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EK Enzyme

Enterokinase, Enteropeptidase

Catalog No. Product Name Size List Price (US$) Quantity
BP000034-GD8 Recombinant Bovine Enterokinase, Light Chain 200 IU 98.00
BP000034-GD8 Recombinant Bovine Enterokinase, Light Chain 1000 IU 320.00


Enterokinase (EK) is a serine proteinase that exhibits specificity for the sequence (Asp)4-Lys. It is the physiological activator of trypsinogen. The native EK enzyme has a heavy chain (115 kDa) covalently linked to a light chain or catalytic subunit (35 kDa). It has been well documented that the enterokinase light chain (EK) is catalytically effective (1), and thus most studies are performed using EK.


1. A. Light, P. Fonseca, The preparation and properties of the catalytic subunit of bovine enterokinase, J. Biol. Chem. 259 (1984) 13195-13198.

BP000034-GD8: Recombinant Bovine Enterokinase, Light Chain

Source: Yeast-derived.
N-terminal sequence analysis: Ile801
Predicted molecular mass: 26.3 kDa
SDS-PAGE: 31 kDa
Unit Definition: 1 IU of Enterokinase (EK) will cleave 50 ug of test substrate to 95% completion in 16 hours or less at 25°C.
Unit Assay Condition: 20 mM Tris-HCl (pH 8.0 @ 25°C), 50 mM NaCl, 2 mM CaCl2, 25 ug of an MBP fusion protein test substrate and enzyme. Incubate at 23°C.
Formulation: a 0.2 um filtered solution in Tris, and NaCl.

Usage notes:
Suggested Reaction Conditions: Cleavage of an MPB-paramyosin-ΔSal fusion protein with an enterokinase site requires 1 IU per 50 ug.
Removal: Enterokinase will bind specifically to trypsin inhibitor agarose (e.g., Sigma T-0637).

Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70°C as supplied.

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