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Recombinant anti-mouse PD 1 / CD279 monoclonal antibodies, which share the same variable region sequences with the rat anti-mouse PD-1 antibody (clone number: RMP1-14), are produced from mammalian cells. The recombinant rat IgG2a isotype control available.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The rat anti-mouse PD-1 monoclonal antibody (clone No. RMP1-14.1, rat IgG2a kappa) reacts with the mouse PD-1 protein (CD279 or programmed death-1) encoded by the mouse pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma and colon cancer, tumor growth can be transiently arrested via anti-mouse PD-1 antibody treatment and anti-mouse PD-L1 antibody interventions which block the therapeutic interaction between the PD-L1 protein and its receptor PD-1 protein. The RMP1-14.1 blocking antibody effectively blocks the reciprocal binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein during in vivo checkpoints blocking.
Our recombinant RMP1-14.1 antibody is engineered with 100% precise amino acid sequences of the variable regions derived from the original rat hybridoma (clone: RMP1-14). Expressed in advanced mammalian cells, this in vivo grade recombinant anti-mouse PD-1 antibody delivers superior batch-to-batch consistency with a high binding affinity <2 nM and purity >95%. Available in versatile formats including the classic rat IgG2a and the Fc-silent mouse IgG2c LALAPG (L234A/L235A/P329G) platforms, this low endotoxin (<1 EU/mg), azide-free formulation eliminates non-specific cell activation, making it ideal for immunohistochemistry (IHC), Flow Cytometry (FC), in vitro neutralization, and long-term in vivo functional assays.
The in vivo grade recombinant rat anti-mouse PD-1 monoclonal antibody (rat IgG2a kappa) was produced in mammalian cells. Its affinity to the mouse PD-1 protein is <2 nM.
Immunogen: The original rat hybridoma (clone name: RMP1-14) was generated by immunizing Sprague Dawley rats with mouse PD-1-transfected BHK cells and using a P3U1 myeloma as the fusion partner.
Clone: RMP1-14.1, the same variable region and constant region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14).
Isotype: rat IgG2a, kappa.
Source: The anti-mouse PD-1 monoclonal antibody (clone: RMP1-14.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: RMP1-14) specifically binds to mouse PD-1.
Applications: immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
RRID: AB_3697971
The in vivo grade recombinant rat anti-mouse PD-1 monoclonal antibody (mouse IgG2c-LALAPG kappa) was produced in mammalian cells. Its affinity to the mouse PD-1 protein is <2 nM.
Immunogen: The original rat hybridoma (clone name: RMP1-14) was generated by immunizing rats with mouse PD-1-transfected BHK cells.
Clone: RMP1-14.1, the same variable region sequences as the rat anti-mouse PD-1 monoclonal antibody (clone number: RMP1-14).
Isotype: mouse IgG2c, kappa.
Source: The anti-mouse PD-1 monoclonal antibody (clone: RMP1-14.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: RMP1-14) specifically binds to mouse PD-1.
Applications: immunohistochemistry (IHC), Flow Cytometry (FC), and various in vitro and in vivo functional assays.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant rat anti-mouse PD-1 monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.
(by AI) Traditional hybridoma-derived antibodies often suffer from hybridoma gene drift and batch-to-batch variability, which can compromise the reproducibility of long-term preclinical studies. (by Syd Labs) Our recombinant anti-mouse PD-1 antibody (clone RMP1-14.1) is engineered using 100% verified genetic sequences and expressed in advanced mammalian cell culture (CHO or HEK293 cells). This ensures absolute sequence integrity, exceptional batch reproducibility, and scalable production. Purified under rigorous quality controls, our in vivo grade recombinant RMP1-14.1 features high binding affinity <2 nM and a purity >95%, providing clean data without hybridoma-associated cellular contaminants.
Syd Labs provides versatile recombinant engineered formats of clone RMP1-14.1 tailored to specific mouse strains and biological applications to prevent immunogenicity and non-specific Fc interactions:
1. Rat IgG2a (PA007162.r2a): Features the classic variable and constant regions matching the original hybridoma. It is widely cited for standard, short-term in vivo checkpoint blockade.
2. Mouse IgG2c LALAPG (PA007162.m2cLA): This format utilizes a mouse constant region with LALAPG mutation (L234A/L235A/P329G) to create an Fc silent / Fc-silenced variant. Because inbred strains like C57BL/6, C57BL/10, and NOD express the IgG2c isotype rather than IgG2a, using the mIgG2c LALAPG format is highly recommended for these backgrounds during long-term or chronic administration to eliminate antibody-dependent cellular cytotoxicity (ADCC) and minimize anti-drug antibody (ADA) responses.
In standard immunocompetent mice, the in vivo half-life of our recombinant Rat IgG2a (PA007162.r2a) and chimeric mIgG2c LALAPG (PA007162.m2cLA) formats ranges from 5 to 8 days, depending on the metabolic rate and tumor burden of the host. For established syngeneic murine tumor models (such as MC38 or CT26), the standard validated in vivo dosing regimen is 100 μg to 200 μg per mouse, administered via intraperitoneal (i.p.) injection every 3 to 4 days (twice a week). Treatment typically initiates once palpable subcutaneous tumors reach an average volume of 50–100 mm³ and continues for 3–4 weeks or until experimental endpoints.
To secure reliable, publication-ready data from in vivo immune checkpoint blockades, your negative control must match the exact engineered backbone of your targeting antibody. If you are administering the native Rat IgG2a platform (PA007162.r2a), you must pair it with a recombinant Rat IgG2a isotype control. Crucially, if your study utilizes the Fc-silenced Mouse IgG2c LALAPG format (PA007162.m2cLA), using a wild-type control will invalidate your results due to background Fc-receptor activation differences; you must pair it with an Fc-silent recombinant Mouse IgG2c LALAPG kappa isotype control (such as Syd Labs Cat# PA007141 variants).
Our in vivo grade recombinant anti-mouse PD-1 antibody (clone RMP1-14.1) is explicitly formulated for highly sensitive physiological environments. It is supplied as a 0.2 μM filtered solution in 1x PBS, entirely azide-free and preservative-free to ensure zero cytotoxicity. The endotoxin level is strictly maintained at less than 1 EU/mg of protein as determined by the LAL method. While optimized as a potent functional blocking agent in murine syngeneic tumor models, its exceptional specificity makes it fully cross-compatible with Flow Cytometry (FC), immunohistochemistry (IHC), and in vitro T-cell neutralization assays. For optimal stability, store at 2–8°C for short-term use (up to 1 month) or in working aliquots at -20°C to -70°C in a manual defrost freezer for up to 12 months, avoiding repeated freeze-thaw cycles to prevent protein aggregation.
Related Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
Syd Labs provides the following in vivo grade recombinant anti-mouse PD-L1 / PD-1 monoclonal antibodies:
In vivo grade recombinant anti-mouse PD1 monoclonal antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 monoclonal antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 monoclonal antibodies (Clone 10F.9G2.1)
Syd Labs provides the following in vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies:
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone RMP1-14.1 / 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 / PD-1 bispecific antibodies (Clone 29F.1A12.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone RMP1-14.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / RMP1-14.1)
In vivo grade recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (Clone 29F.1A12.1 / 10F.9G2.1)
In vivo grade recombinant anti-mouse PD-L1 / PD-1 bispecific antibodies (Clone 10F.9G2.1 / 29F.1A12.1)
Syd Labs provides the following recombinant anti-mouse PD-1 / PD-L1 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: RMP1-14.1) for flow cytometry
Recombinant Anti-mouse PD-1 monoclonal antibodies (Clone: 29F.1A12.1) for flow cytometry
Recombinant Anti-mouse PD-L1 monoclonal antibodies (Clone: 10F.9G2.1) for flow cytometry
Recombinant PD1 and PDL1 Proteins:
Recombinant Human PD-1/PDCD1 Proteins
Biotinylated Recombinant Human PD-1/PDCD1 Proteins
Recombinant Mouse PD-1/PDCD1 Proteins
Biotinylated Recombinant Mouse PD-1/PDCD1 Proteins
Recombinant Cynomolgus PD-1/PDCD1 Proteins
Recombinant Canine PD-1/PDCD1 Proteins
Biotinylated Cynomolgus/Rhesus macaque PD-L1 Protein
Biotinylated Human PD-L1 Protein
Biotinylated Mouse PD-L1 Protein
Cynomolgus PD-L1 Protein
Cynomolgus/Rhesus macaque PD-L1 Protein
Human PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein
Alternative Names of anti-mouse PD 1 antibody:
CD279 antibody, anti-mouse CD279 antibody, anti-mouse PD 1 antibody, anti-mouse PD-1 antibody, anti-mouse PD1 antibody, Programmed cell death 1 antibody, Programmed cell death 1 protein antibody, Programmed cell death protein 1 antibody, Protein PD 1 antibody, Protein PD-1 antibody, cluster of differentiation 279
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