Anti-Mouse PD-1 Monoclonal Antibodies

Background of Anti-Mouse PD-1 Monoclonal Antibody (29F.1A12)

The rat anti-mouse PD-1 monoclonal antibody 29F.1A12  (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.

Our recombinant 29F.1A12 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 monoclonal antibody (hybridoma clone name or number: 29F.1A12).

PA007163.m2cLA: In Vivo Grade Recombinant Anti-mouse PD-1 Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa Monoclonal Antibody (Clone 29F.1A12.1)

The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells.
Immunogen: The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the mouse PD-1 cDNA followed by the mouse PD-1-Ig fusion protein.
Clone: 29F.1A12.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Isotype: mouse IgG2c, kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 29F.1A12.1) specifically binds to mouse PD-1.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007163.r2a: In Vivo Grade Recombinant Anti-mouse PD-1 Rat IgG2a Kappa Monoclonal Antibody (Clone 29F.1A12.1)

The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (rat IgG2a kappa) was produced in mammalian cells. Its affinity to the mouse PD1 protein is <20 nM.
Immunogen: The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the PD-1 cDNA followed by the PD-1-Ig fusion protein.
Clone: 29F.1A12.1, the same variable region and constant region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Isotype: rat IgG2a, kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 29F.1A12.1) specifically binds to mouse PD-1.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
RRID: AB_3717298

Shipping: The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
3 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

References of Anti-mouse PD-1 Monoclonal Antibody:

STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment.
Koyama, S., et al. Cancer Res. 2016 Mar 1;76(5):999-1008. doi: 10.1158/0008-5472.CAN-15-1439. PMID: 26833127
PD-L1 expression on tumor cells is a biomarker associated with a response to PD-1 blockade treatment. ...Supporting the notion that PD-L1 expression in tumor cells is critical in the response to PD-1 blockade, treatment of the Kras/Lkb1 mouse model with a PD-1 blocking antibody did not show a significant treatment response. ...The therapeutic anti-IL6 antibody significantly inhibited tumor progression as compared with anti PD-1 antibody. ...However, treatment of Kras/Lkb1 tumors with other checkpoint-blocking antibodies against CTLA-4 or a combination of PD-1 and TIM-3 also did not demonstrate any efficacy (data not shown). ...Although therapeutic IL6 blockade improved T-cell function, concurrent therapy combining anti-IL6 and PD-1 treatment did not demonstrate additional benefit as compared with IL6 blockade alone in terms of survival (Supplementary Fig. S6C).
Tags: anti-mouse PD-1 29F.1A12 antibody; anti-mouse PD-1 29F.1A12 mAb

Response to BRAF inhibition in melanoma is enhanced when combined with immune checkpoint blockade.
Cooper, Z. A., et al. Cancer Immunol Res. 2014 Jul;2(7):643-54. doi: 10.1158/2326-6066.CIR-13-0215. PMID: 24903021
One hundred micrograms of anti-PD1 (29F.1A12), 200 ?g anti-PDL1 (10F.9G2), or isotype antibody was administered intraperitoneally at days 1, 3, and 5. ...For PD1 or PDL1 blockade experiments, 100 ?g of rat anti-mouse PD1 (29F.1A12), 200 ?g of anti-PDL1 (10F.9G2; ref. 25), or isotype antibody (LTF-2) were administered intraperitoneally on days 1, 3, and 5 following BRAFi or control chow initiation. ...The anti-PD1 (29F.1A12) blocking antibody blocks the binding of PD1 to its two ligands, PDL1 and PDL2. The anti-PDL1 (10F.9G2) blocking antibody blocks interaction of PDL1 with both of its binding partners, PD1 and CD80 (B7-1; ref. 27). ...FFPE tumor specimens were deparaffinized in xylene and hydrated in a series of ethanol dilutions. ...A tumor-infiltrating lymphocyte (TIL)?enrichment protocol was used for both human and mouse tumors.
Tags: anti-mouse PD-1 29F.1A12 antibody in vivo; anti-mouse PD-1 29F.1A12 in animal model

Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors.
Duraiswamy, J., et al. Cancer Res. 2013 Jun 15;73(12):3591-603. doi: 10.1158/0008-5472.CAN-12-4100. PMID: 23633484
It is well established that tumors use programmed death-1 (PD-1) and CTL antigen-4 (CTLA-4) inhibitory pathways to silence the immune system. ...PD-1 binds 2 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). ...Upregulation of PD-L1 occurs on a wide variety of human tumors suggesting that cancer cells coopt the PD-1/PD-L1 inhibitory pathway to evade the host immune response. ...Immunoregulatory pathways involving PD-1, CTLA-4, and their ligands are highly complex. ...However, the intricacies of each pathway and cross-talk between them make it unlikely that blockade of PD-1 or CTLA-4 would have identical effects.
Tags: anti-mouse PD-1 29F.1A12 antibody in animal model; anti-mouse PD-1 29F.1A12 mAb in animal model

Role of the immune modulator programmed cell death-1 during development and apoptosis of mouse retinal ganglion cells.
Chen, L., et al. Invest Ophthalmol Vis Sci. 2009 Oct;50(10):4941-8. doi: 10.1167/iovs.09-3602. PMID: 19420345
PD-1 (CD279) is a key immunoregulatory receptor, inducibly expressed on T cells, B cells, NK T cells, activated monocytes, and dendritic cells. ...PD-1 transduces an inhibitory signal when engaged in combination with the T cell receptor (TCR). ...During the establishment of central tolerance, PD-1 is expressed on developing thymocytes as they progress through several maturational stages in which PD-1 signaling modifies signaling thresholds in thymocytes during positive and negative selection stages of maturation. ...Embryonic and adult CD1 mice were used for PD-1 blocking experiments. ...For the PD-1 blocking study, each of three experimental repeats examined four retinal explants; for each explant, five sections were costained with caspase-3, Brn3a, and DAPI.
Tags: anti-mouse PD-1 29F.1A12 in cancer research; anti-mouse PD-1 29F.1A12 in mouse tumor model

CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation.
Good-Jacobson, K. L., et al. J Immunol. 2012 May 1;188(9):4217-25. doi: 10.4049/jimmunol.1102885. PMID: 22450810
Germinal center (GC) B cells and T follicular helper (TFH) cells interact in the production of high-affinity long-lived plasma cells (PCs) and memory B cells, although the mechanisms regulating the formation of these long-lived populations remain unclear. ...Because CD80 is one of the few markers shared by human and murine memory B cells, we investigated its role in the development of GCs, memory cells, and PCs. ...In CD80-deficient mice, fewer long-lived PCs were generated upon immunization compared with that in B6 controls. ...In concert, the absence of CD80 resulted in an increase in apoptotic GC B cells during the contraction phase of the GC. ...Mixed bone marrow chimeras demonstrated a B cell-intrinsic requirement for CD80 expression for normal TFH cell and PC development.
Tags: anti-mouse PD-1 29F.1A12 antibody in mouse tumor model; anti-mouse PD-1 29F.1A12 mAb in mouse tumor model

Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses.
Liang, S. C., et al. Eur J Immunol. 2003 Oct;33(10):2706-16. doi: 10.1002/eji.200324228. PMID: 14515254
Here, we report the expression of PD-1, PD-L1, and PD-L2 at the protein level in vivo in both hematopoietic and non-hematopoietic tissues of naive mice and in two models of autoimmunity. ...The differential expression of PD-L1 and PD-L2 suggests distinct functions for these PD-1 ligands. ...To examine the protein expression of PD-1 and its ligands, we generated antibodies to murine PD-1 (clone 29F.1A12) and PD-L1 (clone 10F.5C5). ...We also analyzed the ability of these new mAb to block PD-1 interactions with PD-L1 or PD-L2 in an in vitro blocking assay. ...PD-L1-transfected CHO cells were incubated with the 10F.5C5 mAb, followed by addition of the biotinylated PD-1 Ig fusion protein.
Tags: bioactivity of anti-mouse PD-1 29F.1A12; anti-mouse PD-1 29F.1A12 mAb of low endotoxin

For more references about Anti-mouse PD-1 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14.1 / 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / 29F.1A12.1)

Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14.1) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12.1) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2.1) for flow cytometry

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein

Questions and Answers about anti-mouse PD-1 antibody (Clone 29F.1A12):

Question: Which anti-mouse PD1 antibody works the best?
Answer: (by AI) There are several anti-mouse PD1 antibodies available in the market. Bio X Cell offers three different anti-mouse PD-1 monoclonal antibodies: Clones RMP1-14, 29F.1A12, and J43. All three of these antibodies are commonly used to block PD-1 signaling in vivo in murine tumor models and other mouse models. These three clones all have extensive multi-year publication records supporting them. Leinco Technologies offers four different anti-PD-1 monoclonal antibodies: 29F.1A12, RMP1-14, J43, and 5C4-B83. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells). (by Syd Labs) Syd Labs offers anti-mouse PD-1 / CD279 monoclonal antibodies based on the sequences of clones RMP1-14 and 29F.1A12. Syd Labs provides in vivo grade recombinant antibodies including engineered antibodies for the clones RMP1-14 and 29F.1A12.

Question: Do you produce Fc-silenced 29F.1A12 antibody?
Answer: We provide recombinant 29F.1A12 antibodies including the Fc-silenced or Fc silent versions of recombinant 29F.1A12 antibodies. It is very easy for us to produce the Fc-silenced or Fc silent versions of recombinant 29F.1A12 antibodies no matter which isotypes they are based, for example, mIgG2c LALAPG.

Question: What are the published applications of the 29F.1A12 antibody?
Answer: It was reported that the blockade of the PD-1 protein expressed on TME-infiltrating macrophages and natural killer (NK) cells by 29F.1A12 results in tumor growth suppression and prolonged survival. It is also reported that the 29F.1A12 antibody recognizes the PD-1 surface protein on B16-F10 and other murine tumor lines, suppresses cancer cell-intrinsic PD-1 receptor signaling, and inhibits resultant tumor growth in three-dimensional (3D) culture and tumor-bearing mice. In the paper "Distinct antibody clones detect PD-1 checkpoint expression and block PD-L1 interactions on live murine melanoma cells", it was shown that the anti-PD-1 29F.1A12 monoclonal antibody recognizes the PD-1 surface protein on live B16-F10 melanoma cells, live YUMM melanoma variant lines, and overlapping WT murine B16-F10 and YUMM melanoma and T-cell subsets by FACS. In contrast to the RMP1-30 antibody, The 29F.1A12 PD-1 blocking antibody inhibits PD-1 interactions with PD-L1 on live B16-F10 melanoma cells. The RMP1-14 clone, but not 29F.1A12 or RMP1-30, is useful for pulling down (IP) and sequencing PD-1 from B16-F10 and T-cell cultures, consistent with the previous observations that individual antibodies differ in their suitability for specific assay types. 29F.1A12 binding to B16-F10 cells was increased in 3D versus 2D settings.

Alternative Names of anti-mouse PD 1 antibody:

CD279 antibody?anti-mouse CD279 antibody?anti-mouse PD 1 antibody?anti-mouse PD-1 antibody?anti-mouse PD1 antibody?Programmed cell death 1 antibody?Programmed cell death 1 protein antibody?Programmed cell death protein 1 antibody?Protein PD 1 antibody?Protein PD-1 antibody?cluster of differentiation 279