Random mutagenesis based on PCR and other methods.
The standard Western Blot protocol will be used to detect protein expression in solution, cell or tissue samples.
- Ion-Exchange HPLC (IEX-HPLC) for separation of samples based on differences in their surface charge.
Sample requirement: 100-200 ug.
Fast Turnaround time: 1 - 2 weeks. - Positive monoclonal hybridoma cell lines producing antibodies for designated in vitro functions.
- Positive monoclonal hybridoma cell lines producing antibodies for ELISA, WB, IP, IF, ICC, IHC, or FACS
- Normal-phase chromatography (NP-HPLC) is an analytical technique that is used to separate molecules on the basis of differences in the strength of their interaction with a polar stationary phase.
Sample requirement: 100-200 ug.
Fast turnaround time: 2 - 3 weeks. - Reversed-phase chromatography (RP-HPLC) is an analytical technique that separates molecules on the basis of differences in their hydrophobicity.
Sample requirement: 50-100 ug.
Fast turnaround time: 1 - 2 weeks. - We will link two approved anti-tumor antibiotics to your purified monoclonal antibody using our proprietary conjugation method. The anti-tumor agent will remain bound to the monoclonal antibody and inactive but will be released and activated by reduction once the immunocomplex binds to the cell surface antigen and internalized.
- Our areas of expertise in Immunohistochemistry (IHC) include:
* Background literature research
* Sourcing of optimal antibodies
* Antibody optimization
* Protocol development
* Routine IHC staining (manual and automated)
* Slide analysis, including digital microscopy imaging, digital whole slide scanning, staining quantification, pathological scoring/analysis, and report generation. Fast return: 8 days for standard site-directed mutagenesis;
Point mutations, deletions, or insertions of several amino acids can be performed in a single mutagenesis reaction. The same price is charged for incorporating several mutations, as long as the changes are localized and can be introduced in a single reaction. The same price is charged no matter the size of gene is.