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Services for synthesis, detection, identification, separation, purification, production, quantification, and modification of oligonucleotides, RNA, DNA, peptides, and proteins, such as peptide synthesis, gene synthesis, DNA cloning and subcloning, protein expression, custom antibody development, cell line generation, cell and tissue culture, and cell-based assays.
    90 Services
  • One-stop sequence optimization, gene synthesis, and DNA subcloning services.
    Various bacterial expression vectors developed by ourselves or our partners.

    Fast turnaround: 6-7 weeks.

    We have a lot of experience in dealing with difficult-to-express proteins.
    While the success rate of stable cell line generation is highly dependent on the target genes and Syd Labs can not guarantee 100% success rate, our in-house data show a success rate greater than 90%.

  • - Any gene;
    - Any host cell, including primary cell;
    - Significantly improved efficiency of changing protein expression level compared to non-viral DNA transfection methods.

    Fast turnaround:

    Clonal stable cell line generation: 12-14 weeks.
    Cell pool generation: 8-10 weeks.

  • Experienced scientists working on protein engineering, expression, and purification, and cell line development services for decades. >90% success rate of stable cell line generation.

    Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins.

    Fast turnaround:

    1 week for synthesizing a gene for expressing the target protein.
    1 week for molecular construction of the gene.
    5-6 weeks for generation of suspension CHO stable cell lines expressing the target protein.
    5-6 weeks for additional services included in the premium package.

  • 100000 cells per hybridoma are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL). Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

    Fast turnaround:

    * 2-3 weeks (1-2 weeks for express service) for cloning and sequencing antibody variable regions (VH and VL) of hybridoma or clonal B cells. An industry record: 4 days.
    * (Optional but highly recommended) 3-4 weeks for molecular construction, transient expression, and protein A purification of recombinant IgG or scFv-Fc from HEK 293 cells.
    * (Optional but highly recommended) 1 week for assessment of binding affinity and specificity of recombinant IgG or scFv.

  • Fast turnaround (BS079A, B, and C):

    * 1-2 weeks for codon optimization and synthesizing antibody variable regions.
    * 1-2 weeks for molecular construction of recombinant scFv-Fc antibodies using E.coli.
    * 1-2 weeks for expressing and purifying recombinant scFv-Fc antibodies using HEK 293 cells.

    Protein A is used for recombinant antibody purification. Please request if multiple purification methods are required.

    * BS079D: 0.03 liter transient production, estimated yield 0.5 - 12 mg, average 1.5 mg, 2 weeks;
    * BS079E: 0.1 liter transient production, estimated yield 3 - 110 mg, average 14 mg, 2 weeks;
    * BS079F: 0.5 liter transient production, estimated yield 12 - 300 mg, average 75 mg, 2 weeks;
    * BS079G: 2 liter transient production, estimated yield 50 - 2000 mg, average 250 mg, 3 weeks;
    * BS079H: 10 liter transient production, estimated yield 120 - 5000 mg, average 1100 mg, 3 weeks.

  • Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.

    A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.

    Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.

  • Fast turnaround: 3-6 months with our proprietary optimization algorithm.

    Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.

  • Fast turnaround (BS046A, B, and C):

    * 1-2 weeks for codon optimization and synthesizing antibody variable regions.
    * 1-2 weeks for molecular construction of recombinant IgG antibodies using E.coli.
    * 1-2 weeks for expressing and purifying recombinant IgG antibodies using HEK 293 cells.

    Protein A is used for recombinant antibody purification. Please request if multiple purification methods are required.

    * BS046D: 0.03 liter transient production, estimated yield 0.5 - 10 mg, average 2 mg, 2 weeks;
    * BS046E: 0.1 liter transient production, estimated yield 2 - 50 mg, average 10 mg, 2 weeks;
    * BS046F: 0.5 liter transient production, estimated yield 10 - 250 mg, average 50 mg, 2 weeks;
    * BS046G: 2 liter transient production, estimated yield 40 - 2000 mg, average 200 mg, 3 weeks;
    * BS046H: 10 liter transient production, estimated yield 200 - 5000 mg, average 1000 mg, 3 weeks.

  • It is critical for single B cell antibody generation technologies to clone and sequence the antibody variable region (VH and VL) from antigen specific single or clonal B cells after detection and isolation of antigen specific B cells by flow cytometry-based single B cell sorting. Syd Labs has successfully developed human, rabbit, mouse, rat, and monkey monoclonal antibodies by cloning and sequencing the variable antibody regions of single antigen-specific mouse memory B cells or clonal B cells grown in culture.

  • Syd Labs generates scFv phage libraries from immunized or naive donors from several species. Additionally, various mutagenesis strategies can be used to design scFv phage libraries for affinity maturation of a given antibody or other optimization purposes. Our scFv phage library is generated with a goal of up to 1010 clones and quality controlled by sequencing 96 clones at random.

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