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Home > Proteins & Peptides > Enzymes > Lyases
Enzymes (EC 4) cleaving various bonds by means other than hydrolysis and oxidation.
    4 Lyases Products
  • BP4200: Recombinant Dr. Nuclease (Benzonase Nuclease Alternative)

    Source: E. coli-derived.
    Specific activity: ≥1.1 x 10^6 U/mg. One unit of Dr. Nuclease is defined as the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
    Purity: ≥95% by SDS-PAGE and ≥99% by SEC-HPLC. It does not contain any antimicrobial preservatives or protein stabilizers except glycerol (of synthetic origin).

     

    BP4200-GMP: Recombinant Dr. Nuclease (Benzonase Nuclease Alternative), GMP Grade

    Source: E. coli-derived.
    Predicted molecular mass: ~28 kDa.
    Specific activity: ≥1.1 x 10^6 U/mg. One unit of Dr. Nuclease is defined as the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
    Purity: ≥95% by SDS-PAGE and ≥99% by SEC-HPLC. It does not contain any antimicrobial preservatives or protein stabilizers except glycerol (of synthetic origin).
    Formulation: 0.2 μM filtered solution (250 units/ul) of 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 2 mM NaCl, 50% Glycerol.
    Endotoxin level: ≤0.01 EU/1000 units following 2020 ChP 1143 USP <85>.
    Protease activity: No detectable protease activity using casein as substrate.
    Residual host cell protein: ≤10.0 ppm by ELISA.
    Mycoplasma: negative by qPCR method.
    Bioburden: negative following 2020 ChP 1105 USP <61>.
    Residual heavy metal: ≤10.0 ppm following 2020 ChP 0821 USP <32>.

  • BP000031-GD5: Recombinant Heparinase I from Flavobacterium heparinum

    Source: E. coli-derived.
    Specific activity >100 IU/mg. One international unit (IU) of recombinant Heparinase I is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0.
    Purity >95% by SDS-PAGE.

  • BP000032-GD6: Recombinant Heparinase II from Flavobacterium heparinum

    Source: E. coli-derived.
    Specific activity >6 IU/mg. One international unit (IU) of recombinant Heparinase II is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 30°C and pH 7.3.
    Purity >95% by SDS-PAGE.

  • BP000033-GD7: Recombinant Heparinase III from Flavobacterium heparinum

    Source: E. coli-derived.
    Specific activity >50 IU/mg. One international unit (IU) of recombinant Heparinase III is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparan sulfate per minute at 30°C and pH 7.3.
    Purity >95% by SDS-PAGE.

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