447619. doi: 10.1080/22221751.2024.2447619. PMID: 39745170
The cells were blocked for 15 min in PBS supplemented with 1% anti-CD16/CD32 and then incubated with the following monoclonal antibodies for 30 min at 4°C: Fixable Viability Stain 700, PerCP-Cy5.5 Rat Anti-Mouse CD8a, APC-Cy7 Hamster Anti-Mouse CD3e, and BV421 Rat Anti-Mouse CD107a. ...Assessing the long-term efficacy of MPXV vaccine candidates is crucial for the global response to the ongoing mpox epidemic. ...Built upon our previous study of the mpox quadrivalent mRNA vaccine, herein we reported that MPXV-1103 could elicit sustained humoral and cellular immunity in mice, including the induction of MPXV A35/B6/A29/M1-specific IgG antibodies, VACV neutralizing antibodies and activated cytotoxic CD8+T cells, which provides 100% protection against lethal VACV challenge even at 280 days after the first vaccination. ...The longevity of the presence of antibodies demonstrates the effectiveness of VACV as a smallpox vaccine in providing long-lasting immunity. ...Previously, we developed an mpox quadrivalent mRNA vaccine (termed MPXV-1103) expressing full-length B6, A35, A29 and M1 proteins with three flexible linkers in a single sequence.
Tags: anti-mouse CD16/CD32 2.4G2 in cancer research; anti-mouse CD16/CD32 2.4G2 mAb in cancer research

Enhancing immunotherapy efficacy in colorectal cancer: targeting the FGR-AKT-SP1-DKK1 axis with DCC-2036 (Rebastinib).
Chen, X., et al. Cell Death Dis. 2025 Jan 9;16(1):8. doi: 10.1038/s41419-024-07263-8. PMID: 39788945
For flow cytometry, the antibodies used were: fixable viability stain 780, CD45, CD3, CD8, CD4, CD69, and purified rat anti-mouse CD16/CD32. ...This research demonstrates that DCC-2036 (Rebastinib), a potent third-generation tyrosine kinase inhibitor (TKI), effectively suppresses tumor growth in colorectal cancer (CRC) models with functional immune systems. ...The findings underscore the capacity of DCC-2036 to enhance both the activation and cytotoxic functionality of CD8+ T cells, which are crucial for facilitating anti-tumor immune responses. ...Moreover, our findings identify FGR as a pivotal modulator that influences DKK1 expression via the PI3K-AKT-SP1 signaling cascade. ...Consequently, targeting the FGR-AKT-SP1-DKK1 pathway with DCC-2036 could potentiate immunotherapy by enhancing CD8+ T cell functionality and their tumor infiltration.
Tags: anti-mouse CD16/CD32 2.4G2 mAb in mouse tumor model; function of anti-mouse CD16/CD32 2.4G2 mAb

For more references about Anti-mouse CD16 / CD32 Monoclonal Antibodies please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)

Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry