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Recombinant anti-mouse PD1 monoclonal antibodies, which share the same variable region sequences with the rat anti-mouse PD-1 / CD279 monoclonal antibody (clone number: 29F.1A12), are produced from mammalian cells and good for in vitro and in vivo studies.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The rat anti-mouse PD-1 monoclonal antibody (clone No. 29F.1A12.1, rat IgG2a kappa) reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse Pdcd1 gene, a prominent immune checkpoint receptor belonging to the CD28 family of the Ig superfamily. PD-1 functions through downstream signaling pathways via its two B7-family ligands, PD-L1 and PD-L2. Extensive preclinical research demonstrates that in syngeneic mouse models of melanoma and other malignancies, T-cell mediated cytotoxicity can be reactivated and tumor growth can be transiently arrested via targeted anti-mouse PD-1 antibody treatment. The 29F.1A12.1 blocking antibody effectively inhibits the interaction between the PD-L1/PD-L2 proteins and their receptor PD-1 protein to restore anti-tumor immunity.
Our recombinant 29F.1A12.1 antibody is meticulously engineered with 100% precise amino acid sequences of the variable regions derived from the original rat hybridoma (clone: 29F.1A12), which was uniquely generated by immunizing rats with mouse PD-1 cDNA followed by the mouse PD-1-Ig fusion protein. Expressed in high-fidelity mammalian cells, this in vivo grade recombinant anti-mouse PD-1 antibody eliminates hybridoma gene drift, providing a high binding affinity <20 nM and purity >95%. Available in versatile recombinant formats including the classic rat IgG2a and the Fc-silent mouse IgG2c LALAPG (L234A/L235A/P329G) platforms, this ultra-pure, low endotoxin (<1 EU/mg), and azide-free formulation is fully cross-validated for Western Blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and rigorous long-term in vivo functional assays.
The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells.
Immunogen: The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the mouse PD-1 cDNA followed by the mouse PD-1-Ig fusion protein.
Clone: 29F.1A12.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Isotype: mouse IgG2c, kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 29F.1A12.1) specifically binds to mouse PD-1.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibody (rat IgG2a kappa) was produced in mammalian cells. Its affinity to the mouse PD1 protein is <20 nM.
Immunogen: The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the PD-1 cDNA followed by the PD-1-Ig fusion protein.
Clone: 29F.1A12.1, the same variable region and constant region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: 29F.1A12).
Isotype: rat IgG2a, kappa.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 29F.1A12.1) specifically binds to mouse PD-1.
Applications: western blot (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
RRID: AB_3717298
Shipping: The in vivo grade recombinant anti-mouse PD-1 / CD279 monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
3 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
(by AI) While multiple clones can block murine PD-1 pathways in vivo, clone 29F.1A12.1 stands out for its extensive multi-year publication record and its unique dual-compatibility in both functional assays and western blotting. (by Syd Labs) Our recombinant anti-mouse PD-1 antibody (clone 29F.1A12.1) is generated via genetic engineering from the original hybridoma (produced via non-traditional cDNA and protein immunization). This ensures unparalleled structural stability. Unlike other clones that are primarily restricted to in vivo usage, our recombinant 29F.1A12.1 is fully cross-validated for high-sensitivity Western Blot (WB), Flow Cytometry (FC), and immunohistochemistry (IHC), providing researchers with a seamless transition from protein expression verification to animal immunotherapy profiling.
Syd Labs offers the comprehensive matrix of recombinant 29F.1A12.1 formats to match specific host-engineered controls and eliminate non-specific Fc receptor activation:
1. Rat IgG2a (PA007163.r2a): The recombinant counterpart of the native hybridoma, offering a binding affinity <20 nM, ideal for standard checkpoint inhibition.
2. Mouse IgG2c / IgG2a LALAPG (PA007163.m2cLA / PA007163.m2aLA): Engineered with an Fc silent / Fc-silenced platform (L234A/L235A/P329G mutations). Select IgG2c LALAPG if you are using C57BL/6 or NOD mice, and IgG2a LALAPG for BALB/c strains to prevent ADCC-mediated target cell depletion and minimize anti-drug antibody (ADA) interferences.
3. Mouse IgG1-D265A (PA007163.m1DA): An alternative ultra-pure alternative for specialized effector-function silencing.
4. Rabbit IgG (PA007163.rt): A highly rare recombinant rabbit chimeric format, specifically built for advanced multiplexing panels or secondary staining controls where cross-reactivity with rat/mouse hosts must be strictly avoided.
In standard syngeneic mouse models, the in vivo half-life of our recombinant Rat IgG2a (PA007163.r2a) and chimeric mIgG2c LALAPG (PA007163.m2cLA) formats is approximately 6 to 9 days. Because clone 29F.1A12.1 possesses an engineered binding affinity <20 nM, it delivers robust and predictable target saturation. For standard tumor models (such as B16-F10 melanoma or Lewis lung carcinoma), the established and published dosing regimen is 200 μg per injection diluted in PBS (2 mg/mL), administered via intraperitoneal (i.p.) injection every 3 to 4 days (twice a week) for a duration of 2 to 3 weeks, beginning around day 8–9 post-tumor inoculation.
Due to the extensive range of host frameworks available for our 29F.1A12.1 clone, matching the precise engineered negative control is a strict requirement to eliminate background artifacts:
• For the native Rat IgG2a variant (PA007163.r2a), pair with a standard recombinant Rat IgG2a isotype control.
• For the Fc-silenced Mouse IgG2c LALAPG format (PA007163.m2cLA) or Mouse IgG2a LALAPG format (PA007163.m2aLA), you must avoid wild-type controls; instead, explicitly pair them with an Fc-silent recombinant Mouse IgG2c/IgG2a LALAPG kappa isotype control (such as Syd Labs Cat# PA007141 series).
• For the unique Rabbit IgG format (PA007163.rt), ensure your control group receives a matching recombinant Rabbit IgG constant region control to properly baseline your multiplexed staining or in vitro assays.
Every batch of our in vivo grade recombinant anti-mouse PD-1 antibody (clone 29F.1A12.1) undergoes stringent quality control. It is supplied as a 0.2 μM filtered solution in 1x PBS, completely azide-free and preservative-free to guarantee no cellular toxicity. The endotoxin level is certified to be less than 1 EU/mg (frequently <0.05 EU/mg for large-scale batches) via LAL assay, with a verified protein purity >95% via SDS-PAGE. To maintain long-term stability and prevent protein aggregation, store short-term at 2°C to 8°C (up to 1 month). For long-term storage up to 12 months, preserve in working aliquots at -20°C to -70°C in a manual defrost freezer. Strictly avoid repeated freeze-thaw cycles, which can induce physical fragmentation or precipitation.
Related Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14.1 / 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / 29F.1A12.1)
Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14.1) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12.1) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2.1) for flow cytometry
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein
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