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Conditions of optimal endotoxin-free TEV protease performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
The TEV protease, also designated NIa, is 241 amino acids long and is the catalytic domain of the encoded potyvirus protein. Endotoxin-free TEV protease is effectively used with high specificity and with various pHs and ionic strengths as a preferable protease to cleave affinity tags from recombinant proteins. This TEV protease can be used either co-translationally or following purification in vitro.
BP4114: Recombinant endotoxin-free TEV protease
Source: E.coli-derived.
Recombinant endotoxin-free TEV protease is the sequence of TEV Protease (accession # NP_062908, amino acids Glu2039-Gln2279 (Ser2256Asn)) fused with a 6-His tag at the C-terminus.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant endotoxin-free TEV protease cleaves after Gln in the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln.
Formulation: 0.2 μM filtered solution.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.
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