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Home > Antibodies > Anti-Mouse Antibodies for Flow Cytometry > Anti-Mouse PD-L1 Monoclonal Antibodies for Flow Cytometry

Anti-Mouse PD-L1 Monoclonal Antibodies for Flow Cytometry

Programmed Death Ligand 1, B7-H1, CD274, 10F.9G2

Catalog No. Product Name Size List Price (US$) Quantity
PA007506.r2b Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Rat IgG2b Kappa 100 ug 55.00
PA007506.r2b Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Rat IgG2b Kappa 500 ug 100.00
PA007506.h1Fs Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa 100 ug 110.00
PA007506.h1Fs Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa 500 ug 200.00
PA007735.h1Fs-b Biotinylated Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa 100 ug 110.00
PA007735.h1Fs-b Biotinylated Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa 500 ug 220.00
Description

PA007506.r2b: Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody. 
Clone: 10F.9G2.1.
Isotype:  Rat IgG2b Kappa.
Source: The anti-mouse PD-L1 monoclonal antibody (clone: 10F.9G2.1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10F.9G2.1 antibody specifically binds to mouse PD-L1 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007506.h1Fs: Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: 10F.9G2.1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse PD-L1 monoclonal antibody (clone: 10F.9G2.1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 10F.9G2.1 antibody specifically binds to mouse PD-L1 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007735.h1Fs-b: Biotinylated Recombinant Anti-mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2.1), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: 10F.9G2.1.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-mouse PD-L1 monoclonal antibody (clone: 10F.9G2.1) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant 10F.9G2.1 antibody specifically binds to mouse PD-L1 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The 10F.9G2.1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

Background

The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein.
Our recombinant 10F.9G2 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD L1 monoclonal antibody (hybridoma clone name or number: 10F.9G2).

References of Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2)

Zhang J, et al. J Immunother Cancer. 2019 Aug 23;7(1):223. doi: 10.1186/s40425-019-0693-y. PMID: 31446896
TRAMP/MICB male mice of 26 to 28 week-old were assigned into four cohorts with similar distribution of serum sMIC (Additional file 1: Figure S1) receiving therapy with intraperitoneal (i.p.) injection of sMIC-targeting nonblocking monoclonal antibody B10G5 [28] or anti-PD-L1 (clone 10F.9G2, BioXCell) antibody or isotype control IgG (cIgG) respectively at a respective dose of 4.0 mg/kg body weight twice weekly. Combination of the following antibody was used for cell surface or intracellular staining to define populations of NK, CD8, and subsets of CD4 T cells: CD3e (clone 145-2c11), CD8a (clone 53-6.7), CD4 (clone GK1.5), NK1.1 (clone PK136), NKG2D (clone CX5), CD44 (clone eBio4B10), CD11c (clone N418), MHCII (clone M5/114.15.2), CD80 (clone 16-10A1), CD86 (clone PO3) and CD40 (clone 1C10). Multicolored Flow cytometry analyses were performed on an LSR II (BD). Representative histograms from flow cytometry analyses demonstrating the percentage of PD-L1+ tumor cells from TRAMP/MICB mice. Representative histograms from flow cytometry analyses of CD40, CD80 and CD86 expression on DCs from tumor-draining lymph nodes and tumor beds.
(Tags: anti-mouse CD274 (PD-L1) [Clone 10F.9G2] - purified; 10F.9G2 anti-mouse PD-L1 antibody)
For more technical references or data sheets regarding the Recombinant Anti-Mouse PD-L1 Monoclonal Antibody (Clone: 10F.9G2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14.1 / 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / 29F.1A12.1)

Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14.1) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12.1) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2.1) for flow cytometry

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein

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