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De novo Antibody Sequencing Service

De novo Protein Sequencing of Monoclonal Antibodies

Catalog No. Product Name Size List Price (US$) Quantity
BS057A De novo Antibody Sequencing Service (Full package, guaranteed results) 1 antibody 25000.00
BS057B De novo Antibody Sequencing Service (Mass Spec results and analysis only) 1 antibody 15000.00
Description

De novo protein sequencing of monoclonal antibodies (mAbs) is required when neither the original cell line nor mRNA/cDNA is available, or when characterization of post-translational modifications is needed to verify antibody integrity and effectiveness.

Sample requirements:

Prerequisites- services required to determine feasibility of de novo antibody sequencing:
A. Intact Mass Analysis by LC-MS;
B. RP-HPLC Analysis;
Sample size: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/ml);
Formulation: freeze-dried or dissolved in 1X PBS or equivalent buffers;
Purity: > 95%;
Filtration: filtered with 0.22 um filters.

Information required from clients:

A. Source of antibody (e.g., mouse ascites or commercial mAb);
B. Isotype (if known);
C. Formulation (buffer type, pH, molar concentration, any additives, and protein concentration);
D. Any information on solubility and/or stability of the test article.

Deliverable:

A. Primary amino acid sequences of variable regions (VH and VL) of antibody heavy and light chains;
B. Purified 3 mg scale transient production of recombinant monoclonal antibodies;
C. Study report.

We also provide the following antibody sequencing services:
Cloning and Sequencing Heavy- and Light-chain Variable Regions (VH and VL) of Hybridoma or Clonal B Lines
Single B Cell Antibody Cloning and Sequencing Service
Sequence Liability Identification Service

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