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Recombinant mouse anti-mouse PD 1 / CD279 monoclonal antibodies, whose variable region sequences are murined from the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14), are produced from mammalian cells. The recombinant rat and chimeric mouse versions of the RMP1-14 antibody are also available.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The rat anti-mouse PD-1 monoclonal antibody (clone No. RMP1-14, rat IgG2a kappa) reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a critical immune checkpoint receptor of the CD28 family. PD-1 interactions with its ligands, PD-L1 and PD-L2, negatively regulate T-cell activation. While traditional anti-mouse PD-1 therapeutic antibodies transiently arrest tumor growth in murine melanoma models, repeated administration of foreign rat-derived proteins often triggers significant anti-drug antibody (ADA) responses in mice, neutralizing biological efficacy during prolonged checkpoint blockade studies.
To overcome this limitation, our recombinant murinized anti-mouse PD-1 antibody (clone RMP1-14.1) represents the ultimate engineering format. It features not only the constant regions of the mouse IgG antibodies but also meticulously murinized variable regions derived from the original rat hybridoma (clone: RMP1-14). Expressed in high-fidelity mammalian cells, this in vivo grade recombinant murinized anti-mouse PD-1 antibody (Cat# PA007162.mm2cLA) combines the specificity of clone RMP1-14.1 with the Fc-silent mouse IgG2c LALAPG (L234A/L235A/P329G) platform. With a certified purity >95%, an ultra-low endotoxin level <1 EU/mg, and a completely azide-free 1x PBS formulation, this fully murinized variant delivers the lowest possible immunogenicity and zero Fc-mediated cytotoxicity, making it the premier choice for long-term chronic in vivo functional assays, Flow Cytometry (FC), and immunohistochemistry (IHC) in syngeneic mouse models.
The in vivo grade recombinant murinized rat anti-mouse PD-1 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells.
Immunogen: The original rat hybridoma (clone name: RMP1-14) was generated by immunizing Sprague Dawley rats with mouse PD-1-transfected BHK cells and using a P3U1 myeloma as the fusion partner.
Clone: RMP1-14.1, the murinized variable region sequences of the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14).
Isotype: mouse IgG2c, kappa.
Applications: immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant rat anti-mouse PD-1 monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
(by AI) Standard chimeric antibodies combine foreign rat variable regions with mouse constant regions, which can still trigger an immune response in recipient animals. (by Syd Labs) Our recombinant murinized anti-mouse PD-1 antibody (clone RMP1-14.1) represents a higher tier of genetic engineering. It features not only the mouse constant regions but also carefully murinized variable regions. By modifying the framework of the variable domain to mimic native mouse sequences while maintaining absolute antigen-binding specificity to murine PD-1 (CD279), this fully murinized format achieves the lowest possible immunogenicity for flawless translation in mouse models.
During chronic or longitudinal animal studies lasting several weeks, repeated administration of traditional rat-derived or partially chimeric antibodies frequently triggers a robust anti-drug antibody (ADA) response in mice. These neutralizing host antibodies accelerate clearance and block the therapeutic interaction with the PD-1 protein, leading to premature experimental failure. Utilizing our recombinant murinized RMP1-14.1 antibody effectively avoids neutralizing ADA development, ensuring sustained systemic drug exposure and maximizing the physiological validity of long-term in vivo functional assays.
Because our PA007162.mm2cLA contains murinized variable regions, it escapes rapid immune-mediated clearance commonly seen with rat IgG2a clones. In standard immunocompetent mice, the typical in vivo half-life of this fully murinized IgG2c platform spans approximately 7 to 10 days, which is significantly longer than raw hybridoma-derived versions. For standard syngeneic tumor models, we recommend a dosing schedule of 100 μg to 200 μg per mouse, administered intraperitoneally (i.p.) twice a week (every 3–4 days), starting when tumors reach an average volume of 50–100 mm³.
To obtain reliable, publication-ready data from immune checkpoint blockades, your negative control must exhibit the exact same effector silencing mutations. For PA007162.mm2cLA (which features the mouse IgG2c LALAPG backbone), you must use a matching recombinant mouse IgG2c LALAPG kappa isotype control (such as Syd Labs Cat# PA007141 variants). Utilizing a non-mutated wild-type mouse IgG2a or IgG2c control will introduce experimental artifacts due to discrepancies in background Fc-receptor binding, invalidating your flow cytometry or tumor growth analysis.
PA007162.mm2cLA is engineered with the mouse IgG2c LALAPG (L234A/L235A/P329G) kappa backbone, making it a perfect genetic match for C57BL/6, C57BL/10, and NOD mice backgrounds. The LALAPG mutation ensures the antibody is entirely silent / Fc-silenced, preventing unwanted ADCC or complement activation. Every lot features a purity >95% by SDS-PAGE and ultra-low endotoxin levels <1 EU/mg in a preservative-free 1x PBS matrix. For short-term usage, store at 2°C to 8°C for up to 1 month. For long-term stability up to 12 months, store in aliquots at -20°C to -70°C in a manual defrost freezer, strictly avoiding repeated freeze-thaw cycles to prevent protein aggregation.
Other Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14.1 / 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / 29F.1A12.1)
Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14.1) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12.1) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2.1) for flow cytometry
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein
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