Anti-Mouse PD1 Monoclonal Antibodies for Flow Cytometry

PA007504.r2a: Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: RMP1-14.1), Rat IgG2a Kappa

Recombinant Rat IgG2a Monoclonal Antibody. 
Clone: RMP1-14.1.
Isotype:  Rat IgG2a Kappa.
Source: The anti-mouse PD1 monoclonal antibody (clone: RMP1-14.1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant RMP1-14.1 antibody specifically binds to mouse PD1 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007504.h1Fs: Recombinant Anti-mouse PD1 Monoclonal Antibody (Clone: RMP1-14.1), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: RMP1-14.1.
Isotype: Human IgG1 kappa.
Source: The anti-mouse PD1 monoclonal antibody (clone: RMP1-14.1) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant RMP1-14.1 antibody specifically binds to mouse PD1 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The RMP1-14.1 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Background

The rat anti-mouse PD-1 monoclonal antibody (clone No. RMP1-14, rat IgG2a kappa) reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD 1 antibody and the anti-mouse PD L1 antibody which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14 antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein.
Our recombinant RMP1-14 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody (hybridoma clone name or number: RMP1-14). The murinized anti-mouse PD-1 recombinant antibody has not only the constant regions of the mouse IgG antibodies but also the murinized variable regions.

References of anti-mouse PD-1 antibody (RMP1-14) for flow cytometry

A Comparison of Murine PD-1 and PD-L1 Monoclonal Antibodies.
Bu, M. T., et al. (2022). Monoclon Antib Immunodiagn Immunother. 2022 Aug;41(4):202-209. doi: 10.1089/mab.2021.0068. PMID: 35925787
Unconjugated and fluorophore-conjugated PD-1 mAbs (1A12, rat IgG2a; RMP1-14, rat IgG2a; and RMP1-30, rat IgG2b) and PD-L1 mAbs (10F.9G2, rat IgG2b; MIH6, rat IgG2a) were purchased from...Owing to the widespread use of both PD-1 mAbs 1A12 and RMP1-14 in experimental mouse models, we compared the avidities of these two mAbs. ...The 1A12 antimouse PD-1 mAb bound to mPD-1–transfected Jurkat cells in a dose-dependent manner with an apparent affinity of 0.42 nM, while RMP1-14 antibody bound with a much weaker apparent affinity of 28.8 nM. ...This result was also seen with 1A12 and RMP1-14 mAbs from a different vendor (Fig. S1). ...In case the lower avidity of RMP-1-14 (anti-mouse CD279 antibody) was a consequence of transfection, we assayed the antibody binding to exhausted murine CD8 T cells naturally expressing PD-1...
Tags: purified anti-mouse CD279 (PD-1) (RMP1-14); anti-mouse CD279 mAb for flow cytometry

Dying cells expose a nuclear antigen cross-reacting with anti-PD-1 monoclonal antibodies.
Metzger, P., et al. (2018). Sci Rep. 2018 Jun 11;8(1):8810. doi: 10.1038/s41598-018-27125-6. PMID: 29892077
We assessed PD-1 expression by flow cytometry and compared the two mainly used antibody clones (29 F.1A12 and RMP1-14 antibodies), which were also included in the above-mentioned study. ...We observed PD-1 staining exclusively in the dead cell fraction for both clones 29 F.1A12 and RMP1-14 mAb, respectively...
Tags: activity of anti-mouse CD279; binding to mouse CD279 (mCD279)

The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent.
Grasselly, C., et al. (2018). Front Immunol. 2018 Oct 9;9:2100. doi: 10.3389/fimmu.2018.02100. PMID: 30356816
Anti-PD1 antibody(clone RMP1-14) or anti-PDL1 (clone 10F.9G2) mAb (12,5 mg/kg, ip, q1wk) were administered in combination with (A,C) methotrexate (1 mg/kg, ip, q1wk), vinblastine (0.1 mg/kg, ip, q1wk), doxorubicin(1 mg/kg, ip, q1wk) and cisplatin(1 mg/kg,ip, q1wk, MVAC) in SC bladder cancer MB49 and MBT-2, and with (B) cyclophosphamide (CTX, 100 mg/kg, ip, q1wk) and doxorubicin (DOX, 2 mg/kg, ip, q1wk) in SC metastatic breast cancer 4T1, and with (D) capecitabine (CAPE, 250 mg/kg, po 5 days a week) and oxaliplatin (OXA, 5 mg/kg, ip, q1wk) in SC colorectal cancer MC38. ...Effect of anti-PD1 antibody (clone RMP1-14) or anti-PDL1 (clone 10F.9G2) antibody, 12,5 mg/kg, i.p., q1wk, in combination with cyclophosphamide (CTX) 100 mg/kg, i.p, q1wk and doxorubicin (DOX) 2 mg/kg, i.p, q1wk in SC metastatic breast cancer 4T1 (A), methotrexate 1mg/kg, i.p, q1wk and vinblastine 0,1 mg/kg, i.p, q1wk and doxorubicin 1 mg/kg, i.p, q1wk and cisplatin 1 mg/kg i.p, q1wk (MVAC) in SC bladder cancer MB49 (B), Data are shown as Mean + SEM, n = 5 to 6 (A), n = 6 (B).
Tags: function of anti-mouse CD279 antibody; rat anti-mouse CD279 (PD-1)

Type I MET inhibitors cooperate with PD-1 blockade to promote rejection of hepatocellular carcinoma.
DeAzevedo, R., et al. (2024). J Immunother Cancer. 2024 Oct 30;12(10):e009690. doi: 10.1136/jitc-2024-009690. PMID: 39477243
On days 7, 11, 15, and 19, mice received 250 ug anti-PD-1 antibody RMP1-14 i.p. or isotype control antibody. ...On days 7, 11, 15, and 19, mice received 250 ug anti-PD-1 mAb RMP1-14 i.p. or isotype control antibody. ...In this system, mice were treated when measurable tumors emerged with 10 doses of c-Met inhibitor and 5 doses of the PD-1 antibody RMP1-14. ...On days 2, 4, 6, 8, and 10 post-palpation, mice received 250 ug anti-PD-1 RMP1-14 antibody i.p. or isotype control antibody. ...On days 2, 4, 6, 8, and 10 post-palpation, mice received 250 ug anti-PD-1 RMP1-14 mAb i.p. or isotype control antibody.
Tags: anti-mouse CD279 antibody for flow cytometry; anti-mouse CD279 mAb

Impact of mouse model tumor implantation site on acquired resistance to anti-PD-1 immune checkpoint therapy.
Denis, M., et al. (2023). Front Immunol. 2023 Jan 10;13:1011943. doi: 10.3389/fimmu.2022.1011943. PMID: 36703964
When the tumor volume reached 150 mm3, mice were randomized and received first treatment of anti-PD-1 antibody (RMP1-14, 12.5 mg/kg per week, intraperitoneal (IP)). ...The mice are randomized based on their weight and receive 48 hours after the operation their first anti-PD-1 treatments (RMP1-14, 12.5 mg/kg per week, IP)...
Tags: mouse CD279-specific antibody; anti-mouse CD279 RMP1-14

For more references about anti-mouse PD-1 antibody (clone RMP1-14) for flow cytometry, please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)

Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein