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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal PNGase F Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Background
PNGase F from Elizabethkingia miricola (or Flavobacterium meningosepticum) catalyzes the cleavage of N-linked glycans from glycoproteins and glyopeptides. PNGase F has been widely used in combination with other glycosidases for proteomic and biochemical analysis. Various masss spectrometry methods, such as MALDI-TOF, and in vitro biochemical assays following deglycosylation catalyzed by PNGase F are used to identify the presence of N-linked oligosaccharides on glycoproteins and glyopeptides, and to analyze their importance for structure and function of glycoproteins and glyopeptides.
References:
1. Mann, A.C., Self, C.H., and Turner, G.A. (1994) A general method for the complete deglycosylation of a wide variety of serum glycoproteins using peptide-N-glycosidase-F. Glycoconjugate Journal 1, 253–61.
BP004089-GD29: Recombinant PNGase F Enzyme (glycerol free)
Source: E.coli-derived.
Predicted molecular mass: 36 kDa
Unit Definition: One unit of the PNGase F enzyme catalyzes the release of >95% N-linked glycan from 10 ug of denatured RNase B in 10 ul at pH 7.5 in 1 hour at 37°C. One Syd Labs unit of PNGase F activity is equal to 0.015 IUB milliunit.
Formulation: The PNGase F solution (1,800 unit/ug) contains 50 mM Nacl, 5 mM EDTA, and 20 mM Tris-HCl, pH7.5 at 25°C.
Concentration: 500 unit/ul.
Purity: > 95% by SDS-PAGE and SEC-HPLC.
Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied.
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