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Normal Rabbit IgG

Rabbit IgG Isotype Control; Normal Rabbit Immunoglobulin G; Rabbit IgG Negative Control

Catalog No. Product Name Size List Price (US$) Quantity
Description

PA006904: Normal Rabbit IgG

Isotype: Rabbit IgG.
Applications: an isotype-matched negative control for rabbit IgG primary antibodies. Potential applications include immunoprecipitation (IP), co-immunoprecipitation (Co-IP), chromatin immunoprecipitation (ChIP), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), flow cytometry, ELISA, and Western blotting (WB). Suitability for each application should be established experimentally.
Recommended Usage: Use normal rabbit IgG at the same final mass concentration as the rabbit IgG primary antibody, rather than simply using the same dilution factor. For immunoprecipitation assays, use the same antibody amount per reaction and maintain equivalent sample input, bead quantity, incubation, and washing conditions. Optimize the working concentration for each assay.

Shipping: The normal rabbit IgG is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

Background of Normal Rabbit IgG

Normal rabbit IgG is a polyclonal immunoglobulin preparation purified from normal rabbit serum. It is used as a negative control to help evaluate nonspecific background in assays performed with rabbit IgG primary antibodies.

Rabbit immunoglobulin G (IgG) is an approximately 150 kDa glycoprotein composed of two identical heavy chains and two identical light chains connected by disulfide bonds. Its antigen-binding regions recognize epitopes, while the Fc region interacts with Fc receptors and other immunoglobulin-binding proteins. Rabbit IgG antibodies are widely used in immunological and biochemical research.

Normal rabbit IgG is purified from the serum of non-immunized rabbits and contains a diverse population of IgG molecules. It serves as a negative control to help assess background arising from nonspecific antibody binding, Fc receptor interactions, and other protein–protein interactions. Syd Labs Normal Rabbit IgG is intended for use alongside rabbit IgG primary antibodies under matched experimental conditions. The control should be used at the same final antibody concentration as the test antibody to support interpretation of the experimental results.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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